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Guest Editor

Institute for Theoretical and Applied Electrodynamics, Russian Academy of Sciences, Moscow 125412, Russia
Interests: plasmonics; nanophotonics; sensors; SERS

Prof. Dr. Grégory Barbillon

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EPF-Ecole d'Ingénieurs (Graduate School of Engineering), 55 Avenue du Président Wilson, 94230 Cachan, France
Interests: plasmonics; sensors; nanophotonics; SERS
Special Issues, Collections and Topics in MDPI journals

Prof. Dr. Ilya Kurochkin

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Institute of Biochemical Physics, Russian Academy of Sciences, Moscow 119334, Russia
Interests: biotechnology; polymer composites; biophotonics; sensors

Special Issue Information

Dear Colleagues,

SARS-CoV-2 is responsible for the ongoing outbreak of atypical pneumonia (COVID-2019) that has affected nearly 490 million people and killed over 6 million in more than 220 countries. The development of a highly sensitive and specific spectral method for the rapid detection of viruses, particularly SARS-CoV-2 strains, could reduce both the time and cost of detection, ensure earlier diagnosis, and save many lives. We are soliciting papers on nanomaterial-based surface-enhanced Raman scattering (SERS) spectroscopy, which has the capability for very low-level fingerprint identification of virus, antigen, and antibody via metasurfaces, plasmonic nanoparticles, and heterostructure material. Papers devoted to any other spectroscopes and photonic methods of SARS-CoV-2 sensing are most welcome.

Prof. Dr. Andrey Sarychev
Prof. Dr. Grégory Barbillon
Prof. Dr. Ilya Kurochkin
Guest Editors

Manuscript Submission Information

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Keywords

SARS-CoV-2
nanomaterial
SERS
fluorescence
spike protein
plasmonic nanostructures
aptasensor

Published Papers (2 papers)

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Research

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Open AccessArticle

Development of a Single-Chain Variable Fragment of CR3022 for a Plasmonic-Based Biosensor Targeting the SARS-CoV-2 Spike Protein

Taufik Ramdani Tohari

and

Biosensors 2022, 12(12), 1133; https://doi.org/10.3390/bios12121133 - 06 Dec 2022

Cited by 1 | Viewed by 1107

Abstract

Two years after SARS-CoV-2 caused the first case of COVID-19, we are now in the “new normal” period, where people’s activity has bounced back, followed by the easing of travel policy restrictions. The lesson learned is that the wide availability of accurate and rapid testing procedures is crucial to overcome possible outbreaks in the future. Therefore, many laboratories worldwide have been racing to develop a new point-of-care diagnostic test. To aid continuous innovation, we developed a plasmonic-based biosensor designed explicitly for portable Surface Plasmon Resonance (SPR). In this study, we designed a single chain variable fragment (scFv) from the CR3022 antibody with a particular linker that inserted a cysteine residue at the second position. It caused the linker to have a strong affinity to the gold surface through thiol-coupling and possibly become a ready-to-use bioreceptor toward a portable SPR gold chip without purification steps. The theoretical affinity of this scFv on spike protein was −64.7 kcal/mol, computed using the Molecular Mechanics Generalized Born Surface Area (MM/GBSA) method from the 100 ns molecular dynamics trajectory. Furthermore, the scFv was produced in Escherichia coli BL21 (DE3) as a soluble protein. The binding activity toward Spike Receptor Binding Domain (RBD) SARS-CoV-2 was confirmed with a spot-test, and the experimental binding free energy of −10.82 kcal/mol was determined using portable SPR spectroscopy. We hope this study will be useful in designing specific and low-cost bioreceptors, particularly early in an outbreak when the information on antibody capture is still limited. Full article

(This article belongs to the Special Issue Nanophotonics and Plasmonics for SARS-CoV-2 Biosensing)

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Review

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Open AccessReview

Recent Advances of Representative Optical Biosensors for Rapid and Sensitive Diagnostics of SARS-CoV-2

and

Biosensors 2022, 12(10), 862; https://doi.org/10.3390/bios12100862 - 12 Oct 2022

Cited by 3 | Viewed by 1201

Abstract

The outbreak of Corona Virus Disease 2019 (COVID-19) has again emphasized the significance of developing rapid and highly sensitive testing tools for quickly identifying infected patients. Although the current reverse transcription polymerase chain reaction (RT-PCR) diagnostic techniques can satisfy the required sensitivity and specificity, the inherent disadvantages with time-consuming, sophisticated equipment and professional operators limit its application scopes. Compared with traditional detection techniques, optical biosensors based on nanomaterials/nanostructures have received much interest in the detection of SARS-CoV-2 due to the high sensitivity, high accuracy, and fast response. In this review, the research progress on optical biosensors in SARS-CoV-2 diagnosis, including fluorescence biosensors, colorimetric biosensors, Surface Enhancement Raman Scattering (SERS) biosensors, and Surface Plasmon Resonance (SPR) biosensors, was comprehensively summarized. Further, promising strategies to improve optical biosensors are also explained. Optical biosensors can not only realize the rapid detection of SARS-CoV-2 but also be applied to judge the infectiousness of the virus and guide the choice of SARS-CoV-2 vaccines, showing enormous potential to become point-of-care detection tools for the timely control of the pandemic. Full article

(This article belongs to the Special Issue Nanophotonics and Plasmonics for SARS-CoV-2 Biosensing)

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