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Molecular Mechanisms of Hemostasis, Thrombosis and Thrombo-Inflammation: 3rd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 30 August 2025 | Viewed by 598

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Special Issue Information

Dear Colleagues,

In the present decade, we are seeing a rapid increase in the available genetics and multiomics information on components of human and mammalian circulation, contributing to congenital or acquired hemostatic aberrations, athero- and venous thrombosis, thrombo-inflammation, and (acquired) immune thrombosis. With this Special Issue, we aim to collate state-of-the-art scientific contributions (reviews and original articles) that provide novel molecular insights into the complex interactions in the blood and the vessel wall in these genetic and pathophysiological settings. These include molecular mechanisms contributing to coagulation; platelet, fibrinolysis, and complement activation; leukocyte, erythrocyte, and vascular cell functions; extracellular vesicles; and intercellular crosstalk both in vitro and in vivo. Clinical papers without defined molecular mechanisms will unfortunately not be accepted.

The first edition of this Special Issue, entitled "Molecular Mechanisms of Hemostasis, Thrombosis and Thrombo-Inflammation", has seen a lot of success over the past year, with many papers both submitted and published. Because of a plethora of additional submission requests, we have thus launched the third edition of this Special Issue with MDPI.

Prof. Dr. Johan W. M. Heemskerk
Guest Editor

Manuscript Submission Information

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Keywords

  • atherosclerosis
  • coagulation
  • complement
  • endothelial cells
  • extracellular vesicles
  • fibrinolysis
  • immune thrombosis
  • inflammation
  • leukocytes
  • platelets
  • proteomics
  • red blood cells

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Published Papers (1 paper)

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Research

19 pages, 1812 KiB  
Article
Effect of Platelet-Derived Microparticles on the Expression of Adhesion Molecules in Endothelial Cells
by Elvira Varela-López, Socorro Pina-Canseco, Felipe Massó-Rojas, Claudia Lerma, Ana María Mejía Domínguez, Jesús Oswaldo García Ávila, Juan Carlos Torres-Narváez, Alvaro Vargas-González and Araceli Páez-Arenas
Int. J. Mol. Sci. 2025, 26(14), 6567; https://doi.org/10.3390/ijms26146567 - 8 Jul 2025
Viewed by 444
Abstract
In healthy conditions and cardiovascular diseases, the most abundant microparticles (MPs) in the bloodstream are those of platelet origin, but the direct effect of these microparticles on endothelial activation is poorly understood. The objective of this paper is to measure endothelial cell activation, [...] Read more.
In healthy conditions and cardiovascular diseases, the most abundant microparticles (MPs) in the bloodstream are those of platelet origin, but the direct effect of these microparticles on endothelial activation is poorly understood. The objective of this paper is to measure endothelial cell activation, as evaluated by the expression of the adhesion molecules E-selectin, VCAM-1, ICAM-1, and PECAM-1 in endothelial cell line HMEC-1 when stimulated with MPs produced by platelets stimulated in vitro with thrombin (TH), adenosine diphosphate (ADP), calcium ionophore (ICa), N-acetylglucosamine (NAcGlc), and without any stimulus. Platelets from healthy individuals induced the formation of MPs with different agonists. The results from the determination of the phenotype of the MPs showed that the expression of GPIIb/IIIa was significant, with median fold changes of TH = 2.2, ADP = 5.2, Ica = 7.0, and NAcGlc = 10.0. However, in HMEC-1 cells, the expression of adhesion molecules stimulated with MPs had a median change slightly higher for E-Sel expression (ranging from 1.4 to 4.2) and ICAM-1 expression (range 2.2 to 3.0), especially VCAM-1 expression (ranging from 15 to 18.8), all of which were significant. For PECAM-1, only stimulation with ICa (1.5) was significant, demonstrating that MPs elicit stimulus-dependent responses in endothelial cells. Platelet-derived MPs may have a potential role in modulating inflammation and other endothelial functions. Full article
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