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Advanced Research on Malaria: Molecular and Biochemical Perspectives

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: closed (20 April 2026) | Viewed by 1033

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Guest Editor
Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal
Interests: genome; malaria; parasites; resistance; adaptation
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Special Issue Information

Dear Colleagues,

Malaria remains one of the most significant global health challenges, claiming hundreds of thousands of lives annually, particularly in low- and middle-income countries. Despite decades of research, the parasite’s complex biology, drug resistance, and host-pathogen dynamics pose ongoing challenges as previously implemented interventions are not performing as expected. To accelerate the fight against malaria, we propose a special issue dedicated to exploring its molecular and biochemical dimensions. This issue will aim to bridge gaps in understanding the parasite’s biology, immune evasion strategies, and potential therapeutic avenues, providing a platform for groundbreaking research and innovative solutions.

Aims and Scope: The proposed special issue will focus on the latest advances in molecular and biochemical research on malaria.

The primary aims are:

  • To deepen our understanding of Plasmodium biology, including its genetic, metabolic, and biochemical adaptations.
  • To highlight host-pathogen interactions, particularly immune response mechanisms and their implications for disease progression.
  • To showcase novel therapeutic targets and diagnostic tools derived from molecular and biochemical studies.
  • To foster multidisciplinary collaborations by integrating findings from computational biology, structural biology, and systems biology.

The special issue will be organized around the following thematic areas:

  1. Molecular Mechanisms of Pathogenesis
  2. Host-Parasite Interactions
  3. Biochemical Pathways and Therapeutic Development
  4. Emerging Diagnostics and Vaccines
  5. Multidisciplinary Approaches and Systems Biology

Dr. Pedro Eduardo Ferreira
Guest Editor

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • malaria
  • vectors
  • therapies
  • resistance
  • cellular mechanisms

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Published Papers (1 paper)

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Research

11 pages, 1092 KB  
Article
Profiling 26S Proteasome Activity of Plasmodium falciparum Monitored by a Live-Cell Assay
by Adriana F. Gonçalves, Ana Lima-Pinheiro, Belém Sampaio-Marques and Pedro E. Ferreira
Int. J. Mol. Sci. 2026, 27(5), 2104; https://doi.org/10.3390/ijms27052104 - 24 Feb 2026
Viewed by 511
Abstract
Malaria remains a major global health challenge, driven in part by widespread antimalarial drug resistance in Plasmodium parasites. Artemisinin-based combination therapies (ACTs) are currently the first-line treatment; however, resistance has also emerged. Artemisinin damages parasite proteins, promoting their ubiquitination and subsequent proteasomal degradation. [...] Read more.
Malaria remains a major global health challenge, driven in part by widespread antimalarial drug resistance in Plasmodium parasites. Artemisinin-based combination therapies (ACTs) are currently the first-line treatment; however, resistance has also emerged. Artemisinin damages parasite proteins, promoting their ubiquitination and subsequent proteasomal degradation. Because inhibitors of the Plasmodium 26S proteasome synergize with artemisinin, the proteasome has emerged as a promising drug target, yet tools to monitor its function in live parasites remain limited. Here, we generated a P. falciparum line expressing green fluorescent protein fused to a destabilization domain (GFP-DD) to assess proteasome activity and combined it with MitoTrackerTM staining. In the absence of the stabilizing ligand Shield-1, the GFP-DD reporter is rapidly degraded by the proteasome. Using fluorescence microscopy and flow cytometry, we show that GFP-DD fluorescence provides a quantitative, inverse readout of proteasomal activity, increasing upon ligand-mediated stabilization or pharmacological inhibition with MG132. Shield-1 titration identified an optimal stabilization range, and MG132 induced a dose-dependent fluorescence increase. This work establishes a practical live-cell platform to probe ubiquitin–proteasome system function, with potential applications in future phenotypic screening and antimalarial resistance studies. Full article
(This article belongs to the Special Issue Advanced Research on Malaria: Molecular and Biochemical Perspectives)
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