Advances in Rainbow Trout: 2nd Edition

A special issue of Fishes (ISSN 2410-3888).

Deadline for manuscript submissions: closed (30 September 2025) | Viewed by 411

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Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China
Interests: aquaculture nutrition
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Dear Colleagues,

Rainbow trout (Oncorhynchus mykiss) holds a distinguished position in the realm of freshwater fisheries, aquaculture, and ecological research. As a species of paramount importance across the globe, their adaptability and economic value underline their significance in both natural habitats and controlled environments. This Special Issue aims to encapsulate the multifaceted dimensions of rainbow trout research, ranging from genetic enhancements, disease resistance, and nutritional advancements to sustainable aquaculture practices. Through a curated selection of cutting-edge research articles, we aspire to shed light on the latest scientific breakthroughs that are setting new frontiers in the study and cultivation of rainbow trout. Our objective is to provide a comprehensive overview that not only advances the current body of knowledge, but also inspires innovative approaches to address the ongoing challenges faced in rainbow trout research and aquaculture. We welcome contributions that explore novel methodologies, interdisciplinary studies, and reviews that forecast the future directions of rainbow trout studies. I look forward to your contributions.

Dr. Changan Wang
Guest Editor

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Keywords

  • rainbow trout
  • genetics
  • aquaculture technique
  • disease resistance
  • environmental adaptability
  • nutritional requirements
  • sustainable practices
  • ecological impact
  • genetic engineering
  • conservation strategies
  • aquatic ecosystems

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Published Papers (1 paper)

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Research

12 pages, 1952 KB  
Article
Development and Application of Infectious Hematopoietic Necrosis Virus Antigen-Specific DAS-ELISA Detection Method
by Jing-Zhuang Zhao, Min Wu, Li-Ming Xu, Yi-Zhi Shao, Wei-Tong Liu and Tong-Yan Lu
Fishes 2025, 10(10), 533; https://doi.org/10.3390/fishes10100533 - 20 Oct 2025
Viewed by 78
Abstract
Infectious hematopoietic necrosis virus (IHNV), a salmonid rhabdovirus, causes severe mortality exceeding 90% in both wild and farmed salmon and trout. Frequent outbreaks of IHNV highlight the urgent need for rapid detection methods to support effective prevention and control. This study developed a [...] Read more.
Infectious hematopoietic necrosis virus (IHNV), a salmonid rhabdovirus, causes severe mortality exceeding 90% in both wild and farmed salmon and trout. Frequent outbreaks of IHNV highlight the urgent need for rapid detection methods to support effective prevention and control. This study developed a double-antibody sandwich ELISA (DAS-ELISA) targeting the nucleocapsid (N) protein of IHNV. Two peptides derived from the N protein—selected for their strong antigenicity, high level of conservation, and surface accessibility—were used as immunogens to generate two specific monoclonal antibodies. Following optimization, the DAS-ELISA was established using monoclonal antibody N-15 as the capture antibody and horseradish peroxidase (HRP)-conjugated antibody N-106 as the detection antibody. The results of this study demonstrated that DAS-ELISA exhibited high specificity for multiple IHNV strains and showed no cross-reactivity with IPNV, SVCV, or VHSV. The detection sensitivity of DAS-ELISA for IHNV was determined to be 103 TCID50/mL. Parallel analysis of 293 clinical samples using DAS-ELISA and WOAH reference method demonstrated a concordance rate of 92.83% (κ = 0.856). These results confirm that the established DAS-ELISA exhibits high sensitivity, specificity, broad-spectrum applicability, and repeatability. In conclusion, this DAS-ELISA provides a reliable and efficient tool for high-throughput early detection of IHNV infection in clinical settings. Full article
(This article belongs to the Special Issue Advances in Rainbow Trout: 2nd Edition)
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