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3D Technology in Biomedicine: Studying Molecular Mechanisms in Health and Disease

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Materials Science".

Deadline for manuscript submissions: 20 September 2025 | Viewed by 407

Special Issue Editor


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Guest Editor
Department of Cellular, Computational and Integrative Biology (CIBIO), Centre for Medical Sciences (CISMED), University of Trento, 38123 Trento, Italy
Interests: nanoparticles; regenerative medicine; small molecules; tissue engineering; 3D technology; muscle dystrophy

Special Issue Information

Dear Colleagues,

Medical research has exploited 3D models to investigate preventive and reparative strategies for several human diseases. These in vitro models have the advantage of serving as reliable biomimetic systems, thus reducing animal use and the often hardly available human explants.

Different 3D technologies (spheroids and organoids, bioprinted tissue models, skin equivalent, organ-on-a-chip, etc.)  have been set up by researchers to investigate the molecular mechanisms in healthy, diseased, and regenerating tissues.

In this scenario, the present Special Issue is aimed at illustrating the contribution of 3D technology in biomedical research.

As guest editors, we encourage researchers from the academy and industry to present their results in reviews or original articles: this will highlight the current state-of-the-art and innovative strategies in the application of 3D models to elucidate the molecular underpinnings of biological phenomena in health and disease.

Dr. Flavia Carton
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • 3D technologies
  • biomedicine
  • molecular biology
  • in vitro/ex vivo models
  • regenerative medicine
  • human diseases

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Published Papers (1 paper)

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Research

16 pages, 4557 KiB  
Article
A Fluid Dynamic In Vitro System to Study the Effect of Hyaluronic Acid Administration on Collagen Organization in Human Skin Explants
by Andrea Galvan, Maria Assunta Lacavalla, Federico Boschi, Barbara Cisterna, Edoardo Dalla Pozza, Enrico Vigato, Flavia Carton, Manuela Malatesta and Laura Calderan
Int. J. Mol. Sci. 2025, 26(11), 5397; https://doi.org/10.3390/ijms26115397 - 4 Jun 2025
Viewed by 13
Abstract
Hyaluronic acid (HA) is an unbranched polysaccharide particularly abundant in the extracellular matrix (ECM) of soft connective tissues. In humans, about 50% of the total HA in the organism is localized in the skin. HA plays an essential role in the hydration of [...] Read more.
Hyaluronic acid (HA) is an unbranched polysaccharide particularly abundant in the extracellular matrix (ECM) of soft connective tissues. In humans, about 50% of the total HA in the organism is localized in the skin. HA plays an essential role in the hydration of the ECM, in the regulation of tissue homeostasis, in the resistance to mechanical stimuli/forces, and in the modulation of tissue regeneration. For these reasons, HA is widely used in regenerative medicine and cosmetics. In this study we used an innovative fluid dynamic system to investigate the effects of a cross-linked macrostructural HA formulation on dermal collagen of healthy human skin explants. The good preservation of skin explants provided by the bioreactor allowed applying refined high-resolution microscopy techniques to analyze in situ the HA-induced modifications on the ECM collagen fibrils up to 48 h from the application on the skin surface. Results demonstrated that this HA formulation, commercially proposed for subcutaneous injection, may act on dermal ECM also when applied transcutaneously, improving ECM hydration and modifying the organization of the collagen fibrils. These findings, obtained by the original combination of explanted human skin use with an advanced culture system and multiscale imaging techniques, are consistent with the volumizing and anti-aging effect of HA. Full article
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