Dairy Fermentation from a Microbial Perspective

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Fermentation for Food and Beverages".

Deadline for manuscript submissions: 31 March 2027 | Viewed by 123

Editors


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Guest Editor
Department of Food Engineering, Faculty of Agriculture, Atatürk University, 25240 Erzurum, Turkey
Interests: food engineering; engineering and technology

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Guest Editor Assistant
Department of Food Engineering, Faculty of Agriculture, Ataturk University, Erzurum, Turkey
Interests: food microbiology; food toxicology; food biotechnology

Special Issue Information

Dear Colleagues,

Dairy fermentation represents one of the oldest and most sophisticated biotechnological processes, driven by complex and dynamic microbial communities. Microorganisms, primarily lactic acid bacteria, but also yeasts and molds, play pivotal roles in determining the safety, quality, texture, flavor, and nutritional value of fermented dairy products. With the advancement of molecular and omics based techniques, our understanding of microbial diversity, metabolic pathways, and interspecies interactions in dairy systems has significantly deepened.

We are pleased to invite you to contribute to this Special Issue entitled “Dairy Fermentation from a Microbial Perspective”. This issue will focus on recent scientific advances that elucidate the roles, functionalities, and applications of microorganisms in dairy fermentation processes, from traditional artisanal products to modern industrial systems.

This Special Issue aims to gather high quality original research articles and comprehensive reviews that explore microbial driven transformations in dairy matrices. The topic aligns closely with the journal’s scope by integrating food microbiology, fermentation technology, and functional food development. Emphasis will be placed on studies that provide mechanistic insights, innovative methodologies, and translational applications in dairy fermentation.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following:

  • Dairy-related in vitro fermentation models and gastrointestinal simulation systems;
  • Microbial diversity and community dynamics in fermented dairy products;
  • Isolation, identification, and functional characterization of dairy associated microorganisms;
  • Starter and adjunct cultures: selection, optimization, and industrial performance;
  • Microbial interactions in dairy fermentations (bacteria, yeast, mold consortia);
  • Metabolic pathways and bioactive compound production in dairy systems;
  • Application of omics technologies (genomics, transcriptomics, proteomics, metabolomics) in dairy microbiology;
  • Probiotic microorganisms in dairy products: functionality, viability, and health implications;
  • Microbial contributions to flavor, texture, and quality development;
  • Safety aspects, including antimicrobial resistance, biogenic amines, and risk assessment;
  • Innovations in dairy fermentation processes and biotechnological applications;
  • Microbial adaptation and stress responses in dairy environments;
  • Comparative studies of traditional vs. industrial dairy fermentations.

We look forward to receiving your contributions.

Dr. Tuba Erkaya
Guest Editor

Dr. Hacer Meral Aktaş
Guest Editor Assistant

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • dairy fermentation
  • dairy microbiology
  • lactic acid bacteria
  • starter cultures
  • probiotics
  • microbiome
  • metabolomics
  • food safety
  • bioprocessing
  • microbial interaction

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Published Papers (1 paper)

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Research

18 pages, 2503 KB  
Article
Effect of Pre- and Post-Fermentation Coffee Addition on the Physicochemical, Microbiological and Sensory Properties of Kefir During Refrigerated Storage
by Cennet Acar, Deniz Koçan and Fatma Şahmurat
Fermentation 2026, 12(8), 346; https://doi.org/10.3390/fermentation12080346 - 27 Jul 2026
Abstract
Kefir is a self-carbonated fermented milk produced with kefir grains which is valued for its complex symbiotic microbiota and probiotic potential. Flavouring kefir is a recognised strategy to broaden consumer appeal, yet the moment at which a flavouring ingredient is introduced—before or after [...] Read more.
Kefir is a self-carbonated fermented milk produced with kefir grains which is valued for its complex symbiotic microbiota and probiotic potential. Flavouring kefir is a recognised strategy to broaden consumer appeal, yet the moment at which a flavouring ingredient is introduced—before or after fermentation—may differentially affect the product. The present study evaluated the effect of the timing of instant coffee addition on the physicochemical, microbiological and sensory properties of kefir during 14 days of refrigerated storage (4 ± 1 °C). Three kefir samples were produced from UHT cow’s milk (3% fat) inoculated with 3% (w/v) kefir grains: a control without coffee (K), kefir with 0.5% (w/v) instant coffee added before fermentation (PreF_CK) and kefir with 0.5% (w/v) instant coffee added after fermentation and maturation (PostF_CK). Samples were analysed on days 1, 7 and 14. The coffee addition significantly increased the total dry matter (K = 10.72%; PreF_CK = 11.04%; PostF_CK = 11.00%; p < 0.01) and slightly modified the titratable acidity and pH (p < 0.05), whereas the ash content (expressed in terms of dry matter) did not differ among samples (p > 0.05). The titratable acidity increased and the pH decreased with storage in all samples (p < 0.05). Crucially, the coffee addition—irrespective of the timing—did not impair the viability of the kefir microbiota; the total mesophilic aerobic bacteria, Lactobacillus spp., mesophilic lactic cocci and yeast counts did not differ significantly among the samples (p > 0.05) and remained high throughout storage (lactic acid bacteria ≥8 log CFU/g; yeast ≥5.7 log CFU/g), exceeding the minimum thresholds of the standards for fermented milk products. Sensory evaluation showed that the control achieved the highest odour and taste scores (p < 0.001), while among the coffee-containing samples, the post-fermentation addition (PostF_CK) was generally scored at least as high as the pre-fermentation addition (PreF_CK); overall acceptability did not differ significantly among the samples (p > 0.05). These findings demonstrate that 0.5% instant coffee can be incorporated into kefir without compromising its characteristic microbiota or storage stability and that post-fermentation addition is a viable route for producing an acceptable coffee-flavoured kefir. Full article
(This article belongs to the Special Issue Dairy Fermentation from a Microbial Perspective)
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