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Recent Research on Natural Compounds: Biosynthesis, Analysis, and Application

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 31 October 2026 | Viewed by 794

Editor


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Guest Editor
Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Sofia, 1000 Sofia, Bulgaria
Interests: phytochemistry; chromatography; mass spectrometry; GC-MS; analysis; biologically active substances of natural origin; pharmacognosy
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Special Issue Information

Dear Colleagues,

The interest in natural compounds and their activities drives research through extensive and multidisciplinary pathways. Identifying the compound responsible for an observed action is is far from straightforward. In addition, advances in analytical methods allow the precise molecular mechanism to be studied. Novel sources of biologically active compounds continue to emerge. Establishing these in practice requires not only knowledge of the chemical composition but also a deep understanding of the underlying molecular mechanism involved in the effect. On the other hand, understanding their biosynthesis is important in aspects of activity-driven production.

Topics of interest include, but are not limited to, the identification and quantification of natural compounds (with modern or novel methods), biosynthesis, possible application (pharmaceutical or cosmetic), and biological activity. Since IJMS is a journal of molecular science, clinical or purely model studies are not suitable, except if they are also supported by biomolecular experiments.

Dr. Aleksandar Shkondrov
Guest Editor

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Keywords

  • phytochemicals
  • natural compounds
  • chromatography
  • MS
  • NMR
  • analysis
  • identification
  • extraction
  • standardisation
  • quantitation
  • activity
  • moleabular mechanism
  • biosynthesis

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Published Papers (1 paper)

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Research

22 pages, 2837 KB  
Article
Unveiling Pulmonaria rubra Schott: Phytochemical Characterisation and Evaluation of Its Neuroprotective Potential
by Ivan Stambolov, Aleksandar Shkondrov, Lyubomira Vusheva, Magdalena Kondeva-Burdina and Ilina Krasteva
Int. J. Mol. Sci. 2026, 27(14), 6122; https://doi.org/10.3390/ijms27146122 - 8 Jul 2026
Viewed by 361
Abstract
Pulmonaria rubra (Boraginaceae) is a widely distributed plant in Bulgaria, yet its phytochemical profile and therapeutic potential have remained unexplored. P. rubra methanol extract (PRE) was evaluated through phytochemical profiling and in vitro neuroprotective and antioxidant assays. Rat brain synaptosomes, mitochondria and microsomes [...] Read more.
Pulmonaria rubra (Boraginaceae) is a widely distributed plant in Bulgaria, yet its phytochemical profile and therapeutic potential have remained unexplored. P. rubra methanol extract (PRE) was evaluated through phytochemical profiling and in vitro neuroprotective and antioxidant assays. Rat brain synaptosomes, mitochondria and microsomes were treated with PRE alone, and in combination with 6-hydroxydopamine and tert-butyl hydroperoxide as toxic agents. The extract exhibited concentration-dependent protective effects in all subcellular models. Additionally, it was tested on hMAOA/B and different isoforms of CYP450 enzymes, but it did not show any activity in the tested conditions. In the UHPLC-HRESIMS analysis, 26 secondary metabolites were identified, mainly hydroxycinnamic acids and caffeoyl oligomers, flavonoids, a lignan (globoidnan A), and the terpenoid glycoside roseoside. Seven compounds were identified via UHPLC-UV method using reference compounds: rosmarinic acid, rutin, quercetin-3-O-glucoside, astragalin, apigenin-7-O-glucoside, apigenin-7-O-glucuronide and alcesefoliside, with the latter two being reported for the first time in genus Pulmonaria. The quantity of rosmarinic acid in PRE was 4.35%, distinguishing the compound as the main bioactive molecule in the species. Characterized by its high content of rosmarinic acid, P. rubra represents a highly viable candidate for subsequent development into standardized phytopharmaceuticals targeting oxidative stress-related neurodegenerative diseases. Full article
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