Biological Nitrogen Removal in the Multi-Omics Era: Coupling Anammox with Microbial-Mineral Synergy

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Microbiology".

Deadline for manuscript submissions: 10 May 2026 | Viewed by 721

Special Issue Editors


E-Mail Website
Guest Editor
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China
Interests: traditional biological; nitrogen removal technology; anaerobic ammonium oxidation
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
School of Environmental and Geographic Sciences, Qingdao University, Qingdao 266071, China
Interests: biological nutrient removal; functional microbial community; enhanced biological phosphorus removal; anammox coupling process; anaerobic fermentation; phosphorus recovery
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
National and Local & Joint Engineering Research Center for Urban Sewage Treatment and Resource Recycling, School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266520, China
Interests: sustainable nitrogen removal processes; anaerobic ammonium oxidation; microbi-al–mineral interactions; multi-omics microbial analysis; bioengineering in wastewater treatment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This study focuses on microorganism-centered technological strategies and bioengineering innovations to achieve efficient, stable, and sustainable nitrogen removal in complex wastewater environments. By deeply exploring microbial metabolic mechanisms, nitrogen transformation pathways, the development of novel microbial consortia, and process optimization approaches, this study seeks to promote the synergistic development of wastewater treatment systems for carbon reduction, resource recovery, and the removal of persistent emerging pollutants.

Research Directions:

  1. Mechanistic exploration of microbial nitrogen transformation;
  2. Applications of autotrophic nitrogen removal and anammox processes;
  3. Engineering optimization of aerobic granular sludge systems;
  4. Discovery of novel nitrogen-cycling microbial resources;
  5. Multi-omics analysis of nitrogen metabolism processes;
  6. Microbial–mineral interactions in nitrogen removal;
  7. Bioengineering strategies for system optimization;
  8. Practical applications and control strategies in wastewater treatment engineering;
  9. Detection, removal, and water quality assurance technologies for emerging pollutants.

You may choose our Joint Special Issue in Water.

Dr. Zhaoming Zheng
Dr. Xiaoxia Wang
Dr. Yuanyuan Miao
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biology is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • sustainable nitrogen removal processes
  • anaerobic ammonium oxidation
  • aerobic granular sludge
  • microbial–mineral interactions
  • multi-omics microbial analysis
  • bioengineering in wastewater treatment
  • nitrogen biogeo-chemical cycle
  • detection and removal of emerging pollutants
  • water quality assurance technologies
  • resource re-covery from wastewater

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

16 pages, 2104 KB  
Article
Construction of a Heterotrophic Nitrification–Aerobic Denitrification Composite Microbial Consortium and Its Bioaugmentation Role in Wastewater Treatment
by Wenjing Jiao, Haoyang Sun, Zixuan Zhang, Zuyin Xiao, Hanhan Song, Jiale Liu, Xiaole Xu, Juan Wang, Guiying Wang, Jiang Zhang, Chenyang Wang, Lusheng Li and Lifei Chen
Biology 2025, 14(12), 1734; https://doi.org/10.3390/biology14121734 - 4 Dec 2025
Viewed by 411
Abstract
Nitrogen pollution in wastewater remains a pressing environmental concern, prompting the need for efficient and sustainable treatment technologies. This study constructs an HN-AD microbial consortium using three pre-screened strains (Delftia tsuruhatensis SDU2, Pseudomonas stutzeri SDU10, Alcaligenes faecalis SDU20) and evaluates its bioaugmentation [...] Read more.
Nitrogen pollution in wastewater remains a pressing environmental concern, prompting the need for efficient and sustainable treatment technologies. This study constructs an HN-AD microbial consortium using three pre-screened strains (Delftia tsuruhatensis SDU2, Pseudomonas stutzeri SDU10, Alcaligenes faecalis SDU20) and evaluates its bioaugmentation effects in sequencing batch reactors (SBRs). An orthogonal test optimized the inoculation ratio as 2:3:3 (SDU2:SDU10:SDU20), achieving the highest ammonium removal efficiency of 96.02% in vitro. In SBR experiments, the bioaugmented reactor (SBR1) demonstrated superior nitrogen and organic matter removal compared to the control (SBR2). By day 40 of the enhancement phase, SBR1 achieved 88.9% ammonium removal and 93.7% COD removal, representing improvements of 20.5% and 17.9% over SBR2, respectively. Microbial community analysis revealed that bioaugmentation enriched Proteobacteria and Bacteroidota phyla, promoting functional guilds like Alcaligenes, Pseudomonas, and Paracoccus, which synergized with indigenous microbiota to enhance metabolic efficiency. This study highlights the potential of HN-AD consortia to overcome limitations of conventional nitrogen removal systems, offering a promising strategy for optimizing wastewater treatment processes. Full article
Show Figures

Figure 1

Back to TopTop