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Regulation of Plant Protein Homeostasis under Stress

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: closed (20 December 2023) | Viewed by 4552

Special Issue Editors


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Guest Editor
Instituto de Biología Molecular y Celular de Plantas, Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia, 46022 Valencia, Spain
Interests: plant plasticity; stress signaling; hormonal integration
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
Instituto de Biología Molecular y Celular de Plantas, CSIC/Universidad Politécnica de Valencia, Ingeniero Fausto Elio, s/n. 46022 Valencia, Spain
Interests: polyamines; thermospermine; spermidine; eIF5A; hypusination; translation factors; abiotic stress; cell death; autophagy
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The expectation in the coming decades of an increase in the global population together with severe climate restrictions poses high risk to food and feed security in large areas of our planet. A profound change in food production and agriculture is required in order to nourish the current 815 million hungry people today and the additional 2 billion people expected by 2050. On the other hand, consequences of climate change are affecting national economies with more frequent and numerous extreme weather events and new records of greenhouse gas emissions. In this context, plant biotechnology may provide solutions to agricultural practices by enhancing plant yield production and tolerance to adverse growth conditions caused by either biotic or abiotic challenges or their simultaneous appearance.

However, any approach for biotechnological intervention to improve plant stress tolerance demands a deep knowledge of the underlying molecular mechanisms involved in the plant response. Plants, as sessile organisms, have acquired numerous mechanisms to cope with stress conditions. This eventually converges on the regulation of protein homeostasis, namely the complete set of molecular events which ultimately define the protein level, localization and activity of the whole proteome. Among other processes we include protein biosynthesis, protein transport, protein modifications, protein interactions and protein targeted degradation.

In this Special Issue on the ‘Regulation of Plant Protein Homeostasis Under Stress’, we invite authors to contribute either original research or review studies to understand the relevance of protein homeostasis to plant stress adaptation, including biotic and/or abiotic insults affecting plant growth, development and yield.

Dr. Borja Belda-Palazón
Dr. Alejandro Ferrando
Guest Editors

Manuscript Submission Information

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Keywords

  • protein biosynthesis
  • protein transport
  • protein modifications
  • protein interactions
  • protein-targeted degradation

Published Papers (3 papers)

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15 pages, 772 KiB  
Review
Mechanisms of Endoplasmic Reticulum Protein Homeostasis in Plants
by Zhihao Duan, Kai Chen, Tao Yang, Ronghui You, Binzhao Chen, Jianming Li and Linchuan Liu
Int. J. Mol. Sci. 2023, 24(24), 17599; https://doi.org/10.3390/ijms242417599 - 18 Dec 2023
Cited by 1 | Viewed by 1456
Abstract
Maintenance of proteome integrity is essential for cell function and survival in changing cellular and environmental conditions. The endoplasmic reticulum (ER) is the major site for the synthesis of secretory and membrane proteins. However, the accumulation of unfolded or misfolded proteins can perturb [...] Read more.
Maintenance of proteome integrity is essential for cell function and survival in changing cellular and environmental conditions. The endoplasmic reticulum (ER) is the major site for the synthesis of secretory and membrane proteins. However, the accumulation of unfolded or misfolded proteins can perturb ER protein homeostasis, leading to ER stress and compromising cellular function. Eukaryotic organisms have evolved sophisticated and conserved protein quality control systems to ensure protein folding fidelity via the unfolded protein response (UPR) and to eliminate potentially harmful proteins via ER-associated degradation (ERAD) and ER-phagy. In this review, we summarize recent advances in our understanding of the mechanisms of ER protein homeostasis in plants and discuss the crosstalk between different quality control systems. Finally, we will address unanswered questions in this field. Full article
(This article belongs to the Special Issue Regulation of Plant Protein Homeostasis under Stress)
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13 pages, 1910 KiB  
Review
The Great Game between Plants and Viruses: A Focus on Protein Homeostasis
by Hangjun Sun, Xinxin Jing, Chaonan Wang, Pengyue Wang, Ziting Huang, Bingjian Sun, Pengbai Li, Honglian Li and Chao Zhang
Int. J. Mol. Sci. 2023, 24(16), 12582; https://doi.org/10.3390/ijms241612582 - 9 Aug 2023
Viewed by 1304
Abstract
Plant viruses are tiny pathogenic obligate parasites that cause significant damage to global crop production. They exploit and manipulate the cellular components of host plants to ensure their own survival. In response, plants activate multiple defense signaling pathways, such as gene silencing and [...] Read more.
Plant viruses are tiny pathogenic obligate parasites that cause significant damage to global crop production. They exploit and manipulate the cellular components of host plants to ensure their own survival. In response, plants activate multiple defense signaling pathways, such as gene silencing and plant hormone signaling, to hinder virus propagation. Growing evidence suggests that the regulation of protein homeostasis plays a vital role in the ongoing battle between plants and viruses. The ubiquitin-proteasome-degradation system (UPS) and autophagy, as two major protein-degradation pathways, are widely utilized by plants and viruses in their arms race. One the one hand, these pathways act as essential components of plant’s antiviral defense system by facilitating the degradation of viral proteins; on the other hand, viruses exploit the UPS and autophagy to create a favorable intracellular environment for viral infection. This review aims to provide a comprehensive summary of the events involved in protein homeostasis regulation during viral infection in plants. Gaining knowledge in this area will enhance our understanding of the complex interplay between plants and viruses. Full article
(This article belongs to the Special Issue Regulation of Plant Protein Homeostasis under Stress)
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15 pages, 1857 KiB  
Article
An Escherichia coli-Based Phosphorylation System for Efficient Screening of Kinase Substrates
by Andrés Cayuela, Adela Villasante-Fernández, Antonio Corbalán-Acedo, Elena Baena-González, Alejandro Ferrando and Borja Belda-Palazón
Int. J. Mol. Sci. 2024, 25(7), 3813; https://doi.org/10.3390/ijms25073813 - 29 Mar 2024
Viewed by 1084
Abstract
Posttranslational modifications (PTMs), particularly phosphorylation, play a pivotal role in expanding the complexity of the proteome and regulating diverse cellular processes. In this study, we present an efficient Escherichia coli phosphorylation system designed to streamline the evaluation of potential substrates for Arabidopsis thaliana [...] Read more.
Posttranslational modifications (PTMs), particularly phosphorylation, play a pivotal role in expanding the complexity of the proteome and regulating diverse cellular processes. In this study, we present an efficient Escherichia coli phosphorylation system designed to streamline the evaluation of potential substrates for Arabidopsis thaliana plant kinases, although the technology is amenable to any. The methodology involves the use of IPTG-inducible vectors for co-expressing kinases and substrates, eliminating the need for radioactive isotopes and prior protein purification. We validated the system’s efficacy by assessing the phosphorylation of well-established substrates of the plant kinase SnRK1, including the rat ACETYL-COA CARBOXYLASE 1 (ACC1) and FYVE1/FREE1 proteins. The results demonstrated the specificity and reliability of the system in studying kinase-substrate interactions. Furthermore, we applied the system to investigate the phosphorylation cascade involving the A. thaliana MKK3-MPK2 kinase module. The activation of MPK2 by MKK3 was demonstrated to phosphorylate the Myelin Basic Protein (MBP), confirming the system’s ability to unravel sequential enzymatic steps in phosphorylation cascades. Overall, this E. coli phosphorylation system offers a rapid, cost-effective, and reliable approach for screening potential kinase substrates, presenting a valuable tool to complement the current portfolio of molecular techniques for advancing our understanding of kinase functions and their roles in cellular signaling pathways. Full article
(This article belongs to the Special Issue Regulation of Plant Protein Homeostasis under Stress)
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