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Cells
Special Issues
Novel Insights into the Biofilms
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Novel Insights into the Biofilms

A special issue of Cells (ISSN 2073-4409).

Deadline for manuscript submissions: closed (20 March 2025) | Viewed by 3029

Special Issue Editors

Prof. Dr. Sylvie Chevalier

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Guest Editor
Laboratoire de Microbiologie Signaux et Microenvironnement, LMSM EA4312, Université de Rouen Normandie, IUT Evreux - 55 rue St Germain, 27000 Evreux, France
Interests: bacterial stress response; adaptation; biofilm and virulence; antimicrobial strategies
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Pierre Cornelis

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Guest Editor
Laboratoire de Microbiologie Communication Bactérienne et Stratégies Anti-infectieuses, CBSA UR4312, Université de Rouen Normandie, IUT Evreux - 55 rue St Germain, 27000 Evreux, France
Interests: bacterial biofilm; pathogenicity; antimicrobial strategies

Special Issue Information

Dear Colleagues,

It is well known that bacteria can switch from a planktonic unicelluar multiplication to a sessile non-motile assembly of cells encased in a extracellular matrix composed of polysaccharides, DNA, and proteins. Such multicellular structure forms what is called a biofilm. Biofilms are either attached to a surface such as epithelial cells or present as 3-dimensional aggregates as observed in the lungs of cystic fibrosis patients infected by Pseudomonas aeruginosa, a model microorganism for the study of biofilms. Bacterial biofilms are a dynamic structure evolving from attachment, microcolonies formation, maturation and decay allowing dispersion of newly formed planktonic cells. These different steps involve a cascade of several regulatory processes. Much attention focuses on ways to inhibit biofilm formation or to disperse already formed bofilms since planktonic cells are more sensitive to antibiotic treatments compared to the sessile cells protected by the extracellular matrix. This special issue on biofilms is open to all scientists involved in bacterial biofilm research including those interested in the regulation of biofilm development, biofilm dispersion, antibiotic and drug resistance, cells differentiation, matrix composition, mixed biofilms, and other aspects of biofilm research. Contributions focusing on the model microorganisms Pseudomonas aeruginosa and Staphylococcus aureus as well as studies on other bacterial species  are welcome.

Prof. Dr. Sylvie Chevalier
Prof. Dr. Pierre Cornelis
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cells is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biofilms maturation
  • biofilm matrix
  • regulation
  • dispersion
  • eradication

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Published Papers (2 papers)

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Research

17 pages, 2387 KiB  
Article
Antimicrobial Blue Light Reduces Human-Wound Pathogens’ Resistance to Tetracycline-Class Antibiotics in Biofilms
by Laisa Bonafim Negri, Sandeep Korupolu, William Farinelli, Alexis K. Jolly, Robert W. Redmond, Shifu Aggarwal, Laurence G. Rahme, Kristin H. Gilchrist, R. Rox Anderson and Jeffrey A. Gelfand
Cells 2025, 14(3), 219; https://doi.org/10.3390/cells14030219 - 4 Feb 2025
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Abstract
Biofilms contribute to chronic infections and the development of antimicrobial resistance (AMR). We are developing an antimicrobial blue light (aBL) device to reduce bacterial bioburden in wounds and decrease reliance on systemic antibiotics. aBL induces the generation of reactive oxygen species (ROS) through [...] Read more.
Biofilms contribute to chronic infections and the development of antimicrobial resistance (AMR). We are developing an antimicrobial blue light (aBL) device to reduce bacterial bioburden in wounds and decrease reliance on systemic antibiotics. aBL induces the generation of reactive oxygen species (ROS) through photoexcitation of endogenous chromophores, causing bacterial damage and death. This study explores the combination of tetracyclines (TCs) with aBL for the treatment of biofilm infections in vitro. Tetracyclines (TCs), including second-generation minocycline (MC), doxycycline (DOCT), and third-generation agents omadacycline (OM) and tigecycline (TG), were evaluated for their ability to enhance bactericidal effects and ROS production during aBL treatment of abiotic biofilm. TCs were tested under dark conditions and with varying aBL light parameters against biofilms of methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa (PA), and Escherichia coli (E. coli). Results showed that TCs alone were ineffective against these biofilm cultures. However, when combined with aBL either before or after TC treatment, significant enhancement of microbicidal activity was observed. When the aBL is added before the TCs, there was equivalent bactericidal effect, indicating that TCs primary action against biofilms were not as photosensitizers. These findings suggest that aBL can significantly enhance the antimicrobial activity of TCs, potentially offering a new effective approach to treating biofilm-associated infections and combating AMR when aBL is applicable. Full article
(This article belongs to the Special Issue Novel Insights into the Biofilms)
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15 pages, 3673 KiB  
Article
The Effect of Xanthohumol and Thymol on Candida albicans Filamentation and Its Impact on the Structure, Size, and Cell Viability of Biofilms Developed over Implant Surfaces
by Enrique Bravo, Marion Arce, David Herrera and Mariano Sanz
Cells 2024, 13(22), 1877; https://doi.org/10.3390/cells13221877 - 13 Nov 2024
Viewed by 1179
Abstract
The aim of this in vitro study was to evaluate the effect of xanthohumol and thymol on the impact of Candida albicans on the structure, size and cell viability of subgingival biofilms formed on dental implant surfaces. The structure and microbial biomass of [...] Read more.
The aim of this in vitro study was to evaluate the effect of xanthohumol and thymol on the impact of Candida albicans on the structure, size and cell viability of subgingival biofilms formed on dental implant surfaces. The structure and microbial biomass of biofilms developed after 72 h, treated and untreated with both extracts, were compared by scanning electron microscopy (SEM) and confocal laser microscopy (CLSM). Quantitative polymerase chain reaction (qPCR) was used to quantify the number of viable and total microorganisms of each of the biofilm-forming strains in each condition. A general linear model was used to compare and validate the CLSM and qPCR results. The presence of xanthohumol and thymol during biofilm development inhibited the filamentous growth of C. albicans. The biofilm incubated with xanthohumol had significantly lower bacterial biomass and cell viability than the biofilm not exposed to the extract (p < 0.05). In contrast, these global parameters showed no differences when the biofilm was incubated with thymol. In the presence of xanthohumol, there was a decrease in counts and cell viability of Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. Thymol treatment reduced the viability of F. nucleatum and P. gingivalis. The presence of these vegetable extracts during the development of a dynamic in vitro multispecies biofilm model inhibited the filamentous growth of C. albicans, partially reversing the effect that the fungus exerted on the structure, size and vitality of periodontopathogenic bacteria. Full article
(This article belongs to the Special Issue Novel Insights into the Biofilms)
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