ijms-logo

Journal Browser

Journal Browser

Photophysics and Photochemistry in Biological Molecules

A Special Issue of International Journal of Molecular Sciences (ISSN 1422-0067) belonging to the section "Biochemistry".

Deadline for manuscript submissions: 31 March 2027 | Viewed by 1482

Editor


E-Mail Website
Guest Editor
Instituto para el Desarrollo Agroindustrial y de la Salud, Universidad Nacional de Río Cuarto (UNRC) and the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Río Cuarto, Córdoba, Argentina
Interests: UVB light; biodegradation; effect of sunlight on biomolecules

Special Issue Information

Dear Colleagues,

The field of photochemistry and photophysics is very broad and includes, for example, kinetic and mechanistic studies of various molecules. Among the many processes that fall under this research area, one such example is photodegradation processes, which can occur through direct or sensitized photolysis. The latter requires a substance called a sensitizer that initiates the reaction and generates reactive oxygen species with the molecule in question. These processes are very useful for degrading, for example, compounds called emerging contaminants, which are not completely removed in wastewater treatment plants. In this particular Special Issue, the focus is on the study of biomolecules, among which the most important are carbohydrates, lipids, proteins, and nucleic acids. Among the biomolecules mentioned, it is worth highlighting the relevance of biological remediation processes using enzymes, which are an effective, sustainable, and environmentally friendly alternative for the removal of contaminants.

Prof. Dr. María Alicia Biasutti
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • photochemistry
  • photodegradation
  • Reactive Oxygen Species (ROS)
  • emerging contaminants
  • bioremediation
  • biomolecules
  • carotenoids
  • enzymes
  • fatty acids
  • DNA
  • RNA
  • photodynamic therapy
  • fluorescence
  • photosynthesis
  • phototoxicity
  • photosensitizers

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

22 pages, 7616 KB  
Article
Individual and Cooperative Photochemical–Enzymatic Processes for the Degradation of the Dye Bromothymol Blue: Kinetic and Eco-Toxicity Analysis
by Andrea S. Urquiza, Agustina Reynoso, M. Alicia Biasutti, Hernán A. Montejano and Eugenia Reynoso
Int. J. Mol. Sci. 2026, 27(17), 7767; https://doi.org/10.3390/ijms27177767 - 30 Aug 2026
Viewed by 233
Abstract
The degradation of bromothymol blue (BTB) in aqueous solution was investigated through individual and sequential photochemical and enzymatic treatments. Photodegradation experiments were performed under UVC, UVB, UVA and visible irradiation at different pH values and atmosphere conditions, while enzymatic degradation was evaluated using [...] Read more.
The degradation of bromothymol blue (BTB) in aqueous solution was investigated through individual and sequential photochemical and enzymatic treatments. Photodegradation experiments were performed under UVC, UVB, UVA and visible irradiation at different pH values and atmosphere conditions, while enzymatic degradation was evaluated using Laccase from Trametes versicolor under varying pH, temperature and enzyme concentration. Kinetic analyses were performed in all cases. BTB degradation was strongly dependent on irradiation wavelength and pH. The highest photodegradation rates were obtained under UVC irradiation, particularly in alkaline medium. Additionally, our results suggest that BTB photolysis mainly proceeds through a unimolecular pathway. On the other hand, enzymatic degradation was favored at acidic pH, elevated temperature, and high amount of laccase, with optimal performance observed at pH 5 and 40 °C. Sequential treatments combining photochemical and enzymatic processes improved the overall removal efficiency, reaching degradation values above 70% regardless of the treatments order. Ecotoxicological evaluation using the Vibrio fischeri bioluminescence inhibition assay revealed a significant reduction in toxicity after all treatments, particularly those involving UVC irradiation. These results demonstrate the complementary nature of both processes and highlight the potential of combined photochemical–enzymatic treatments for the remediation of dye-contaminated waters. Full article
(This article belongs to the Special Issue Photophysics and Photochemistry in Biological Molecules)
Show Figures

Figure 1

15 pages, 1914 KB  
Article
Genetic Encoding of 3-Cyano-Tyrosine and Its Use in Controlling the Chromophore Isomeric State of the Fluorescent Protein mKate
by Connor J. Stevenson, John J. K. McLarnon, James Harnedy, Salma A. Elsherbeni, Debarshi Saha, Wolfgang Langbein, Paola Borri, Jamie A. Platts, Louis C. Morril and D. Dafydd Jones
Int. J. Mol. Sci. 2026, 27(16), 7184; https://doi.org/10.3390/ijms27167184 - 11 Aug 2026
Viewed by 597
Abstract
Switchable β-barrel-type fluorescent proteins are essential genetically encoded probes for super-resolution imaging. The space required for chromophore cis–trans isomerisation can also provide an opportunity to introduce bulkier chemistry at the 3-position of the phenolic ring. Here, we report, to our knowledge, the first [...] Read more.
Switchable β-barrel-type fluorescent proteins are essential genetically encoded probes for super-resolution imaging. The space required for chromophore cis–trans isomerisation can also provide an opportunity to introduce bulkier chemistry at the 3-position of the phenolic ring. Here, we report, to our knowledge, the first successful genetic encoding of 3-cyano-L-tyrosine (3CNY) into a protein. Using genetic code expansion, the cyano-containing tyrosine derivative is incorporated directly into the chromophore of mKate, a pH-dependent switchable red fluorescent protein. While mKate adopts a fluorescent phenolate cis-state chromophore at physiological pH, substituting the native tyrosine with 3CNY yields a functional protein exhibiting hypsochromically shifted spectral properties. Time-dependent density functional theory (TD-DFT) calculations indicate that 3CNY incorporation results in a trans state at pH 8 but, unlike mKate, is fluorescent. The electron-withdrawing cyano group potentially perturbs conjugation across the chromophore, thus lowering the barrier to cis–trans isomerisation. The trans form may also be stabilised by hydrogen bonds from the cyano group to the rest of the protein. Overall, the introduction of a genetically encoded 3-CNY tyrosine analogue into a fluorescent protein chromophore expands our mechanistic understanding and enables the incorporation of a new chemical tag directly into the chromophore. Full article
(This article belongs to the Special Issue Photophysics and Photochemistry in Biological Molecules)
Show Figures

Figure 1

Back to TopTop