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Diagnostics
Special Issues
Biofilm Bacteria Diagnostics in Managing Chronic Infections
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Biofilm Bacteria Diagnostics in Managing Chronic Infections

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Point-of-Care Diagnostics and Devices".

Deadline for manuscript submissions: closed (31 January 2022) | Viewed by 2305

Special Issue Editor

Dr. James Bowen Stiehl

E-Mail Website
Guest Editor
Department of Surgery, St. Mary's Hospital, 1054 Martin Luther King Drive, Centralia, IL 62801, USA
Interests: chronic infections; biomechanics; computer; robotic technologies

Special Issue Information

Dear Colleagues,

This symposium will explore technologies across medicine that function to understand, diagnose, and guide treatments for biofilm bacterial chronic infections. As biofilm bacteria are found to be ubiquitous in nature and medicine, it is anticipated that this will be a collaborative, multidiscipline review.  Important technologies of this generation include polymerase chain reaction sequencing, confocal laser microscopy, and autofluorescence imaging to name just a few. The enlightened medical clinician must have a working understanding of how these technologies are applied, and then they must gain confidence in the implications that they create for medical treatment.  In-depth analysis of how a technology works along with metrology and statistical methods to define measurements will frame the implications and reliability for the practicing clinician. Great physicians want to know the how, what, and why and practice outside of the box or ‘cookbook’.

Dr. James Stiehl
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Biofilm
  • Molecular diagnostics
  • Autofluorescence imaging
  • Chronic imaging

Published Papers (1 paper)

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Research

16 pages, 3717 KiB  
Article
New Adapted In Vitro Technology to Evaluate Biofilm Formation and Antibiotic Activity Using Live Imaging under Flow Conditions
by Cassandra Pouget, Catherine Dunyach-Remy, Alix Pantel, Sophie Schuldiner, Albert Sotto and Jean-Philippe Lavigne
Diagnostics 2021, 11(10), 1746; https://doi.org/10.3390/diagnostics11101746 - 23 Sep 2021
Cited by 9 | Viewed by 1889
Abstract
The polymicrobial nature of biofilms and bacterial interactions inside chronic wounds are keys for the understanding of bacterial cooperation. The aim of this present study was to develop a technique to study and visualize biofilm in live imaging under flow conditions (Bioflux™ 200, [...] Read more.
The polymicrobial nature of biofilms and bacterial interactions inside chronic wounds are keys for the understanding of bacterial cooperation. The aim of this present study was to develop a technique to study and visualize biofilm in live imaging under flow conditions (Bioflux™ 200, Fluxion Biosciences). The BiofluxTM system was adapted using an in vitro chronic wound-like medium (CWM) that mimics the environment encountered in ulcers. Two reference strains of Staphylococcus aureus (Newman) and Pseudomonas aeruginosa (PAO1) were injected in the BiofluxTM during 24 h to 72 h in mono and coculture (ratio 1:1, bacteria added simultaneously) in the CWM vs. a control medium (BHI). The quantification of biofilm formation at each time was evaluated by inverted microscopy. After 72 h, different antibiotics (ceftazidime, imipenem, linezolid, oxacillin and vancomycin) at 1x MIC, 10x MIC and 100x MIC were administrated to the system after an automatic increase of the flow that mimicked a debridement of the wound surface. Biofilm studies highlighted that the two species, alone or associated, constituted a faster and thicker biofilm in the CWM compared to the BHI medium. The effect of antibiotics on mature or “debrided” biofilm indicated that some of the most clinically used antibiotic such as vancomycin or imipenem were not able to disrupt and reduce the biofilm biomass. The use of a life cell imaging with an in vitro CWM represents a promising tool to study bacterial biofilm and investigate microbial cooperation in a chronic wound context. Full article
(This article belongs to the Special Issue Biofilm Bacteria Diagnostics in Managing Chronic Infections)
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