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Molecular Insight into Oxidative Stress in Plants

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: 31 August 2024 | Viewed by 454

Special Issue Editors


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Guest Editor
Department of Plant Physiology and Biophysics, Institute of Biological Sciences, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin, Poland
Interests: abiotic and biotic stress factors; metal phytotoxicity; enzymatic and non-enzymatic antioxidants; plant-growth-promoting microorganisms; metal-contaminated soils
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Guest Editor
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Wroclaw Medical University, Borowska 211, 50-556 Wroclaw, Poland
Interests: plant biotechnology; plant in vitro cultures; plant physiology (oxidative stress, bio-elicitor response); molecular biology (gene expression level, elucidation of plant biosynthetic pathways); phytochemistry
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Under normal conditions, reactive oxygen species (ROS), such as superoxide radicals, singlet oxygen, hydrogen peroxide, and hydroxyl radicals, act as signaling molecules (oxidative signaling) in the regulation of physiological processes in plants. As a result of primary stresses (e.g., drought, frost, heat, salinity, heavy metals, organic toxins, pathogens and pests), ROS can be overproduced and accumulated in plant cells, tissues and organs. Other types of stress can cause an imbalance between the production and removal of ROS leading to the generation of secondary oxidative stress (SOS). This stress can have a destructive effect on many macromolecules within cells, including the oxidation of proteins, lipids, and nucleic acids, and can damage the structure and function of cell membranes and organelles. In plants, homeostasis is primarily achieved through cross-talk between different signaling pathways.

Under SOS conditions, there are changes in redox potential, gene expression, metabolite profile and the efficiency of biochemical pathways. Additionally, disruptions occur in the structure and activity of existing biomolecules, and defense mechanisms are activated to reduce the harmful effects of free radicals. In response to this state, the synthesis pathways of enzymatic and non-enzymatic antioxidants are activated. The activity of antioxidant enzymes, such as superoxide dismutase, catalase, peroxidase, and glutathione reductase, is particularly important. The non-enzymatic antioxidants include protective proteins (e.g., phytochelatins, metallothioneins, and defensins) and polyphenolic compounds (e.g., polyphenols, phenolic acids and flavonoids), glutathione, proline, melatonin, carotenoids, ascorbic acid and lycopene. Advanced molecular techniques are required to fully elucidate the defense mechanisms involved in SOS.

The goal of this Special Issue is to provide a deeper insight into the molecular response of plants facing SOS. Plant survival and tolerance strategies require changes in physiological, biochemical and molecular mechanisms of cellular metabolism. Application of OMICS studies like transcriptomics, metabolomics and proteomics can provide information on overexpression of antioxidant enzymes and levels of small-molecule antioxidants.

Dr. Agnieszka Hanaka
Dr. Sylwia Zielińska
Guest Editors

Manuscript Submission Information

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Keywords

  • ROS detoxification pathways
  • enzymatic and non-enzymatic antioxidants
  • SOS markers at the molecular level
  • cross-talk of ROS with phytohormones
  • restoration of plant cell homeostasis
  • mechanisms of oxidative damages and antioxidant protection
  • multiple signaling cascades in SOS prevention
  • transcriptomic analysis in gene regulatory networks
  • metabolomic and proteomic analyses

Published Papers (1 paper)

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Research

19 pages, 2623 KiB  
Article
Assessing Transcriptomic Responses to Oxidative Stress: Contrasting Wild-Type Arabidopsis Seedlings with dss1(I) and dss1(V) Gene Knockout Mutants
by Ivana Nikolić, Mira Milisavljević and Gordana Timotijević
Int. J. Mol. Sci. 2024, 25(12), 6291; https://doi.org/10.3390/ijms25126291 - 7 Jun 2024
Viewed by 267
Abstract
Oxidative stress represents a critical facet of the array of abiotic stresses affecting crop growth and yield. In this paper, we investigated the potential differences in the functions of two highly homologous Arabidopsis DSS1 proteins in terms of maintaining genome integrity and response [...] Read more.
Oxidative stress represents a critical facet of the array of abiotic stresses affecting crop growth and yield. In this paper, we investigated the potential differences in the functions of two highly homologous Arabidopsis DSS1 proteins in terms of maintaining genome integrity and response to oxidative stress. In the context of homologous recombination (HR), it was shown that overexpressing AtDSS1(I) using a functional complementation test increases the resistance of the Δdss1 mutant of Ustilago maydis to genotoxic agents. This indicates its conserved role in DNA repair via HR. To investigate the global transcriptome changes occurring in dss1 plant mutant lines, gene expression analysis was conducted using Illumina RNA sequencing technology. Individual RNA libraries were constructed from three total RNA samples isolated from dss1(I), dss1(V), and wild-type (WT) plants under hydrogen peroxide-induced stress. RNA-Seq data analysis and real-time PCR identification revealed major changes in gene expression between mutant lines and WT, while the dss1(I) and dss1(V) mutant lines exhibited analogous transcription profiles. The Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed significantly enriched metabolic pathways. Notably, genes associated with HR were upregulated in dss1 mutants compared to the WT. Otherwise, genes of the metabolic pathway responsible for the synthesis of secondary metabolites were downregulated in both dss1 mutant lines. These findings highlight the importance of understanding the molecular mechanisms of plant responses to oxidative stress. Full article
(This article belongs to the Special Issue Molecular Insight into Oxidative Stress in Plants)
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