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Chemosensors 2018, 6(3), 40; https://doi.org/10.3390/chemosensors6030040

“Probe, Sample, and Instrument (PSI)”: The Hat-Trick for Fluorescence Live Cell Imaging

1
Normandie Université, UNIROUEN, INSERM, PRIMACEN, 76000 Rouen, France
2
Normandie Université, CNRS, INSA Rouen, UNIROUEN, COBRA, 76000 Rouen, France
3
Normandie Université, UNIROUEN, Laboratoire Glycobiologie et Matrice Extracellulaire végétale (Glyco-MEV) EA4358, 76821 Mont-Saint-Aignan, France
4
Department of Neuroscience, School of Medicine, Yale University, New Haven, CT 06510, USA
*
Author to whom correspondence should be addressed.
Received: 14 June 2018 / Revised: 23 August 2018 / Accepted: 10 September 2018 / Published: 13 September 2018
(This article belongs to the Special Issue Fluorescent Probes for Live Cell Imaging)
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Abstract

Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice of adapted fluorescent probes for live cell imaging. What to detect in what type of sample and for how long is a major issue with fluorescent probes and, for this, the “hat-trick” “Probe–Sample–Instrument” (PSI) has to be considered. We propose here to deal with key points usually discussed in CIPs including the properties of fluorescent organic probes, the modality of cell labeling, and the best equipment to obtain appropriate spectral, spatial, and temporal resolution. New strategies in organic synthesis and click chemistry for accessing probes with enhanced photophysical characteristics and targeting abilities will also be addressed. Finally, methods for image processing will be described to optimize exploitation of fluorescence signals. View Full-Text
Keywords: fluorescent probes; small fluorescent organic molecules; fluorescent proteins; cell labeling; live cell imaging; photophysical properties; organic synthesis; click chemistry; advanced microscopy; instrumental performances; image processing fluorescent probes; small fluorescent organic molecules; fluorescent proteins; cell labeling; live cell imaging; photophysical properties; organic synthesis; click chemistry; advanced microscopy; instrumental performances; image processing
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Galas, L.; Gallavardin, T.; Bénard, M.; Lehner, A.; Schapman, D.; Lebon, A.; Komuro, H.; Lerouge, P.; Leleu, S.; Franck, X. “Probe, Sample, and Instrument (PSI)”: The Hat-Trick for Fluorescence Live Cell Imaging. Chemosensors 2018, 6, 40.

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