Extraction and Analysis of Edible Plant Natural Products: From Traditional to Innovative Technologies

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Plant Foods".

Deadline for manuscript submissions: 15 July 2026 | Viewed by 1123

Special Issue Editors


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Guest Editor
Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia
Interests: extraction; microreactors; mathematical modelling
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Guest Editor
Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
Interests: artificial neural network modeling; near-infrared spectroscopy; fermentation quality; NIR spectroscopy

Special Issue Information

Dear Colleagues,

The Special Issue, ‘Extraction and Analysis of Edible Plant Natural Products: From Traditional to Innovative Technologies’, aims to showcase recent progress, emerging trends and future perspectives in the study of bioactive compounds derived from edible plants. As global interest in natural products grows, driven by consumer demand for functional foods, nutraceuticals and sustainable ingredients, there is an urgent need to deepen scientific understanding of extraction processes, analytical methodologies and the bioefficacy of plant-based constituents.

This Special Issue will cover a comprehensive range of extraction strategies, spanning conventional methods as well as advanced green and sustainable techniques. These include ultrasound-assisted extraction, microwave-assisted extraction, supercritical fluid extraction, pressurized liquid extraction and enzyme-assisted processes. Emphasis will be placed on optimizing extraction efficiency, reducing environmental impact and improving selectivity for specific classes of phytochemicals such as polyphenols, flavonoids, alkaloids, carotenoids, terpenes and dietary fibers. Analytical approaches will form another central theme. Contributions may explore state-of-the-art qualitative and quantitative methods, including chromatography (HPLC, GC-MS, LC-MS/MS), spectroscopy (NMR, FTIR, UV-Vis), metabolomics, chemometrics and hyphenated techniques, for the identification, profiling and structural elucidation of plant natural products. Advances in data analysis, rapid screening tools and quality control frameworks suitable for food, pharmaceutical and cosmetic applications are also encouraged.

Furthermore, the Special Issue welcomes studies addressing the functional properties, bioavailability and health impacts of edible plant extracts, as well as research integrating extraction with downstream applications such as food fortification, encapsulation, formulation and product development. Interdisciplinary works that combine traditional knowledge with modern technological innovations are particularly valued. Overall, this Special Issue aims to provide a platform for researchers, industry experts and innovators to share knowledge that advances sustainable production, accurate characterization and beneficial utilization of edible plant natural products.

Dr. Ana Jurinjak Tušek
Dr. Tea Sokač Cvetnić
Guest Editors

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • edible plant natural products
  • green extraction technologies
  • phytochemical analysis
  • bioactive compounds
  • functional foods

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Published Papers (1 paper)

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Research

24 pages, 15878 KB  
Article
Phytochemical Enrichment of Carrot Seed Extracts by Ethanol-Modified Supercritical Fluid Extraction: Antimicrobial, Enzyme-Inhibitory, Butyrylcholinesterase Inhibition and Molecular Docking Investigations
by Husam Qanash, Sulaiman A. Alsalamah, Abdulrahman S. Bazaid, Fahad Almarshadi, Mohammed Ibrahim Alghonaim, Waleed Hakami, Amro Duhduh and Nourah M. Almimoni
Foods 2026, 15(10), 1721; https://doi.org/10.3390/foods15101721 - 13 May 2026
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Abstract
This study explored the impact of ethanol as a co-solvent in supercritical fluid extraction on the recovery of bioactive compounds from carrot seeds and assessed the resulting extracts for antimicrobial, α-amylase and α-glucosidase, and butyrylcholinesterase inhibitory potential. Ethanol supplementation significantly improved extraction performance, [...] Read more.
This study explored the impact of ethanol as a co-solvent in supercritical fluid extraction on the recovery of bioactive compounds from carrot seeds and assessed the resulting extracts for antimicrobial, α-amylase and α-glucosidase, and butyrylcholinesterase inhibitory potential. Ethanol supplementation significantly improved extraction performance, with the yield increasing from 110 mg in the absence of ethanol to 134 mg at 5% ethanol, followed by a slight decrease to 132 mg at 10%. High-performance liquid chromatography (HPLC) revealed pronounced phytochemical enrichment at 5% ethanol, particularly for chlorogenic acid (1541.24 µg/g), gallic acid (1279.27 µg/g), and hesperetin (1513.68 µg/g), indicating enhanced recovery of phenolic and flavonoid constituents. The 5% ethanol extract demonstrated superior antimicrobial activity, producing inhibition zones of 19 mm against Enterococcus faecalis, 26 mm against Klebsiella pneumoniae, 25 mm against Staphylococcus aureus, and 29 mm against Candida albicans. Values of both minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were markedly reduced, while antibiofilm activity reached 93.11% for E. faecalis and 91.00% for K. pneumoniae. The extract also exhibited potent inhibitory effects with IC50 values of 7.74 and 13.37 µg/mL, against α-amylase and α-glucosidase, correspondingly, as well as strong butyrylcholinesterase inhibition (IC50 = 2.51 µg/mL), highlighting promising α-amylase/α-glucosidase and butyrylcholinesterase inhibitory potential. Molecular docking further supported these findings, showing that chlorogenic acid bound more strongly than vanillin to OmpK36, lysosomal acid-α-glucosidase, and butyrylcholinesterase, with docking scores ranging from −6.1 to −6.9 kcal/mol. These findings identify ethanol-modified supercritical fluid extraction as a sustainable and effective green strategy for improving the recovery of carrot seed bioactives and enhancing their multifunctional in vitro biological properties. Notably, this study provides the first comprehensive evidence that 5% ethanol modification selectively enriches key phenolic constituents, including chlorogenic acid, gallic acid, and hesperetin, in carrot seed extracts, with corresponding enhancement of α-amylase, α-glucosidase, and butyrylcholinesterase inhibitory activities. Full article
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