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Advancements in Regenerative Medicine Research

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 879

Special Issue Editor


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Guest Editor
Precision Medicine Program, College of Graduate Studies, Midwestern University, Downers Grove, IL 60515, USA
Interests: regeneration; cell signaling; developmental biology; mechanotransduction; laser therapy; in vivo model systems

Special Issue Information

Dear Colleagues,

Regenerative medicine is a rapidly evolving and interdisciplinary field dedicated to the repair, replacement, or regeneration of human cells, tissues, and organs, to restore normal function. Grounded in stem cell biology, tissue engineering, and molecular signaling, this field holds transformative potential for the treatment of a wide range of conditions, including neurodegenerative disorders, musculoskeletal injuries, cardiovascular disease, diabetes, and organ failure. Despite growing interest and significant progress, many challenges remain, such as the limited integration of engineered tissues, immune rejection, safety concerns, and gaps in understanding the endogenous mechanisms of tissue repair.

This Special Issue aims to showcase recent breakthroughs and address ongoing challenges in regenerative medicine, with an emphasis on mechanistic discoveries, innovative technologies, and translational research that are advancing the field. By bringing together diverse scientific perspectives, this Special Issue aims to advance our understanding of regenerative processes, identify new therapeutic approaches, and foster the development of personalized, precision-based regenerative treatments.

Dr. Rosa V. Ventrella
Guest Editor

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Keywords

  • regenerative medicine
  • tissue repair
  • wound healing
  • stem cells
  • cell differentiation
  • cell signalling
  • gene regulation
  • mechanotransduction
  • precision medicine

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Published Papers (1 paper)

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Research

35 pages, 4696 KB  
Article
Neuronal Differentiation and Exosome Profiling of Dental Pulp Stem Cells: Unveiling Their Potential for Nerve Repair
by Bruna Lopes, Patrícia Sousa, Alícia de Sousa Moreira, Ana Catarina Sousa, Alexandra Rêma, Luís Atayde, António J. Salgado, Stefano Geuna, Rui Alvites and Ana Colette Maurício
Int. J. Mol. Sci. 2025, 26(19), 9723; https://doi.org/10.3390/ijms26199723 - 6 Oct 2025
Viewed by 636
Abstract
Peripheral nerve injuries remain a major clinical problem, and cell-free therapies using stem cell-derived bioproducts have emerged as promising alternatives. This study evaluated the influence of neurogenic differentiation and passage number on the secretomic and exosomal profile of human dental pulp stem cells [...] Read more.
Peripheral nerve injuries remain a major clinical problem, and cell-free therapies using stem cell-derived bioproducts have emerged as promising alternatives. This study evaluated the influence of neurogenic differentiation and passage number on the secretomic and exosomal profile of human dental pulp stem cells (hDPCSs). Conditioned media from undifferentiated and neurodifferentiated hDPSCs, and exosomes derived from undifferentiated hDPSCs at passages 4 and 7, were analyzed using multiplex immunoassays, RT-PCR, and scanning electron microscopy (SEM). Neurodifferentiated hDPSCs at early passages secreted higher levels of neurotrophic, angiogenic and immunomodulatory factors, including FGF-2, IL-6, IL-8, and PDGF-AA. Exosomes from early-passage undifferentiated cells showed a more abundant and relevant neuroregenerative mRNA cargo in comparison to the later passages. Both cell types and exosomes adhered to the Reaxon® nerve guidance conduit, confirming the permissive nature of the materials regarding cells and cellular products, allowing adhesion and survival. Neurite outgrowth assays performed on neurodifferentiated hDPSCs confirmed functional neural behavior. In later passages, a decline in secretory and exosomal activity was noted. These results highlight the relevance of early-passage hDPSCs as a source of bioactive factors and support their application in cell-free approaches for peripheral nerve regeneration. Full article
(This article belongs to the Special Issue Advancements in Regenerative Medicine Research)
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