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Post-Transcriptional Gene Regulation of mRNA and Protein Expression

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: 31 July 2026 | Viewed by 646

Special Issue Editor


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Guest Editor
1. Laboratory of mRNA & Cancer, Unit of Biomedical Research on Cancer, National Institute of Cancer (Instituto Nacional de Cancerología, INCan), Mexico City 14080, Mexico
2. School of Medicine and Health Sciences, Tecnológico de Monterrey, Mexico City 14380, Mexico
Interests: translational control; mRNA biochemistry; gene expression; developmental biology; molecular biology; cancer
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Special Issue Information

Dear Colleagues,

Post-transcriptional regulation of gene expression is a series of processes crucial to define the composition of the proteome in all forms of life and diseases. Dysregulation of these processes has emerged as a crucial cause of many diseases. Moreover, malfunctioning of the signal transduction cascades targeting post-transcriptional regulation of gene expression is also involved in the onset and progression of diverse phenotypes. Thus, research for the use and development of different pharmacological compounds targeting these processes of gene expression has become paramount to treat various diseases.

We dedicate this Special Issue to discussing the state-of-the-art of basic and clinical aspects of post-transcriptional regulation of gene expression. Original research articles and reviews are welcome. Research areas may include (but are not limited to) the following:

  • mRNA maturation;
  • mRNA editing;
  • mRNA export;
  • mRNA storage;
  • mRNA turnover;
  • Translational control;
  • Translation machinery;
  • Signal transduction;
  • Pharmacological research targeting cell machineries of post-transcriptional control;
  • Clinical research on post-transcription-related diseases.

I thank the enthusiastic and excellent labor of all contributors and of the editorial team to make this collection on Post-Transcriptional Gene Regulation of mRNA and Protein Expression possible.

I look forward to receiving your contributions.

Dr. Greco Hernández
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • translational control
  • protein synthesis
  • mRNA biochemistry
  • signal transduction
  • mTOR pathway
  • post-transcriptional regulation

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Published Papers (1 paper)

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Research

27 pages, 10699 KB  
Article
Eukaryotic Initiation Factor 3F (eIF3F) Regulates the IRES-Mediated Translation of Bcl-xL via Its Interaction with Programmed Cell Death 4 (PDCD4) Protein
by Veda Hegde, Divya K. Sharma, Harshil Patel, Pavan Lakshmi Narasimha, Jason Luddu, Rebecca Mubaya, Martin Holcik and Nehal Thakor
Int. J. Mol. Sci. 2026, 27(9), 3955; https://doi.org/10.3390/ijms27093955 - 29 Apr 2026
Viewed by 410
Abstract
Programmed cell death 4 (PDCD4) protein is a tumour suppressor protein that inhibits mRNA translation by inhibiting RNA helicase, eukaryotic initiation factor 4A (eIF4A). We have previously reported that PDCD4 interacts with the internal ribosome entry site (IRES) element of B-cell lymphoma extra-large [...] Read more.
Programmed cell death 4 (PDCD4) protein is a tumour suppressor protein that inhibits mRNA translation by inhibiting RNA helicase, eukaryotic initiation factor 4A (eIF4A). We have previously reported that PDCD4 interacts with the internal ribosome entry site (IRES) element of B-cell lymphoma extra-large (Bcl-xL) mRNA and inhibits its IRES-mediated translation initiation. S6 kinase (S6K)-mediated phosphorylation of PDCD4 activates its degradation and derepresses IRES-mediated translation initiation of Bcl-xL mRNA. eIF3F (one of the subunits of eIF3 complex) was reported to recruit S6K to phosphorylate eIF3G. Therefore, we investigated the possibility of co-regulation of PDCD4 and eIF3F by S6K and the regulation of IRES-mediated translation initiation by PDCD4–eIF3F. Here, we demonstrated that PDCD4 interacts with several subunits of eIF3. Specifically, eIF3F directly interacts with PDCD4 in an RNA-independent manner. Depletion of PDCD4 in glioblastoma (GBM) cells resulted in decreased levels of certain eIF3 subunits, including eIF3F. Additionally, depletion of eIF3F from GBM cells decreased the levels of PDCD4 protein. We also showed that PDCD4 and eIF3F directly interact with Bcl-xL RNA independently of each other. By performing IRES reporter, polysome profiling assays and EMSA we have demonstrated that eIF3F regulates IRES-mediated translation of Bcl-xL mRNA, likely via its interaction with PDCD4. Full article
(This article belongs to the Special Issue Post-Transcriptional Gene Regulation of mRNA and Protein Expression)
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