Progress in Microbial Treatment of Wastewater, Solid Wastes and Waste Gases, 2nd Edition

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Industrial Fermentation".

Deadline for manuscript submissions: 31 August 2025 | Viewed by 5677

Special Issue Editors


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Guest Editor
College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China
Interests: microbiome; synthetic biology; microbial CO2 conversion; biofuel
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
College of Environmental Science and Engineering, Beijing Forestry University, Beijing 100083, China
Interests: anaerobic digestion; methanogenesis; carbon dioxide bioreduction; waste biotreatment; microbial electrolysis system
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Key Laboratory for Heavy Metal Pollution Control and Reutilization, School of Environment and Energy, Peking University Shenzhen Graduate School, Shenzhen 518055, China
Interests: meta-omics; aerobic denitrification; carbon fixation; heavy metals
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microbes are key players in biogeochemical cycles due to their functions of macromolecule degradation, compound conversion, and element enrichment. Therefore, microbe-based technologies have been developed rapidly to treat wastewater (such as anammox, bioelectrochemical systems, and algae–bacteria systems), solid wastes (such as aerobic composting, anaerobic digestion, and biofuel production), and waste gases (such as syngas fermentation and microbial electrosynthesis). This Special Issue aims to provide a platform for global researchers to disseminate recent technological developments and engineering solutions in microbial treatment of wastewater, solid wastes, and waste gases. Original research articles, critical reviews, and perspectives are welcome to be submitted to this Special Issue, where potential topics include but are not limited to the following:

  • The exploitation of microbes as tools for treating wastewater, solid wastes, and waste gases.
  • Mechanism analysis of microbial treatment of wastewater, solid wastes, and waste gases, e.g., community structures and functional compositions, activities, and dynamics of microbes in the treatment systems.
  • New technologies or processes for microbial treatment of wastewater, solid wastes, and waste gases.
  • Performance improvement of microbial treatment of wastewater, solid wastes, and waste gases based on synthetic biology, new materials, etc.
  • Modeling and economic analyses for microbial processes of wastewater treatment, solid waste treatment, and waste gas conversion.

Dr. Pengsong Li
Prof. Dr. Yan Dang
Dr. Lijuan Zhang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fermentation is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • aerobic composting
  • algal–bacterial consortium
  • anaerobic digestion
  • bioelectrochemical system
  • biofuel
  • meta-omics
  • microbial electrosynthesis
  • microbial fuel cell
  • microbial wastewater treatment
  • syngas fermentation

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Related Special Issue

Published Papers (4 papers)

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Research

24 pages, 2432 KiB  
Article
Biohydrogen and Biobutanol Production from Spent Coffee and Tea Waste Using Clostridium beijerinckii
by Stephen Abiola Akinola, Beenish Saba, Ann Christy, Katrina Cornish and Thaddeus Chukwuemeka Ezeji
Fermentation 2025, 11(4), 177; https://doi.org/10.3390/fermentation11040177 - 28 Mar 2025
Viewed by 390
Abstract
The growing advocacy for greener climates, coupled with increasing global energy demand driven by urbanization and population growth, highlights the need for sustainable solutions. Repurposing food wastes as substrates offers a promising approach to enhancing cleaner energy generation and promoting a circular economy. [...] Read more.
The growing advocacy for greener climates, coupled with increasing global energy demand driven by urbanization and population growth, highlights the need for sustainable solutions. Repurposing food wastes as substrates offers a promising approach to enhancing cleaner energy generation and promoting a circular economy. This study investigated the potential of spent coffee grounds (SC) and biosolids cake (BS) from tea wastes as substrates for producing valuable fuels and chemicals through acetone–ethanol–butanol (ABE) fermentation. Clostridium beijerinckii NCIMB 8052 was used to ferment 100% and 50% hydrolysates derived from Parr-treated enzyme-hydrolyzed (PEH, PEH50), Parr-treated non-hydrolyzed (PNEH, PNEH50), and non-Parr-treated hydrolyzed (NPEH) SC wastes, as well as enzyme-hydrolyzed (BSH, BSH50) and non-hydrolyzed BS wastes (NBH, NBH50). Fermentation of unmodified hydrolysates by C. beijerinckii was poor. Following CaCO3 modification of SC and BS hydrolysates, ABE titer, yield, and productivity increased, with the highest values obtained with PEH50 and NBH. Specifically, CaCO3 modification of SC hydrolysates led to increased butanol titer, yield, and productivity in PEH50, while the NBH exhibited higher butanol yield and productivity than the non-CaCO3-modified hydrolysates. Additionally, H2 gas production with PEH50 and NBH was 1.41- and 1.13-fold higher, respectively, than in other hydrolysates. These findings suggest that SC and BS hydrolysates can be valorized to butanol and hydrogen gas and, thereby, can contribute to global food wastes management, energy sustainability, and cost-effective biofuel production. Full article
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13 pages, 6956 KiB  
Article
Clostridia as Promising Biofactory for Heterotrophic (Fructose) and Autotrophic (C1-Gas) Fermentation
by Marina Fernández-Delgado, Mónica Coca, Susana Lucas, María Teresa García-Cubero and Juan Carlos López-Linares
Fermentation 2024, 10(11), 572; https://doi.org/10.3390/fermentation10110572 - 8 Nov 2024
Cited by 1 | Viewed by 1266
Abstract
This study compared the performance of Clostridium ljungdahlii and Clostridium aceticum in the fermentation of fructose and C1-gasses (CO, CO2, N2) to produce valuable products such as ethanol and acetic acid. In heterotrophic fermentation (fructose), C. ljungdahlii yielded high [...] Read more.
This study compared the performance of Clostridium ljungdahlii and Clostridium aceticum in the fermentation of fructose and C1-gasses (CO, CO2, N2) to produce valuable products such as ethanol and acetic acid. In heterotrophic fermentation (fructose), C. ljungdahlii yielded high ethanol concentrations (350 mg/L) and acetic acid (500 mg/L), with optimal production at pH 8 on the first day of fermentation. Although autotrophic fermentation (C1-gasses) resulted in lower ethanol levels (200 mg/L), it remained a viable option. Conversely, C. aceticum predominantly produced acetic acid in both fermentation modes, with higher concentrations in the heterotrophic fermentation (1600 mg/L) than the autotrophic fermentation (380 mg/L). These findings demonstrate the versatility of both microorganisms for producing valuable metabolites. C. ljungdahlii shows promise for bioethanol production, while C. aceticum excels at generating acetic acid, a crucial component in bioplastics and various industrial processes. Full article
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12 pages, 2548 KiB  
Article
In Situ Enrichment of Anammox Bacteria from Pig Farm Anoxic Sludge Through Co-Cultivation with a Quorum-Sensing Functional Strain Pseudomonas aeruginosa
by Yong Liu, Yiru Zhu, Jianping Deng, Bing Yan, Jian Zhan, Yuansong Wei, Hanbing Nie and Shuanglin Gui
Fermentation 2024, 10(11), 548; https://doi.org/10.3390/fermentation10110548 - 25 Oct 2024
Viewed by 1003
Abstract
Anaerobic ammonium oxidation (anammox), as an efficient and low-carbon method for nitrogen removal from wastewater, faces the challenge of slow enrichment of functional bacteria. In this study, the enrichment of anammox bacteria Candidatus Brocadia was successfully accelerated by co-culturing with the quorum-sensing strain [...] Read more.
Anaerobic ammonium oxidation (anammox), as an efficient and low-carbon method for nitrogen removal from wastewater, faces the challenge of slow enrichment of functional bacteria. In this study, the enrichment of anammox bacteria Candidatus Brocadia was successfully accelerated by co-culturing with the quorum-sensing strain Pseudomonas aeruginosa and anoxic sludge from a pig farm. Experimental results showed that the R2, which had Pseudomonas aeruginosa added, exhibited chemical reaction ratios RS (NO2-N consumption/NH4+-N consumption) and RP (NO3-N production/NH4+-N consumption) closer to the theoretical values of the anammox reaction since Phase Ⅱ. Bacterial community analysis indicated that the abundance of Candidatus Brocadia in R2 reached 1.63% in cycle 20, significantly higher than the 0.45% in R1. More quorum-sensing signaling molecules, primarily C6-HSL, were detected in R2. C6-HSL was positively correlated with processes such as the secretion of anammox extracellular polymers (EPS) and the regulation of nitric oxide reductase (Nir), which may explain the reason behind the accelerated increase in the abundance of Candidatus Brocadia through co-culturing. Moreover, the metabolism of the dominant genus Paracoccus within the two groups of reactors also showed positive regulation by C6-HSL, with its abundance trend similar to that of Candidatus Brocadia, jointly completing the nitrogen removal process in the reactors. However, it is still unknown which genera secrete large amounts of C6-HSL after inoculation with Pseudomonas aeruginosa. This research provides a novel and low-cost method for the enrichment of anammox bacteria. Full article
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21 pages, 11026 KiB  
Article
Combined Use of Biochar and Microbial Agents Can Promote Lignocellulosic Degradation Microbial Community Optimization during Composting of Submerged Plants
by Hongjie Wang, Zhiwei Su, Shengnan Ren, Panyue Zhang, Hui Li, Xiaoping Guo and Ling Liu
Fermentation 2024, 10(1), 70; https://doi.org/10.3390/fermentation10010070 - 21 Jan 2024
Cited by 4 | Viewed by 2262
Abstract
Aerobic composting is one of the methods for the resource utilization of submerged plant residues. This study investigated the effects of biochar, wetland sediments and microbial agents added individually or combined on the humification process, lignocellulose degradation and microbial communities during Ceratophyllum demersum [...] Read more.
Aerobic composting is one of the methods for the resource utilization of submerged plant residues. This study investigated the effects of biochar, wetland sediments and microbial agents added individually or combined on the humification process, lignocellulose degradation and microbial communities during Ceratophyllum demersum and Potamogeton wrightii composting. The results showed that the addition of wetland sediment and biochar was found to significantly elevate the composting temperature and humification of compost products. The average content of lignin in wetland sediment and/or biochar treatments was 12.2–13.5%, which was higher than the control group (10.9–11.45%). Compared with the organic matter (19.4%) and total nitrogen concentration (35.3%) of compost treated with complex microbial agent treatments, the homemade microbial agents significantly increased the values by 22.1% and 41.0%, respectively. By comparing the differences in microbial communities among different treatments, the sediments and homemade agents demonstrated greater increases in activity and diversity of lignocellulose degradation-related microbes, especially for Truepera and Actinomarinale. Humus component and temperature were the most critical parameters influencing the changes in the bacterial community. Based on these results, a combination of biochar and homemade agents was a promising additive for an effective composting strategy, and sediment was identified as a potential control of bacterial diversity in wetland plant compost. Full article
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