Advances in Rainbow Trout: 2nd Edition

A special issue of Fishes (ISSN 2410-3888).

Deadline for manuscript submissions: closed (30 September 2025) | Viewed by 1224

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Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, China
Interests: aquaculture nutrition
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Dear Colleagues,

Rainbow trout (Oncorhynchus mykiss) holds a distinguished position in the realm of freshwater fisheries, aquaculture, and ecological research. As a species of paramount importance across the globe, their adaptability and economic value underline their significance in both natural habitats and controlled environments. This Special Issue aims to encapsulate the multifaceted dimensions of rainbow trout research, ranging from genetic enhancements, disease resistance, and nutritional advancements to sustainable aquaculture practices. Through a curated selection of cutting-edge research articles, we aspire to shed light on the latest scientific breakthroughs that are setting new frontiers in the study and cultivation of rainbow trout. Our objective is to provide a comprehensive overview that not only advances the current body of knowledge, but also inspires innovative approaches to address the ongoing challenges faced in rainbow trout research and aquaculture. We welcome contributions that explore novel methodologies, interdisciplinary studies, and reviews that forecast the future directions of rainbow trout studies. I look forward to your contributions.

Dr. Changan Wang
Guest Editor

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Keywords

  • rainbow trout
  • genetics
  • aquaculture technique
  • disease resistance
  • environmental adaptability
  • nutritional requirements
  • sustainable practices
  • ecological impact
  • genetic engineering
  • conservation strategies
  • aquatic ecosystems

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Related Special Issue

Published Papers (2 papers)

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Research

24 pages, 3626 KB  
Article
Dietary Clostridium butyricum and Alanyl-Glutamine Modulate Low-Fishmeal-Induced Growth Reduction, Intestinal Microbiota Disorders, Intestinal Inflammatory Injury, and Resistance Against Aeromonas salmonicida in Triploid Oncorhynchus mykiss
by Siyuan Liu, Li Chen, Shuze Zhang, Yaling Wang, Shaoxia Lu, Shicheng Han, Haibo Jiang, Hongbai Liu and Chang’an Wang
Fishes 2025, 10(11), 555; https://doi.org/10.3390/fishes10110555 - 2 Nov 2025
Viewed by 393
Abstract
Low-fishmeal feed is increasingly being adopted across the global aquaculture industry. This study evaluated dietary Clostridium butyricum and alanyl-glutamine (Ala-Gln) supplementation in juvenile triploid rainbow trout (Oncorhynchus mykiss) with a low-fishmeal diet. Four diets were tested: basal diet (SBM, 15% fishmeal [...] Read more.
Low-fishmeal feed is increasingly being adopted across the global aquaculture industry. This study evaluated dietary Clostridium butyricum and alanyl-glutamine (Ala-Gln) supplementation in juvenile triploid rainbow trout (Oncorhynchus mykiss) with a low-fishmeal diet. Four diets were tested: basal diet (SBM, 15% fishmeal and 21.6% soybean meal), SBM + 0.5% C. butyricum (CB), SBM + 1.0% Ala-Gln, and SBM + 0.5% C. butyricum + 1.0% Ala-Gln (CB-AG). Fish were fed in 500 L tanks in recirculating aquaculture systems for 8 weeks (62.52 ± 0.47 g). Each group comprised three tanks, with each tank housing 30 fish. Then 10 fish per tank were challenged with Aeromonas salmonicida. CB-AG showed significantly higher weight gain and specific growth rates than the SBM group (p < 0.05). Mortality was significantly lower in CB-AG and AG than in SBM after A. salmonicida challenge. Histomorphology revealed significant differences (p < 0.05) between CB-AG and SBM in muscularis thickness, villus width, and height. SBM sections showed inflammatory infiltration and border damage were attenuated in supplemented groups. Serum malondialdehyde (MDA) and dioxygenase (DAO) were significantly lower in CB-AG than SBM (p < 0.05), while serum and hepatic lysozyme (LZM) and hepatic superoxide dismutase (SOD) were higher. Digestive enzymes indicated significantly higher trypsin and lipase activities in CB-AG (p < 0.05). CB-AG upregulated intestinal tight junction proteins and PepT1 and downregulated pro-inflammatory mediators. Combined 0.5% C. butyricum and 1.0% Ala-Gln inclusion effectively preserved growth performance, antioxidant capacity, gut microbiome homeostasis, and intestinal health in rainbow trout on low-fishmeal diets. Full article
(This article belongs to the Special Issue Advances in Rainbow Trout: 2nd Edition)
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12 pages, 1952 KB  
Article
Development and Application of Infectious Hematopoietic Necrosis Virus Antigen-Specific DAS-ELISA Detection Method
by Jing-Zhuang Zhao, Min Wu, Li-Ming Xu, Yi-Zhi Shao, Wei-Tong Liu and Tong-Yan Lu
Fishes 2025, 10(10), 533; https://doi.org/10.3390/fishes10100533 - 20 Oct 2025
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Abstract
Infectious hematopoietic necrosis virus (IHNV), a salmonid rhabdovirus, causes severe mortality exceeding 90% in both wild and farmed salmon and trout. Frequent outbreaks of IHNV highlight the urgent need for rapid detection methods to support effective prevention and control. This study developed a [...] Read more.
Infectious hematopoietic necrosis virus (IHNV), a salmonid rhabdovirus, causes severe mortality exceeding 90% in both wild and farmed salmon and trout. Frequent outbreaks of IHNV highlight the urgent need for rapid detection methods to support effective prevention and control. This study developed a double-antibody sandwich ELISA (DAS-ELISA) targeting the nucleocapsid (N) protein of IHNV. Two peptides derived from the N protein—selected for their strong antigenicity, high level of conservation, and surface accessibility—were used as immunogens to generate two specific monoclonal antibodies. Following optimization, the DAS-ELISA was established using monoclonal antibody N-15 as the capture antibody and horseradish peroxidase (HRP)-conjugated antibody N-106 as the detection antibody. The results of this study demonstrated that DAS-ELISA exhibited high specificity for multiple IHNV strains and showed no cross-reactivity with IPNV, SVCV, or VHSV. The detection sensitivity of DAS-ELISA for IHNV was determined to be 103 TCID50/mL. Parallel analysis of 293 clinical samples using DAS-ELISA and WOAH reference method demonstrated a concordance rate of 92.83% (κ = 0.856). These results confirm that the established DAS-ELISA exhibits high sensitivity, specificity, broad-spectrum applicability, and repeatability. In conclusion, this DAS-ELISA provides a reliable and efficient tool for high-throughput early detection of IHNV infection in clinical settings. Full article
(This article belongs to the Special Issue Advances in Rainbow Trout: 2nd Edition)
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