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Biosensors
Special Issues
Immunoassays and Biosensing (2nd Edition)
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Immunoassays and Biosensing (2nd Edition)

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 2339

Special Issue Editors

Prof. Dr. Dongyang Li

E-Mail Website
Guest Editor
College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China
Interests: nanobodies; immunoassays; agro-product safety; food safety; public health
Special Issues, Collections and Topics in MDPI journals
Dr. Jiukai Zhang

E-Mail Website
Guest Editor
Chinese Academy of Inspection and Quarantine, Beijing 100176, China
Interests: food authentication; metabolomics; LC-MS; GC-MS
Special Issues, Collections and Topics in MDPI journals
Dr. Maojun Jin

E-Mail Website
Guest Editor
Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences(CAAS), Beijing, China
Interests: food quality and safety; immunoassay; trace detection
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Immunoassays and biosensors have been extensively employed in various fields, including clinical, food safety, and environmental monitoring. Immunoassays are continuously being developed in order to produce simple, rapid, field-portable tests that may be either qualitative or quantitative in nature, especially in relation to the outbreak of the COVID-19 pandemic. The application of new biorecognition elements and novel transducers has also led to a rapid increase in publications concerning the development of biosensors. However, further innovations are required to strengthen the use of immunoassays and biosensors for numerous practical applications; these developments include, but are not limited to, antibodies; new biomaterials; signal-amplification strategies; bio-conjugate techniques; sample pre-treatment methods; automation; and multiplex detection.

This Special Issue aims to collect original articles and reviews addressing the fabrication, manufacturing, innovative application, challenges, and future prospects of immunoassays and biosensors in various fields, including medical diagnostics, public health, environmental monitoring, agriculture, and food.

Prof. Dr. Dongyang Li
Dr. Jiukai Zhang
Dr. Maojun Jin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • immunoassay
  • biosensor
  • antibody
  • nanobody
  • nanoparticle
  • magnetic beads
  • digital ELISA
  • phage display

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Related Special Issue

Published Papers (2 papers)

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Review

35 pages, 3811 KiB  
Review
Nanobody-Based Immunoassays for the Detection of Food Hazards—A Review
by Wenkai Li, Zhihao Xu, Qiyi He, Junkang Pan, Yijia Zhang, El-Sayed A. El-Sheikh, Bruce D. Hammock and Dongyang Li
Biosensors 2025, 15(3), 183; https://doi.org/10.3390/bios15030183 - 13 Mar 2025
Viewed by 668
Abstract
Food safety remains a significant global challenge that affects human health. Various hazards, including microbiological and chemical threats, can compromise food safety throughout the supply chain. To address food safety issues and ensure public health, it is necessary to adopt rapid, accurate, and [...] Read more.
Food safety remains a significant global challenge that affects human health. Various hazards, including microbiological and chemical threats, can compromise food safety throughout the supply chain. To address food safety issues and ensure public health, it is necessary to adopt rapid, accurate, and highly specific detection methods. Immunoassays are considered to be an effective method for the detection of highly sensitive biochemical indicators and provide an efficient platform for the identification of food hazards. In immunoassays, antibodies function as the primary recognition elements. Nanobodies have significant potential as valuable biomolecules in diagnostic applications. Their distinctive physicochemical and structural characteristics make them excellent candidates for the development of reliable diagnostic assays, and as promising alternatives to monoclonal and polyclonal antibodies. Herein, we summarize a comprehensive overview of the status and prospects of nanobody-based immunoassays in ensuring food safety. First, we begin with a historical perspective on the development of nanobodies and their unique characteristics. Subsequently, we explore the definitions and boundaries of immunoassays and immunosensors, before discussing the potential applications of nanobody-based immunoassays in food safety testing that have emerged over the past five years, and follow the different immunoassays, highlighting their advantages over traditional detection methods. Finally, the directions and challenges of nanobody-based immunoassays in food safety are discussed. Due to their remarkable sensitivity, specificity and versatility, nanobody-based immunoassays hold great promise in revolutionizing food safety testing and ensuring public health and well-being. Full article
(This article belongs to the Special Issue Immunoassays and Biosensing (2nd Edition))
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23 pages, 4810 KiB  
Review
Dual Biomarker Strategies for Liquid Biopsy: Integrating Circulating Tumor Cells and Circulating Tumor DNA for Enhanced Tumor Monitoring
by Ga Young Moon, Basak Dalkiran, Hyun Sung Park, Dongjun Shin, Chaeyeon Son, Jung Hyun Choi, Seha Bang, Hosu Lee, Il Doh, Dong Hyung Kim, Woo-jin Jeong and Jiyoon Bu
Biosensors 2025, 15(2), 74; https://doi.org/10.3390/bios15020074 - 28 Jan 2025
Cited by 1 | Viewed by 1226
Abstract
The liquid biopsy has gained significant attention in cancer diagnostics, with circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) being recognized as key biomarkers for tumor detection and monitoring. However, each biomarker possesses inherent limitations that restrict its standalone clinical utility, such [...] Read more.
The liquid biopsy has gained significant attention in cancer diagnostics, with circulating tumor cells (CTCs) and circulating tumor DNA (ctDNA) being recognized as key biomarkers for tumor detection and monitoring. However, each biomarker possesses inherent limitations that restrict its standalone clinical utility, such as the rarity and heterogeneity of CTCs and the variable sensitivity and specificity of ctDNA assays. This highlights the necessity of integrating both biomarkers to maximize diagnostic and prognostic potential, offering a more comprehensive understanding of the tumor biology and therapeutic response. In this review, we summarize clinical studies that have explored the combined analysis of CTCs and ctDNA as biomarkers, providing insights into their synergistic value in diverse tumor types. Specifically, this paper examines the individual advantages and limitations of CTCs and ctDNA, details the findings of combined biomarker studies across various cancers, highlights the benefits of dual biomarker approaches over single-biomarker strategies, and discusses future prospects for advancing personalized oncology through liquid biopsies. By offering a comprehensive overview of clinical studies combining CTCs and ctDNA, this review serves as a guideline for researchers and clinicians aiming to enhance biomarker-based strategies in oncology and informs biosensor design for improved biomarker detection. Full article
(This article belongs to the Special Issue Immunoassays and Biosensing (2nd Edition))
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