Advances in Label-Free Electrochemical Biosensing: From Surface Engineering to Health Monitoring Devices

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 1 November 2026 | Viewed by 373

Editors


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Guest Editor
Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, ON M5S 3H6, Canada
Interests: electrochemical sensors; biosensors; point-of-care diagnostic devices; surface chemistry; nanotechnology
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Department of Mechanical and Materials Engineering, The University of Western Ontario, London, ON N6A 5B9, Canada
Interests: carbon based electronics; bioelectronics; laser material processing; wearable flexible sensors; medical device design
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The rapid evolution of electrochemical biosensing technologies has created new opportunities for translating fundamental discoveries into practical, real-world solutions. Label-free electrochemical strategies play a pivotal role in this progress, as they simplify sensor fabrication, reduce costs, and enable streamlined, real-time measurements. In particular, advances in surface engineering have transformed the performance and reliability of biosensors, while the development of wearable and health monitoring devices highlights their growing societal impact.

This Special Issue aims to bridge fundamental innovations in surface chemistry and interface design with applied technologies for health and beyond. We welcome contributions that expand the scientific frontiers of label-free electrochemical biosensing and demonstrate their potential for scalable, cost-effective, and sustainable deployment.

We invite original research articles and reviews on, but not limited to, the following topics:

  • Advanced surface engineering and biointerface strategies for enhanced sensor performance;
  • Nanomaterial-enabled label-free sensor architectures;
  • Sustainable and eco-friendly fabrication methods;
  • Multiplexed and high-throughput biosensing platforms;
  • Wearable, implantable, and flexible label-free electrochemical devices;
  • Real-time health monitoring (in vitro, in vivo, and point-of-care);
  • Microfluidic integration with label-free platforms;
  • Machine learning and AI for data processing and optimization;
  • Wireless, IoT-enabled, and next-generation connected biosensors.

Dr. Soha Ahmadi
Prof. Dr. George Karl Knopf
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrochemical biosensors
  • label-free electrochemical biosensors
  • surface engineering
  • biointerfaces
  • biorecognition
  • nanomaterials
  • real-time monitoring
  • multiplexed biosensing
  • point-of-care devices
  • wearable and flexible biosensors
  • health monitoring devices
  • microfluidic integration
  • sustainable and eco-friendly materials
  • machine learning and artificial intelligence
  • wireless and IoT-enabled sensors

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Published Papers (1 paper)

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Research

22 pages, 7063 KB  
Article
Quantitative Loop-Mediated Isothermal Amplification (qLAMP) for the Rapid Discrimination of Normal and Cancerous Tissue Models: An Arduino-Based Portable Cancer Detection System Assisted by a pH Microelectrode
by Sergio Bravo-González, Luisa María Reyes-Cortés, Kristen Aideé Pérez-Alvarez, Grissel Trujillo-de Santiago and Mario Moisés Álvarez
Biosensors 2026, 16(8), 453; https://doi.org/10.3390/bios16080453 - 20 Aug 2026
Abstract
Cancer, the second leading cause of death worldwide, is a significant global challenge, and widespread, accessible, and early diagnostics are recognized as the most cost-effective strategies for reducing cancer burdens. Point-of-care (POC) systems offer an attractive alternative by enabling rapid and cost-effective diagnoses. [...] Read more.
Cancer, the second leading cause of death worldwide, is a significant global challenge, and widespread, accessible, and early diagnostics are recognized as the most cost-effective strategies for reducing cancer burdens. Point-of-care (POC) systems offer an attractive alternative by enabling rapid and cost-effective diagnoses. We introduce a novel POC strategy for cancer biomarker identification based on monitoring the isothermal amplification of relevant cancer markers using a portable Arduino-based loop-mediated isothermal amplification (LAMP) system. The trajectory of the LAMP reaction during the first 3 min of the reaction is used as an indicator of the rate of amplification (defined as the mP3 value). We obtained sets of mP3 values that showed statistically significant differences in the genetic expression of four genes (ESR 1, PGR, Her2, and Ki67) within and between tissue spheroids derived from the MCF7, MDA-MB-231, Du145, and BJ fibroblast cell lines. We then used principal component analysis and clustering techniques to demonstrate that the mP3 value sets derived from the expression of the four selected genes are sufficient to distinguish tissue spheroids derived from four different commercial cell lines. Further qPCR and immunostaining assays confirmed the quantitative LAMP (qLAMP) experimental trends. The immunostaining results were consistent with previous literature reports and with our qLAMP and qPCR results. We present a proof-of-concept demonstration of the use of a LAMP-based POC platform for the identification or discrimination of cancer tissues. Our strategy can be extended to other diseases associated with altered gene expression in body tissues or fluids. Full article
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