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Diagnostics
Special Issues
Microbiology Laboratory: Sample Collection and Diagnosis Advances, 2nd Edition
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Microbiology Laboratory: Sample Collection and Diagnosis Advances, 2nd Edition

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Diagnostic Microbiology and Infectious Disease".

Deadline for manuscript submissions: 30 June 2025 | Viewed by 598

Special Issue Editors

Prof. Dr. James P. Chambers

E-Mail Website
Guest Editor
Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA
Interests: biochemistry; pathogen detection; virology; bacteriology; diagnostics; biosensors
Special Issues, Collections and Topics in MDPI journals
Dr. Luke T. Daum

E-Mail
Guest Editor
LuJo BioScience Laboratory, 1747 Citadel Plaza, Suite 201, San Antonio, TX 78209, USA
Interests: sample collection; nucleic acid extraction; qPCR; NGS; sequencing; molecular epidemiology; tuberculosis; influenza; SARS-CoV-2; diagnostics; point of care; rapid detection
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Infectious microbes continue to emerge, causing persistent infections, re-infections, and epidemics worldwide. Advances in the detection of nucleic acids, antigens, and protein assays, as well as the genomic characterization of drug susceptibility markers, have improved microbial diagnosis and treatment. Furthermore, innovations in sample collection, pre-processing, home collection, and point-of-care/decentralized testing have expanded the reach of microbial diagnosis to new patients, populations, and resource-challenged environments. We welcome the submission of manuscripts related to novel advances in the microbial diagnosis of infectious diseases, including but not limited to the fields of virology, bacteriology, mycology, and molecular epidemiology. Acceptable submission types include research articles, short research notes, and reviews.

Prof. Dr. James P. Chambers
Dr. Luke T. Daum
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • qPCR
  • NGS
  • sample collection
  • nucleic acid testing
  • rapid antigen testing
  • infectious diseases
  • virology
  • sequencing
  • point-of-care
  • diagnostics
  • bacteriology diagnosis
  • biosensors

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Related Special Issue

Published Papers (1 paper)

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Research

8 pages, 636 KiB  
Communication
Integrating an LFA Carbapenemase Detection System into the Laboratory Diagnostic Routine: Preliminary Data and Effectiveness Against Enzyme Variants
by Maddalena Calvo, Gaetano Maugeri, Dafne Bongiorno, Giuseppe Migliorisi and Stefania Stefani
Diagnostics 2025, 15(11), 1434; https://doi.org/10.3390/diagnostics15111434 - 5 Jun 2025
Viewed by 349
Abstract
Background/Objectives. Carbapenemase production is the most diffused carbapenem-resistance mechanism among Enterobacterales, with Klebsiella pneumoniae carbapenemase (KPC), Verona-imipenemase (VIM), New-Delhi metallo-β-lactamase (NDM), imipenemase (IMP), and oxacillinase (OXA-48) being reported as the main types within Europe. Particularly, Southern Italy holds a concerningly high [...] Read more.
Background/Objectives. Carbapenemase production is the most diffused carbapenem-resistance mechanism among Enterobacterales, with Klebsiella pneumoniae carbapenemase (KPC), Verona-imipenemase (VIM), New-Delhi metallo-β-lactamase (NDM), imipenemase (IMP), and oxacillinase (OXA-48) being reported as the main types within Europe. Particularly, Southern Italy holds a concerningly high percentage of carbapenemases-producing Enterobacterales diffused among different hospital settings. These strains may colonize critical patients’ gastrointestinal tracts, often causing disseminations and severe complications. Scientific data recently reported carbapenemase variants’ worldwide diffusion and several double-carbapenemases reports. The diagnostic routine needs devices whose detection rates are extended to similar epidemiological conditions, avoiding a lack of specificity and potential negative results. Methods. We planned a retrospective study including carbapenem- and/or ceftazidime/avibactam-resistant Enterobacterales (62) which were tested with the KPC/IMP/NDM/VIM/OXA-48 Combo Test Kit (KINVO, Medomics Medical Technology, Nanjing, Jiangsu, China) based on the lateral flow assay (LFA) method. Results. We compared its results to the phenotypic antimicrobial susceptibility testing (AST) MIC results, obtaining a 100% agreement rate. The LFA kit reported carbapenemases in all the tested strains, also identifying cases of KPC variants and double-carbapenemases production. Conclusions. Our data demonstrated how LFAs may represent a reliable alternative requiring minimum economic and personnel resources along with simple result interpretations. Future studies will be necessary to further investigate the system effectiveness on a larger isolates’ number and a broad carbapenemase variant spectrum. Full article
(This article belongs to the Special Issue Microbiology Laboratory: Sample Collection and Diagnosis Advances, 2nd Edition)
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