Biosensors for Sensitive and Rapid Detection

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor and Bioelectronic Devices".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 1518

Special Issue Editors


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Guest Editor
School of Life and Health Technology, Dongguan University of Technology, Dongguan 523808, China
Interests: biosensor; DNA nanotechnology; molecular logic gate; food safety; environmental monitoring

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Guest Editor
Institute of Nanoscience and Nanotechnology, NCSR “Demokritos”, 15341 Aghia Paraskevi, Greece
Interests: microfluidics; lab-on-PCB; biochips; point-of-care; plasma surface engineering
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Special Issue Information

Dear Colleagues,

Biosensors provide an ideal tool for detecting hazardous substances and biomarkers. Compared with detection methods using bulky instruments, biosensors can achieve the simple and rapid detection of targets. In actual samples, biomarkers and pollutants often exist in trace concentrations, which increases the difficulty of biosensor analysis. In order to achieve sensitive detection, it is often necessary to introduce signal amplification strategies when designing biosensors. Another advantage of biosensors is their rapid response, which facilitates on-site detection. Combining the advantages of simple operation, high sensitivity, and good specificity, biosensors have played an important role in point-of-care applications. This Special Issue focuses on biosensors and their applications in the sensitive and rapid detection of a series of targets. Original articles and reviews are welcome.

Research areas may include (but are not limited to) the following:

  1. Biosensors with high sensitivity for the detection of DNA, RNA, heavy metals, antibiotic residue, pesticide residue, mycotoxin, microplastic, endocrine-disrupting chemicals, etc.
  2. Biosensor applications in disease diagnosis, food safety, and environmental monitoring.
  3. Electrochemical, fluorescent, colorimetric, strip, and SPR biosensors in the sensitive and rapid detection of targets.
  4. Molecular logic gate biosensors to realize intelligent detection.

I look forward to receiving your contributions.

Dr. Junhua Chen
Dr. Angeliki Tserepi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biosensor
  • signal amplification
  • on-site detection
  • disease diagnosis
  • food safety
  • environmental monitoring
  • pollutant
  • molecular logic gate

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Published Papers (2 papers)

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Research

11 pages, 3631 KB  
Article
Size Enlargement Enabled Functional Profiling of Extracellular Vesicle at Single-Particle Level
by Jia Yao, Xianyue Ji, Xingyu Tao, Ziyan Li, Shao Su and Xianguang Ding
Biosensors 2026, 16(4), 230; https://doi.org/10.3390/bios16040230 - 21 Apr 2026
Viewed by 594
Abstract
Extracellular vesicles (EVs) are promising biomarkers for liquid biopsy, but their clinical application is limited by intrinsic heterogeneity and the lack of methods capable of resolving functionally distinct EV subpopulations at the single-vesicle level. Conventional bulk analyses obscure rare but clinically relevant EV [...] Read more.
Extracellular vesicles (EVs) are promising biomarkers for liquid biopsy, but their clinical application is limited by intrinsic heterogeneity and the lack of methods capable of resolving functionally distinct EV subpopulations at the single-vesicle level. Conventional bulk analyses obscure rare but clinically relevant EV subsets, while most single-EV approaches focus on physical properties or surface markers, with limited access to intravesicular functional information. Here, we report a fusion-enabled EV detection strategy at the single-particle level for functional profiling of macrophage-derived EVs. Liposomal probes encapsulating L-arginine, NADPH, and a nitric oxide (NO)-responsive fluorescent dye are engineered to fuse with EV membranes, delivering substrates into the vesicle lumen. In macrophage-derived EVs, inducible nitric oxide synthase (iNOS) catalyzes NO production, activating the fluorescent probe and generating a localized signal within individual vesicles. Signal generation is confined to vesicle-restricted reactions, ensuring specificity and minimizing background. The formation of hybrid vesicles further facilitates optical detection using conventional fluorescence microscopy. Full article
(This article belongs to the Special Issue Biosensors for Sensitive and Rapid Detection)
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20 pages, 2684 KB  
Article
Enhancing the Detection of Long-Chain Aldehydes by Peptide-Based Biosensors Through Counter-Ion Exchange
by Tomasz Wasilewski, Damian Neubauer, Elisabete Fernandes, Rafał Kiejzik, Bartosz Szulczyński, Jacek Gębicki, Wojciech Kamysz and Marek Wojciechowski
Biosensors 2026, 16(3), 162; https://doi.org/10.3390/bios16030162 - 13 Mar 2026
Viewed by 633
Abstract
Long-chain aldehydes, particularly nonanal, are recognized as potential volatile biomarkers of lung cancer in exhaled breath. This study investigates the influence of peptide counter-ions on the performance of QCM-based biosensors using two odorant-binding protein-derived peptides (OBPP4 and OBPP4 GSGSGS) for the selective gas-phase [...] Read more.
Long-chain aldehydes, particularly nonanal, are recognized as potential volatile biomarkers of lung cancer in exhaled breath. This study investigates the influence of peptide counter-ions on the performance of QCM-based biosensors using two odorant-binding protein-derived peptides (OBPP4 and OBPP4 GSGSGS) for the selective gas-phase detection of these aldehydes. Exchanging the counter-ion from trifluoroacetate to chloride improves biosensor sensitivity and lowers the limit of detection within the set of biosensors investigated in this study. The OBPP4 GSGSGS with chloride exhibited the highest sensitivity to nonanal (0.153 Hz/ppm) and the lowest LOD (9.8 ppm), with excellent selectivity over other groups of volatiles. The novelty of this work lies in demonstrating, for the first time, that simple counter-ion exchange in synthetic peptides can significantly enhance the gas-phase binding of volatile aldehydes, classified as lung cancer biomarkers, without altering the peptide sequence, offering a straightforward and effective optimization strategy for peptide-based piezoelectric biosensors. Full article
(This article belongs to the Special Issue Biosensors for Sensitive and Rapid Detection)
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