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Gene Editing for Cereal Crops

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 1 December 2025 | Viewed by 1065

Special Issue Editor

Special Issue Information

Dear Colleagues,

Recent advances in gene editing technologies, particularly CRISPR/Cas-based systems, have revolutionized crop improvement by enabling precise genetic modifications. Cereal crops, as staple foods for the global population, present an essential target for genetic enhancement to improve yield, stress resistance, nutritional quality, and processing properties.

This Special Issue invites original research articles, reviews, and perspectives on the application of gene editing in major cereal crops such as wheat, rice, maize, and barley. Topics of interest include but are not limited to:

  • Development and optimization of gene-editing techniques for cereals;
  • Improvement of agronomic traits, including yield, stress tolerance, and disease resistance;
  • Enhancing grain quality, nutrition, and processing properties;
  • Regulatory considerations and public acceptance of gene-edited cereals;
  • Novel germplasm development and breeding applications.

We welcome contributions from researchers worldwide to showcase the latest advancements and challenges in gene editing for cereal crops. This issue aims to provide insights into how gene editing can contribute to global food security and sustainable agriculture.

Dr. Ke Wang
Guest Editor

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Keywords

  • gene-editing techniques
  • novel germplasm
  • quality
  • nutrition
  • agronomic traits

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Published Papers (1 paper)

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Review

19 pages, 1143 KB  
Review
Advances and Applications of Plant Base Editing Technologies
by Hao Peng, Jiajun Li, Kehui Sun, Huali Tang, Weihong Huang, Xi Li, Surong Wang, Ke Ding, Zhiyang Han, Zhikun Li, Le Xu and Ke Wang
Int. J. Mol. Sci. 2025, 26(19), 9452; https://doi.org/10.3390/ijms26199452 - 27 Sep 2025
Cited by 1 | Viewed by 830
Abstract
Base editing represents a major breakthrough in the field of genome editing in recent years. By fusing deaminases with the CRISPR/Cas system, it enables precise single-base modifications of DNA. This review systematically summarizes the development of base editing technologies, including cytosine base editors [...] Read more.
Base editing represents a major breakthrough in the field of genome editing in recent years. By fusing deaminases with the CRISPR/Cas system, it enables precise single-base modifications of DNA. This review systematically summarizes the development of base editing technologies, including cytosine base editors (CBEs), adenine base editors (ABEs), and glycosylase base editors (GBEs), with a particular focus on their applications in crop improvement as well as future trends and prospects. We highlight advances in the creation of novel germplasm with enhanced stress resistance and desirable agronomic traits through base editing in rice, wheat, maize, potato, and other crops, particularly for improving herbicide resistance, disease resistance, and grain quality. Furthermore, we analyze factors that influence base editing efficiency, noting that challenges remain, such as PAM sequence constraints, limited base conversion types, off-target effects, narrow editing windows, and efficiency variation. Future efforts should aim to optimize deaminase activity, expand PAM compatibility, and develop versatile tools to facilitate the broad application of base editing in molecular breeding. This review provides a timely reference for researchers and breeders, offering theoretical guidance and practical insights into harnessing base editing for crop genetic improvement. Full article
(This article belongs to the Special Issue Gene Editing for Cereal Crops)
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