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Upcycling Food Industry By-Products: Bioactive Ingredients for Nutrition and Health

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Bioactives and Nutraceuticals".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 981

Special Issue Editors


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Guest Editor
Department of Bioscience, Biotechnology and Environment, University of Bari Aldo Moro, Via Orabona 4, 70125 Bari, Italy
Interests: agri-food waste valorization; bioactive compounds; biological activity; by-products; food biotechnology; food microbiology and safety; food & nutrition; functional food; gastrointestinal digestion; gut microbiota; nutraceuticals; nutritional characterization; polyphenols; probiotics

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Guest Editor
Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy
Interests: by-products upcycling for food industry; green chemistry; food & nutrition; confocal microscopy; biochemistry; analytical chemistry; antioxidants; mass spectrometry; food science and technology; super-critical carbon dioxide
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In the context of sustainability and molecular nutrition, the valorization of food industry by-products represents a valuable strategy to recover bioactive compounds and develop functional ingredients with potential health benefits. This Special Issue, entitled “Upcycling Food Industry By-Products: Bioactive Ingredients for Nutrition and Health”, will focus on the nutritional and molecular characterization, extraction, and biological activity of bioactive compounds obtained from agro-industrial residues and other natural sources. We invite original research articles and reviews exploring the structure, composition, and functional properties of bioactive compounds such as polyphenols, peptides, vitamins, fibers, and proteins, with emphasis on their bioaccessibility, bioavailability, and molecular mechanisms of action, particularly antioxidant, anti-inflammatory, immunomodulatory, and metabolic effects. We encourage contributions highlighting molecular biology, cellular, and biochemical methods used to elucidate these mechanisms, as well as biotechnological innovations for the sustainable extraction and stabilization of such compounds. Studies addressing the interaction between bioactives and molecular targets related to inflammation, oxidative stress, and metabolic disorders are welcome. The Special Issue also aims to explore the molecular basis of prebiotic and postbiotic effects, along with nutrigenomic approaches and microbiome modulation for personalized nutrition. While it emphasizes compounds derived from food by-products, submissions involving bioactive compounds from any well-defined natural source are also suitable, provided they include a clear nutritional and molecular focus.

Dr. Giusy Rita Caponio
Dr. Graziana Difonzo
Guest Editors

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Keywords

  • bioactive compounds
  • food by-products
  • functional foods
  • molecular mechanisms
  • polyphenols
  • oxidative stress
  • inflammation
  • nutrigenomics
  • microbiome
  • prebiotics
  • postbiotics

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Published Papers (1 paper)

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Research

18 pages, 1035 KB  
Article
Enzymatic Hydrolysis of Porcine Blood as a Strategy to Obtain a Peptide-Rich Functional Ingredient
by Cristina Moreno-Mariscal, Federico Moroni, Jaume Pérez-Sánchez, Leticia Mora and Fidel Toldrá
Int. J. Mol. Sci. 2025, 26(20), 9863; https://doi.org/10.3390/ijms26209863 - 10 Oct 2025
Cited by 1 | Viewed by 793
Abstract
The sustainable revalorization of porcine blood is crucial due to the large volumes daily generated in slaughterhouses. The aim of this study was to obtain a novel ingredient rich in free amino acids and bioactive peptides from the sequential hydrolysis of porcine blood. [...] Read more.
The sustainable revalorization of porcine blood is crucial due to the large volumes daily generated in slaughterhouses. The aim of this study was to obtain a novel ingredient rich in free amino acids and bioactive peptides from the sequential hydrolysis of porcine blood. Porcine blood was hydrolyzed with Alcalase 4.0 L and Protana™ Prime enzymes, followed by molecular weight fractionation (<10 kDa) and spray-drying. The antioxidant, hypoglycemic, and anti-inflammatory bioactivities of the resulting hydrolysate (PBSH) were studied in vitro. Further fractionation by reversed-phase high-performance liquid chromatography (RP-HPLC) was performed to isolate the most bioactive fraction based on polarity. Peptides from fraction 1 (F1) were identified using LC-MS/MS and analyzed in silico. Finally, some peptides were synthesized, and their bioactivity was subsequently assessed. PBSH hydrolysate showed antioxidant activity with IC50 values of 2.09, 135.05, and 26.73 mg/mL for ABTS, FRAP, and DPPH assays, respectively. Additionally, PBSH exhibited hypoglycemic, anti-inflammatory, and immunomodulatory potential through the inhibition of DPP-IV (82.78%), NEP (84.72%), TACE (50.79%), and MGL (69.08%) enzymes at a concentration of 20, 20, 100, and 20 mg/mL, respectively. Peptides PDDFNPS, FPPKPKD, DNPIPK, GHLDDLPG, and GDL were identified in the most polar and bioactive fraction (F1) and proved a synergistic hypoglycemic effect at a concentration of 1 mmol/L. The peptide PDDFNPS exhibited multifunctional properties with 56.43% inhibition of DPP-IV and 83.54% inhibition of NEP. PBSH resulted in a novel functional ingredient for animal feed as it contains a variety of identified bioactive peptides and a high amount of free amino acids. Full article
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