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Biosensors
Special Issues
Point-of-Care Testing Using Biochemical Sensors for Health and Safety
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Point-of-Care Testing Using Biochemical Sensors for Health and Safety

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 1036

Special Issue Editors

Dr. Jiangjiang Zhang

E-Mail Website
Guest Editor
1. School of Life Science, Beijing Institute of Technology, No. 5 Zhongguancun South Street, Beijing 100081, China
2. Zhengzhou Research Institute, Beijing Institute of Technology, Zhengzhou 450000, China
Interests: biosensors; point-of-care tests; immunoassays; serodiagnostics; gold nanomaterials; fluorescent nanomaterials; metal–organic frameworks; hydrogel film sensors
Prof. Dr. Xiaolin Huang

E-Mail Website
Guest Editor
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
Interests: food safety detection; immunoassay; biosensor; nanomaterials; in vitro diagnostic
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,  

Biochemical sensors used for point-of-care testing (POCT) have the excellent advantages of convenience, high efficiency, and a low cost for onsite detection, while being easy to operate and enabling large-scale screening and at-home care. As such, they have been used in ensuring food/public safety, epidemic surveillance, disease diagnosis/prognosis monitoring, etc. Coupling POCT techniques, which use a naked-eye readable signal or miniaturized sensing device, with smartphone-based intelligent detections is a promising area of research and one of the most important research fields currently under development. These systems offer a primary diagnosis without a time delay and are also perfectly suitable for long-term monitoring without the need for making appointments with doctors or experts.

This Special Issue aims to present research articles and reviews/mini-reviews detailing the recent advances in POCT sensing systems. Topics of interest for this Special Issue include, but are not limited to, the following:

  • Lateral flow assays/microfluidic assays/wearable sensors, etc;
  • Molecular/nanomaterial-based colorimetric/fluorescent/spectrometric/electrochemical assays;
  • POCT sensors for disease biomarkers/drugs/toxics/antibiotics/pathogens;
  • Smartphone-based automatic and intelligent assays;
  • Integrated multiplex assays.

Dr. Jiangjiang Zhang
Prof. Dr. Xiaolin Huang
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • biochemical sensors
  • point-of-care tests
  • disease biomarker detections
  • molecular diagnosis
  • public/food safety
  • immunoassays
  • lateral flow assays
  • microfluidics
  • wearable sensors

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Published Papers (3 papers)

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Research

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18 pages, 1867 KB  
Article
Rapid Visual Detection of Mycoplasma Hominis Using an RPA-CRISPR/Cas12a Assay
by Jie Chen, Shutao Liu, Sunyi Chen, Jingwen Mai, Maiwula Abudukadi, Yao Chen, Jie Lu, Guanglei Li and Chenchen Ge
Biosensors 2025, 15(12), 821; https://doi.org/10.3390/bios15120821 - 18 Dec 2025
Abstract
Mycoplasma hominis (MH) is a prevalent opportunistic pathogen that is strongly associated with a wide range of urogenital tract infections and severe adverse pregnancy outcomes in clinical settings. Current MH detection methods, including microbial culture and qPCR, are time-consuming and rely on complex [...] Read more.
Mycoplasma hominis (MH) is a prevalent opportunistic pathogen that is strongly associated with a wide range of urogenital tract infections and severe adverse pregnancy outcomes in clinical settings. Current MH detection methods, including microbial culture and qPCR, are time-consuming and rely on complex equipment, making them unsuitable for scenarios requiring rapid or simplified testing. In this study, we developed a visual readout biosensing platform by synergistically integrating recombinase polymerase amplification (RPA), CRISPR/Cas12a-mediated target nucleic acid recognition, and lateral flow biosensors for the rapid, sensitive, and specific identification of MH. The assay specifically targets the MH-specific 16S rRNA gene, achieving a limit of detection as low as 2 copies/reaction of recombinant plasmid containing the target gene with a total assay time of 60 min. Critical reaction parameters, including Cas12a-crRNA molar ratio, volume of RPA amplicon input, and Cas12a cleavage time, were systematically optimized to maximize the biosensor’s response efficiency and detection reliability. The platform exhibited exceptional specificity, with no cross-reactivity observed against common co-occurring urogenital pathogens, and effectively minimized aerosol contamination risks via a rigorous decontamination workflow. Furthermore, this work represents the first documented implementation of a contamination-control protocol for an MH-specific CRISPR-LFA assay. Notably, testing results from 18 clinical samples demonstrated the high specificity of this assay, highlighting its promising potential for clinical application. Full article
(This article belongs to the Special Issue Point-of-Care Testing Using Biochemical Sensors for Health and Safety)
13 pages, 3528 KB  
Article
Urinary Metabolomic Changes and Potential Exercise-Induced Muscle Damage Biomarkers Identification in Trained Young Males Following Acute Intermittent Rowing Training
by Yang Cheng, Yue Yi, Xuefeng Shi and Shumin Bo
Biosensors 2025, 15(12), 803; https://doi.org/10.3390/bios15120803 - 8 Dec 2025
Viewed by 315
Abstract
(1) Background: This study aims to explore the changes in urinary metabolomic profile among trained young males following acute intermittent rowing training (AIRT), and to identify potential urinary biomarkers associated with exercise-induced muscle damage (EIMD). (2) Methods: 22 trained young males were recruited [...] Read more.
(1) Background: This study aims to explore the changes in urinary metabolomic profile among trained young males following acute intermittent rowing training (AIRT), and to identify potential urinary biomarkers associated with exercise-induced muscle damage (EIMD). (2) Methods: 22 trained young males were recruited to perform AIRT. The changes in blood biochemical indexes associated with EIMD were analyzed. EIMD occurrence was evaluated using blood biochemical indexes, muscle function, and pain assessment. The changes in urinary metabolites were determined using untargeted metabolomic analysis. (3) Results: Four blood biochemical indices, including creatine kinase, lactate dehydrogenase, creatine kinase-MB, and hydroxybutyrate dehydrogenase, were significantly elevated immediately after AIRT. Furthermore, an obvious immune response appeared, and countermovement jump performance significantly decreased. Among 384 urinary metabolites, 33 were significantly upregulated, and 12 were downregulated immediately after AIRT. Upregulated metabolites were mainly involved in phenylacetate metabolism, ammonia recycling, the urea cycle, and glutathione metabolism. Four potential urinary biomarkers were identified, including 2′-Deoxycytidine, cytosine, Phenylacetaldehyde, and Pyridoxamine. (4) Conclusions: AIRT induced EIMD in all participants and significantly altered urinary metabolite profiles. The changes in urinary metabolites and pathways were due to the metabolic adaptation to oxidative stress, inflammatory responses, and ammonia metabolism imbalance. The selected four potential urinary biomarkers provide important evidence for the further development of a non-invasive, urine-based method for the immediate assessment of EIMD. Full article
(This article belongs to the Special Issue Point-of-Care Testing Using Biochemical Sensors for Health and Safety)
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Review

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19 pages, 2107 KB  
Review
Recent Advances in MXene-Based Screen-Printed Electrochemical Sensors for Point-of-Care Biomarker Detections
by Thao Thi Nguyen, Liang Zhou, Jinming Kong, Aiqin Luo, Zikai Hao and Jiangjiang Zhang
Biosensors 2025, 15(12), 804; https://doi.org/10.3390/bios15120804 - 8 Dec 2025
Viewed by 424
Abstract
Contemporary biomedical diagnostics increasingly demand high sensitivity for pathogen detection and real-time health monitoring. In response to these requirements, screen-printed electrochemical sensors (SPEs) have emerged as a practical analytical platform owing to their low cost, portability, and compatibility with point-of-care and wearable systems. [...] Read more.
Contemporary biomedical diagnostics increasingly demand high sensitivity for pathogen detection and real-time health monitoring. In response to these requirements, screen-printed electrochemical sensors (SPEs) have emerged as a practical analytical platform owing to their low cost, portability, and compatibility with point-of-care and wearable systems. In the recent past, nanomaterials in two-dimensional format, especially MXenes, have gained much interest due to their high electrical conductivity, controllable surface chemistry, and biocompatibility, which can improve the performance and applicability of SPEs. The current review concentrates on the latest developments between 2020 and 2025, providing a critical assessment of research employing MXene-based nanomaterials for the modification and development of screen-printed electrode platforms. We provide an overview of fabrication techniques, printing methods, and surface modification methods, and proceed with an analysis of the electrochemical performance of MXenes and MXene-based heterostructures. Lastly, contemporary issues are considered, and opinions are suggested to facilitate the translation of MXene-functionalized SPEs to real biomedical diagnosis solutions. Full article
(This article belongs to the Special Issue Point-of-Care Testing Using Biochemical Sensors for Health and Safety)
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