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Stress Response Research: Yeast as Models: 2nd Edition
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Stress Response Research: Yeast as Models: 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 20 August 2025 | Viewed by 6994

Special Issue Editors

Dr. Cristina Mazzoni

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Guest Editor
Department of Biology and Biotechnology “C. Darwin”, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy
Interests: yeast models; ageing; cell death; oxidative stress; mRNA metabolism
Special Issues, Collections and Topics in MDPI journals
Dr. Sergio Giannattasio

E-Mail Website
Guest Editor
Institute of Biomembrane, Bioenergetics and Molecular Biotechnologies, National Research Council of Italy, Via Amendola 122/O, 70126 Bari, Italy
Interests: yeast; mitochondria; cell death; cancer; drug discovery
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is a continuation of our previous Special Issue, titled "Stress Response Research: Yeast as Models".

Saccharomyces cerevisiae yeast was among the first living beings to be domesticated, and it is used as a "cell factory" for the production of biological drugs such as insulin and other valuable molecules. It has also been used as a model to elucidate the molecular mechanisms underlying biological processes, such as the cell cycle, DNA replication, the regulation of gene expression, aging, and regulated cell death, which are crucial processes in cell stress response and the maintenance of cellular homeostasis. The elucidation of these processes is essential for understanding the molecular mechanisms underlying human disease and for biotechnological applications. This Special Issue aims to collect emerging concepts in the field of cell stress response, using yeast as a model system. The topics of interest for this Special Issue include the following: molecular pathways of cell stress response, signal transduction and protein trafficking, aging, and regulated cell death, as well as organelle biogenesis, function, and communication.

Dr. Cristina Mazzoni
Dr. Sergio Giannattasio
Guest Editors

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Keywords

  • yeast
  • stress response
  • genetic disease
  • age-related disease
  • cell factory

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Published Papers (4 papers)

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Research

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15 pages, 2386 KiB  
Article
Developing a Novel and Optimized Yeast Model for Human VDAC Research
by Martyna Baranek-Grabińska, Wojciech Grabiński, Deborah Musso, Andonis Karachitos and Hanna Kmita
Int. J. Mol. Sci. 2024, 25(23), 13010; https://doi.org/10.3390/ijms252313010 - 3 Dec 2024
Viewed by 868
Abstract
The voltage-dependent anion-selective channel (VDAC) plays a crucial role in mitochondrial function, and VDAC paralogs are considered to ensure the differential integration of mitochondrial functions with cellular activities. Heterologous expression of VDAC paralogs in the yeast Saccharomyces cerevisiae por1Δ mutant cells is often [...] Read more.
The voltage-dependent anion-selective channel (VDAC) plays a crucial role in mitochondrial function, and VDAC paralogs are considered to ensure the differential integration of mitochondrial functions with cellular activities. Heterologous expression of VDAC paralogs in the yeast Saccharomyces cerevisiae por1Δ mutant cells is often employed in studies of functional differentiation of human VDAC paralogs (hVDAC1-hVDAC3) regardless of the presence of the yeast second VDAC paralog (yVDAC2) encoded by the POR2 gene. Here, we applied por1Δpor2Δ double mutants and relevant por1Δ and por2Δ single mutants, derived from two S. cerevisiae strains (M3 and BY4741) differing distinctly in auxotrophic markers but commonly used for heterologous expression of hVDAC paralogs, to study the effect of the presence of yVDAC2 and cell genotypes including MET15, the latter resulting in a low level of hydrogen sulfide (H2S), on the complementation potential of heterologous expression of hVDAC paralogs. The results indicated that yVDAC2 might contribute to the complementation potential. Moreover, the possibility to reverse the growth phenotype through heterologous expression of hVDAC paralogs in the presence of the applied yeast cell genotype backgrounds was particularly diverse for hVDAC3 and depended on the presence of the protein cysteine residues and expression of MET15. Thus, the difference in the set of auxotrophic markers in yeast cells, including MET15 contributing to the H2S level, may create a different background for the modification of cysteine residues in hVDAC3 and thus explain the different effects of the presence and deletion of cysteine residues in hVDAC3 in M3-Δpor1Δpor2 and BY4741-Δpor1Δpor2 cells. The different phenotypes displayed by BY4741-Δpor1Δpor2 and M3-Δpor1Δpor2 cells following heterologous expression of a particular hVDAC paralog make them valuable models for the study of human VDAC proteins, especially hVDAC3, as a representative of VDAC protein sensitive to the reduction–oxidation state. Full article
(This article belongs to the Special Issue Stress Response Research: Yeast as Models: 2nd Edition)
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21 pages, 6998 KiB  
Article
Effect of Dihydroquercetin During Long-Last Growth of Yarrowia lipolytica Yeast: Anti-Aging Potential and Hormetic Properties
by Maxim S. Pusev, Olga I. Klein, Natalya N. Gessler, Galina P. Bachurina, Svetlana Yu. Filippovich, Elena P. Isakova and Yulia I. Deryabina
Int. J. Mol. Sci. 2024, 25(23), 12574; https://doi.org/10.3390/ijms252312574 - 22 Nov 2024
Viewed by 1105
Abstract
Polyphenols are powerful natural antioxidants with numerous biological activities. They change cell membrane permeability, interact with receptors, intracellular enzymes, and cell membrane transporters, and quench reactive oxygen species (ROS). Yarrowia lipolytica yeast, being similar to mammalian cells, can be used as a model [...] Read more.
Polyphenols are powerful natural antioxidants with numerous biological activities. They change cell membrane permeability, interact with receptors, intracellular enzymes, and cell membrane transporters, and quench reactive oxygen species (ROS). Yarrowia lipolytica yeast, being similar to mammalian cells, can be used as a model to study their survival ability upon long-lasting cultivation, assaying the effect of dihydroquercetin polyphenol (DHQ). The complex assessment of the physiological features of the population assaying cell respiration, survival, ROS detection, and flow cytometry was used. Y. lipolytica showed signs of chronological aging by eight weeks of growth, namely a decrease in the cell number, and size, increased ROS generation, a decrease in colony-forming unit (CFU) and metabolic activity, and decreased respiratory rate and membrane potential. An amount of 150 µM DHQ decreased ROS generation at the 6-week growth stage upon adding an oxidant of 2,2′-azobis (2-amidinopropane) dihydrochloride (AAPH). Moreover, it decreased CFU at 1–4 weeks of cultivation, inhibited cell metabolic activity of the 24-h-old culture and stimulated that on 14–56 days of growth, induced the cell respiration rate in the 24-h-old culture, and blocked alternative mitochondrial oxidase at growth late stages. DHQ serves as a mild pro-oxidant on the first day of age-stimulating anti-stress protection. In the deep stationary stage, it can act as a powerful antioxidant, stabilizing cell redox status and reducing free radical oxidation in mitochondria. It provides a stable state of population. The hormetic effects of DHQ using lower eukaryotes of Y. lipolytica have been previously discussed, which can be used as a model organism for screening geroprotective compounds of natural origin. Full article
(This article belongs to the Special Issue Stress Response Research: Yeast as Models: 2nd Edition)
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11 pages, 1220 KiB  
Article
Unraveling the Anti-Aging Properties of Phycocyanin from the Cyanobacterium Spirulina (Arthrospira platensis)
by Mariachiara Nova, Stefania Citterio, Enzo Martegani and Sonia Colombo
Int. J. Mol. Sci. 2024, 25(8), 4215; https://doi.org/10.3390/ijms25084215 - 11 Apr 2024
Cited by 6 | Viewed by 2866
Abstract
In recent years, marine natural products have become one of the most important resources of novel lead compounds for critical diseases associated with age. Spirulina, a dietary supplement made from blue-green algae (cyanobacteria: scientific name Arthrospira platensis), is particularly rich in [...] Read more.
In recent years, marine natural products have become one of the most important resources of novel lead compounds for critical diseases associated with age. Spirulina, a dietary supplement made from blue-green algae (cyanobacteria: scientific name Arthrospira platensis), is particularly rich in phycocyanin, a phycobiliprotein, which accounts for up to 20% of this cyanobacterium’s dry weight and is considered responsible for its anti-cancer, anti-inflammatory and antioxidant activities. Although the anti-aging activity of phycocyanin has been investigated, how exactly this compound works against aging remains elusive. The aim of our research is to use the yeast Saccharomyces cerevisiae as a model organism to investigate the anti-aging properties of phycocyanin from A. platensis. Our results show that phycocyanin has a powerful anti-aging effect, greatly extending the chronological life span of yeast cells in a dose-dependent way, as the effect was also pronounced when cells were grown in SD medium under calorie restriction conditions (0.2% glucose). Both ROS and accumulation of dead cells were followed by staining chronologically aged cells with dihydrorhodamine 123 (DHR123) and propidium iodide (PI). Interestingly, we found that most of the aged phycocyanin-treated cells, which were unable to form colonies, were actually ROS+/PI–. Finally, we show that the moment in which phycocyanin is added to the culture does not substantially influence its effectiveness in counteracting chronological aging. Full article
(This article belongs to the Special Issue Stress Response Research: Yeast as Models: 2nd Edition)
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Review

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12 pages, 2695 KiB  
Review
How to Survive without Water: A Short Lesson on the Desiccation Tolerance of Budding Yeast
by Zoe L. Robison, Qun Ren and Zhaojie Zhang
Int. J. Mol. Sci. 2024, 25(14), 7514; https://doi.org/10.3390/ijms25147514 - 9 Jul 2024
Cited by 1 | Viewed by 1372
Abstract
Water is essential to all life on earth. It is a major component that makes up living organisms and plays a vital role in multiple biological processes. It provides a medium for chemical and enzymatic reactions in the cell and is a major [...] Read more.
Water is essential to all life on earth. It is a major component that makes up living organisms and plays a vital role in multiple biological processes. It provides a medium for chemical and enzymatic reactions in the cell and is a major player in osmoregulation and the maintenance of cell turgidity. Despite this, many organisms, called anhydrobiotes, are capable of surviving under extremely dehydrated conditions. Less is known about how anhydrobiotes adapt and survive under desiccation stress. Studies have shown that morphological and physiological changes occur in anhydrobiotes in response to desiccation stress. Certain disaccharides and proteins, including heat shock proteins, intrinsically disordered proteins, and hydrophilins, play important roles in the desiccation tolerance of anhydrobiotes. In this review, we summarize the recent findings of desiccation tolerance in the budding yeast Saccharomyces cerevisiae. We also propose that the yeast under desiccation could be used as a model to study neurodegenerative disorders. Full article
(This article belongs to the Special Issue Stress Response Research: Yeast as Models: 2nd Edition)
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