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Biocatalysis of Compounds for Pharmaceutical and Biomedical Applications

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pharmacology".

Deadline for manuscript submissions: closed (20 June 2025) | Viewed by 2834

Special Issue Editor


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Guest Editor
Department of Medicinal Chemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, 85-089 Bydgoszcz, Poland
Interests: biocatalysis; chiral separation; enzyme; enzyme engineering; green chemistry; immobilization; kinetic resolution; support material modification
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Special Issue Information

Dear Colleagues,

Nowadays, methods using enzymes as catalysts to obtain substances of importance in the pharmaceutical and biomedical fields have become intensively researched. These biocatalytic reactions are beneficial from an ecological and economic point of view because enzyme-catalyzed reactions do not require drastic conditions, which reduces their toxicity. They also make it possible to simplify procedures by omitting, among others, many steps of enantioselective synthesis. Native (free) forms of enzymes are commonly used in biocatalytic reactions. However, it should be emphasized that an intensive study is being carried out to develop effective methods that allow for increased catalytic activity. One technique that enables the gain of higher enzymatic activity and stability is to immobilize enzymes on supports.

Dr. Tomasz Siodmiak
Guest Editor

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Keywords

  • biocatalysis
  • biomedical application
  • biotechnology
  • compounds for pharmacy
  • compounds for biomedicine
  • enantiomers
  • enzyme activity
  • enzyme engineering
  • enzyme immobilization
  • kinetic resolution
  • pharmaceutical application
  • supports materials

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Published Papers (2 papers)

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14 pages, 2686 KiB  
Article
Enzymatic Kinetic Resolution of Racemic 1-(Isopropylamine)-3-phenoxy-2-propanol: A Building Block for β-Blockers
by Joanna Chałupka, Michał Piotr Marszałł and Adam Sikora
Int. J. Mol. Sci. 2024, 25(19), 10730; https://doi.org/10.3390/ijms251910730 - 5 Oct 2024
Cited by 1 | Viewed by 1070
Abstract
This study aimed to optimize the kinetic resolution of building blocks for the synthesis of β-blockers using Candida rugosa lipases, which could be potentially used to synthesize enantiomerically pure β-blockers further. Reaction mixtures were incubated in a thermostated shaker. Qualitative and quantitative analyses [...] Read more.
This study aimed to optimize the kinetic resolution of building blocks for the synthesis of β-blockers using Candida rugosa lipases, which could be potentially used to synthesize enantiomerically pure β-blockers further. Reaction mixtures were incubated in a thermostated shaker. Qualitative and quantitative analyses of the reaction mixtures were performed using chiral stationary phases and the UPLC-IT-TOF system. Of the 24 catalytic systems prepared, a system containing lipase from Candida rugosa MY, [EMIM][BF4] and toluene as a two-phase reaction medium and isopropenyl acetate as an acetylating agent was optimal. This resulted in a product with high enantiomeric purity produced via biotransformation, whose enantioselectivity was E = 67.5. Using lipases from Candida rugosa enables the enantioselective biotransformation of the β-blockers building block. The biocatalyst used, the reaction environment, and the acetylating agent significantly influence the efficiency of performer kinetic resolutions. The studies made it possible to select an optimum system, a prerequisite for obtaining a product of high enantiomeric purity. As a result of the performed biotransformation, the (S)-enantiomer of the β-blocker derivative was obtained, which can be used to further synthesize enantiomerically pure β-blockers. Full article
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Review

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17 pages, 1759 KiB  
Review
Progress in Methylxanthine Biosynthesis: Insights into Pathways and Engineering Strategies
by Tongtong Jiang, Shangci Zuo, Chang Liu, Wanbin Xing and Pengchao Wang
Int. J. Mol. Sci. 2025, 26(4), 1510; https://doi.org/10.3390/ijms26041510 - 11 Feb 2025
Viewed by 1248
Abstract
Methylxanthines are ubiquitous purine alkaloids in nature and have rich biological activities and functions. Today, the demand for methylxanthine is increasing but its production is low. This issue prevents its widespread use in many industrial fields, such as pharmaceuticals, food manufacturing, and chemical [...] Read more.
Methylxanthines are ubiquitous purine alkaloids in nature and have rich biological activities and functions. Today, the demand for methylxanthine is increasing but its production is low. This issue prevents its widespread use in many industrial fields, such as pharmaceuticals, food manufacturing, and chemical engineering. To address these issues, this review provides a comprehensive and systematic exploration of methylxanthines, delving into their biological structures, detailed biosynthetic pathways, and the latest research trends. These findings serve as valuable references for researchers, fostering advancements in the optimization of synthesis processes for methylxanthines and their derivatives and promoting their application across diverse industrial fields, such as medicine, food, and chemical engineering. By bridging fundamental research and practical applications, this work aims to advance the understanding of methylxanthine compounds, enhance their production efficiency, and contribute to healthcare and technological progress. Full article
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