Microfluidics and Microscale Biological Analysis

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: 31 December 2026 | Viewed by 2029

Special Issue Editors

School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao, China
Interests: microfluidics; single cell analysis; organ-on-a-chip; biofabrication

E-Mail Website
Guest Editor
Department of Applied Science and Technology (DISAT), Polytechnic University of Turin, Torino, Italy
Interests: microfluidics; biosensors; point-of-care testing; biomedical diagnostics

E-Mail Website
Guest Editor
School of Health and Life Sciences, University of Health and Rehabilitation Sciences, Qingdao, China
Interests: microfluidics; biosensors; point-of-care testing; digital microfluidics; biomedical diagnostics

Special Issue Information

Dear Colleagues,

We are pleased to invite you to contribute to the Special Issue “Microfluidics and Microscale Biological Analysis” in the journal Biosensors (MDPI).

This Special Issue aims to highlight recent advances and innovative methodologies at the intersection of microfluidics, miniaturized biosensing and microscale analysis. It will provide a platform for sharing cutting-edge research on the design, fabrication and application of integrated microsystems that enable precise manipulation, processing and analysis of biological samples at the micro- and nano-scale.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following:

  1. Microfluidic chip design and fabrication;
  2. Micro-scale biological sample processing and preparation;
  3. Single-cell analysis and manipulation;
  4. Integrated micro/nano sensors and detection technologies;
  5. Biomolecular handling and analysis within micro-environments;
  6. System integration and automation for micro/nanofluidic platforms.

We look forward to receiving your contributions.

Dr. Jiu Deng
Dr. Peng Zhang
Dr. Shuai Yuan
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microfluidics
  • biosensors
  • lab-on-a-chip
  • single-cell analysis
  • point-of-care testing
  • digital microfluidics
  • biomedical diagnostics

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Published Papers (1 paper)

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Research

14 pages, 6393 KB  
Article
Droplet-Interlaced Generator with On-Chip Metal–Liquid Micromirrors for Enhanced Microfluidic Absorbance Detection
by Haobo Liu, Laidi Jin, Zehang Gao, Chuanjin Cui, Yongjie Yu, Fei Deng, Xiuli Gao, Jianlong Zhao, Shengtai Bian and Shilun Feng
Biosensors 2026, 16(4), 202; https://doi.org/10.3390/bios16040202 - 2 Apr 2026
Viewed by 691
Abstract
Droplet microfluidics has been widely used in biological, chemical, and medical research owing to its advantages of miniaturization, high throughput, and low reagent consumption. However, limited sensitivity and optical path length in on-chip absorbance detection remain major challenges for droplet-based microfluidic analysis. Traditional [...] Read more.
Droplet microfluidics has been widely used in biological, chemical, and medical research owing to its advantages of miniaturization, high throughput, and low reagent consumption. However, limited sensitivity and optical path length in on-chip absorbance detection remain major challenges for droplet-based microfluidic analysis. Traditional absorbance detection suffers from low sensitivity due to the extremely short optical path in microfluidic channels, while existing optical path extension methods have drawbacks such as complex fabrication, easy droplet rupture, or strict incident angle requirements. To address these issues, this study developed a droplet microfluidic absorbance detection platform integrating optical fibers, on-chip micromirrors, external fluidic actuation, and an absorbance detection module. Microchannel sidewalls filled with low-melting-point metal act as mirrors; the multi-reflection optical path, combined with optical fibers and micromirrors, compensates for insufficient light manipulation and effectively extends the absorption path length, improving sensitivity and accuracy. Using this method, the detection limit for methylene blue solution was 20 μM, and the sensitivity for Escherichia coli (E. coli) suspension was doubled compared with traditional Nanodrop OD600 measurement. This device features low fabrication difficulty and cost and stable detection, providing a proof-of-concept strategy for enhanced absorbance detection in droplet microfluidic systems. Full article
(This article belongs to the Special Issue Microfluidics and Microscale Biological Analysis)
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