Biosensors for Biomedical Diagnostics

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensors and Healthcare".

Deadline for manuscript submissions: 31 March 2025 | Viewed by 2092

Special Issue Editor


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Medical School, Department of Clinical and Experimental Medicine, University of Sassari, 07100 Sassari, Italy
Interests: biosensor; biomedical applications; amperometry; biological fluids; interferents
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In 1962, the first enzyme-based biosensor was introduced. Since then, the development of biosensors has been remarkable. This diffusion is associated with a high sensitivity in monitoring, possibly in real time, as well as the possibility of miniaturization, which makes the biosensors applicable in different matrices, but above all the low production costs. The continuous development of biosensors concerns all aspects of their technology: the search for newer and more effect signal transduction methods; the use of more selective bio-elements towards the monitored analytes; and the use of polymers and containment networks in order to obtain ever greater selectivity and sensitivity, particularly in complex matrices such as biological samples. Biosensors have become a very important tool in diagnostics, drug discovery, and biomedicine as well.

Thus, the present Special Issue will mainly focus on possible new biosensor configurations, concentrating on biorecognition layers, transduction principles, and the use of new containment networks for the biosensor components, in relation to diagnostic applications for the recognition of important biomarkers in real samples.

Prof. Dr. Gaia Rocchitta
Guest Editor

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Keywords

  • enzyme biosensors
  • neurochemicals
  • drug discovery
  • diagnosis markers
  • biosensor development

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Published Papers (1 paper)

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Research

11 pages, 2604 KiB  
Article
Rapid Evaluation of Antibacterial Carbohydrates on a Microfluidic Chip Integrated with the Impedimetric Neoglycoprotein Biosensor
by Haijie Ji, Xueqiong Yang, Hang Zhou, Feiyun Cui and Qin Zhou
Biosensors 2023, 13(9), 887; https://doi.org/10.3390/bios13090887 - 18 Sep 2023
Cited by 1 | Viewed by 1354
Abstract
The colonization of some bacteria to their host cell is mediated by selective adhesion between adhesin and glycan. The evaluation of antiadhesive carbohydrates in vitro has great significance in discovering new antibacterial drugs. In this paper, a microfluidic chip integrated with impedimetric neoglycoprotein [...] Read more.
The colonization of some bacteria to their host cell is mediated by selective adhesion between adhesin and glycan. The evaluation of antiadhesive carbohydrates in vitro has great significance in discovering new antibacterial drugs. In this paper, a microfluidic chip integrated with impedimetric neoglycoprotein biosensors was developed to evaluate the antibacterial effect of carbohydrates. Mannosylated bovine serum albumin (Man-BSA) was taken as the neoglycoprotein and immobilized on the microelectrode-modified gold nanoparticles (Au NPs) to form a bionic glycoprotein nanosensing surface (Man-BSA/Au NPs). Salmonella typhimurium (S. typhimurium) was selected as a bacteria model owing to its selective adhesion to the mannose. Electrochemical impedance spectroscopy (EIS) was used to characterize the adhesion capacity of S. typhimurium to the Man-BSA/Au NPs and evaluate the antiadhesive efficacy of nine different carbohydrates. It was illustrated that the 4-methoxyphenyl-α-D-pyran mannoside (Phenyl-Man) and mannan peptide (Mannatide) showed excellent antiadhesive efficacy, with IC50 values of 0.086 mM and 0.094 mM, respectively. The microfluidic device developed in this study can be tested in multiple channels. Compared with traditional methods for evaluating the antibacterial drug in vitro, it has the advantages of being fast, convenient, and cost-effective. Full article
(This article belongs to the Special Issue Biosensors for Biomedical Diagnostics)
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