Design and Application of Microfluidic Biosensors in Biomedicine—2nd Edition

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 31 December 2026 | Viewed by 1695

Special Issue Editors


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Guest Editor
Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, CA 94158, USA
Interests: droplet; single-cell analysis; ScDNAseq; ScRNAseq; biomaterials; microfluidics commercialization
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Guest Editor
Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
Interests: droplet; microparticles; microfluidics; integrated systems; organ-on-a-chip; microvasculature
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Guest Editor
College of Mechanical Engineering, Jiangsu University of Science and Technology, Zhenjiang 212100, China
Interests: microfluidics; cell mechanics; cancer metastasis; laser processing
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Special Issue Information

Dear Colleagues,

Following the successful publication of the first edition, the Editorial Board, in collaboration with Guest Editors Dr. Hangrui Liu, Dr. Tianlong Zhang, and Dr. Yuxin Zhang, is pleased to announce the launch of the second edition of this Special Issue. This continuation seeks to further disseminate cutting-edge research and significant advancements at the intersection of microfluidics, biosensing, and biomedical applications.

The field is currently characterized by two predominant, synergistic trends aimed at enhancing analytical performance. First, there is a concerted effort to integrate novel functional nanostructures with innovative transduction principles. This convergence enables the creation of biosensing platforms with superior sensitivity, selectivity, and multiplexing capabilities. Second, significant research is directed toward the seamless integration of on-chip microfluidic separation or preprocessing units with detection modules. This holistic "sample-to-answer" strategy is crucial for developing robust, automated biosensing systems applicable to complex biological matrices. Consequently, research increasingly focuses on the rational design and fabrication of advanced materials and structures—with tailored optical, hydrophobic, electronic, or metallic properties—to facilitate the highly specific and sensitive detection of low-abundance biomarkers.

The second edition of this Special Issue will encompass a broad spectrum of topics spanning the design, fabrication, and characterization of novel micro/nanostructures for sensing; the development of advanced microfluidic techniques for particle/cell separation and sample preparation; the exploration of versatile optical, electrochemical, and mechanical sensing modalities for multiplexed detection on integrated platforms; the synthesis and functionalization of biological and bio-inspired materials for enhanced interfaces; the identification and validation of novel diagnostic biomarkers; and the critical translation of these biosensor technologies toward real-world clinical, environmental, and point-of-care applications. Through high-quality contributions, this Special Issue aims to advance both the fundamental understanding and practical deployment of microfluidic biosensors, thereby accelerating their impact on disease diagnosis, monitoring, and preventive healthcare. We cordially invite researchers to submit their original work on the abovementioned topics.

Dr. Hangrui Liu
Dr. Yuxin Zhang
Dr. Tianlong Zhang
Guest Editors

Manuscript Submission Information

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Keywords

  • biosensing techniques
  • micro/nano structures
  • pathogen detection
  • disease diagnosis
  • particle isolation/separation
  • single-cell analysis
  • point-of-care microdevices

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Published Papers (2 papers)

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Research

16 pages, 14520 KB  
Article
Tunable Particle Separation in a Straight Microchannel via Symmetrical Viscoelastic Sheath Flows
by Tianyuan Zhou, Qi Cui, Guizhong Tian, Jing Xia, Ping Liu, Yoichiroh Hosokawa, Yaxiaer Yalikun, Pan Wang, Shilun Feng and Tianlong Zhang
Biosensors 2026, 16(5), 273; https://doi.org/10.3390/bios16050273 - 8 May 2026
Viewed by 551
Abstract
In this study, we present a novel microfluidic platform for tunable size-based particle separation within a straight microchannel using symmetrical viscoelastic sheath flows. The device incorporates two pairs of symmetrical microchannels for sheath fluid injection: the first pair facilitates particle focusing and separation, [...] Read more.
In this study, we present a novel microfluidic platform for tunable size-based particle separation within a straight microchannel using symmetrical viscoelastic sheath flows. The device incorporates two pairs of symmetrical microchannels for sheath fluid injection: the first pair facilitates particle focusing and separation, while the second pair enables dynamic regulation of the separation distance between particle streams. Experimental results demonstrate that a 50 ppm polyethylene oxide (PEO) solution focuses 1 μm polystyrene particles toward the channel centerline via elastic forces, whereas 5 μm particles migrate toward the channel sidewalls under dominant inertial forces, effectively overcoming the elastic effects. The interplay between inertial and elastic forces thus achieves size-dependent particle separation. Furthermore, by adjusting the flow rate of the PEO sheath in the second pair of microchannels, the separation distance between the two particle populations can be modulated in real time. Higher PEO concentrations (500 and 1000 ppm) exhibit enhanced capabilities to deflect particle flow streams. By contrast, the lower PEO concentrations like 50, 100 and 200 ppm are more versatile in adjusting the separation distance. The biological applicability of this platform is further demonstrated through the tunable separation of Escherichia coli (E. coli) and Chlorella vulgaris (C. vulgaris). This microfluidic device demonstrates significant potential for downstream particle processing applications, including real-time particle detection and targeted drug delivery. Full article
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17 pages, 2735 KB  
Article
A Programmable and Portable Electromagnetic Microfluidic Platform for Droplet Manipulation
by Chaoze Xue, Shilun Feng, Wenshuai Wu, Zhe Zhang, Jianlong Zhao, Gaozhe Cai and Ting Zhou
Biosensors 2026, 16(4), 196; https://doi.org/10.3390/bios16040196 - 31 Mar 2026
Viewed by 813
Abstract
Droplet manipulation constitutes a fundamental operation in numerous bio-microfluidic applications, including but not limited to medical diagnostics and targeted drug delivery. Among the various technologies developed for this purpose, magnetic digital microfluidics (MDMF) has emerged as a compelling approach due to its inherent [...] Read more.
Droplet manipulation constitutes a fundamental operation in numerous bio-microfluidic applications, including but not limited to medical diagnostics and targeted drug delivery. Among the various technologies developed for this purpose, magnetic digital microfluidics (MDMF) has emerged as a compelling approach due to its inherent advantages of contamination-free actuation, low cost, and configurational flexibility. Nevertheless, conventional MDMF remains constrained by its reliance on bulky instrumentation and substantial power consumption for generating controllable magnetic fields, which limit its in-field applications. To address these limitations, this work presents a programmable and portable electromagnetic microfluidic droplet manipulation platform that synergistically integrates static and dynamic magnetic fields to enable non-contact, high-precision droplet control under ultra-low power conditions. The proposed system comprises an electromagnetic actuation module, a permanent magnet, and a glass substrate coated with Teflon film. The entire system is secured by a PMMA support structure, within which a glass substrate is mounted and spatially separated from the permanent magnet. The PMMA support is fabricated using a milling process, offering a simple manufacturing procedure and high structural reusability and reproducibility. The control logic is implemented on a field-programmable gate array (FPGA) development board, facilitating fully autonomous operation powered by a standard battery. The platform operates at a low voltage of 3.5 V and a driving current of 180 mA, corresponding to a total power consumption of merely 0.63 W, while achieving robust manipulation of droplets in the volume range of 0.5 to 5 μL. A maximum average droplet velocity of up to 0.6 cm/s was attained under optimal conditions. The proposed platform offers a scalable and energy-efficient solution for portable droplet-based assays and holds significant promise for integration into point-of-care diagnostic tools and field-ready biochemical analysis systems. The platform demonstrates excellent operational stability and reproducibility, as validated by repeated actuation experiments with a positioning deviation of approximately 0.1 mm under optimized conditions. The fabrication process also exhibits high reliability with consistent performance across multiple experimental runs. Full article
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