Biological Wastewater Treatment and Resource Recovery, 2nd Edition

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Biochemical Engineering".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 6310

Editors


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Department of Community Planning and Natural Resources, Lulea University of Technology, 971 87 Lulea, Sweden
Interests: acidogenic fermentation; anaerobic digestion; volatile Fatty acids; biohydrogen; bioammonium; biological wastewater treatment
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Guest Editor
Department of Chemical Engineering, Massachusetts Institute of Technology (MIT), Cambridge, CA, USA
Interests: bioenergy; biohydrogen; renewable chemicals; biopolymers; biorefinery
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Guest Editor
Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-gu, Gwangju, Republic of Korea
Interests: microbial electrochemical technologies; electro-fermentation; heavy metals; environmental biotechnology; bioenergy
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Special Issue Information

Dear Colleagues,

Biological wastewater treatment is an essential process in the purification and recycling of water resources that aims to remove various contaminants from wastewater using natural biological mechanisms. Wastewater possesses an abundant amount of nutrients that may lead to significant eutrophication if discharged into water bodies without any treatment. In the past, wastewater has been considered as a significant concern for both the environment and public health. However, with the development of sustainable technologies, it is now recognized as a valuable resource. Energy is required for effective biological treatment processes, and incomplete nitrification/denitrification processes can release greenhouse gases such as nitrous oxide, which is more potent than CO2. Wastewater treatment plants can treat wastewater by removing nitrogen (N), phosphorus (P), and organic pollutants. However, it is crucial to extract nutrients and valorize the wastewater, which may be possible by combining its treatment with resource recovery.

The effectiveness of biological wastewater treatment relies on the principles of biodegradation and microbial metabolism. Through a series of well-designed processes, the organic matter, nutrients, pathogens, and toxic substances present in wastewater can be efficiently degraded and removed. Oxygen availability, temperature, pH levels, retention time, and the availability of specific microorganisms are key factors that influence the performance and efficiency of biological treatment systems. The focus of the research in this area has been on learning about new communities of microorganisms; trying out new methods like biofilm reactors, activated sludge systems, and constructed wetlands; and looking into how advanced technologies like genetic engineering and nanotechnology can be used in the treatment process. There are many methods for the valorization of wastewater, such as anaerobic digestion (AD) and bioelectrogenesis. These processes harness the power of microorganisms, such as bacteria, fungi, and algae, to break down organic and inorganic pollutants and enable the extraction of bioenergy in the form of biogas (specifically biomethane, biohydrogen, and bioelectricity), which leads to the production of biobased products such as platform chemicals, volatile fatty acids, and bioammonium.

This Special Issue aims to address the advancements, challenges, and opportunities in the field of biological wastewater treatment. It welcomes interdisciplinary studies encompassing microbiology, engineering, chemistry, biotechnology, and environmental sciences. Additionally, topics such as (i) biotechnological methods to extract resources from wastewater and turn them into value-added products, (ii) the integration of processes for bioenergy and biochemical production, and (iii) nutrient recovery for the circular economy and environmental management will be considered in this SI. The goal is to contribute to the development of sustainable wastewater treatment technologies that minimize environmental impact and maximize resource recovery. By disseminating cutting-edge research, this Special Issue aims to foster collaboration and encourage the adoption of novel approaches in the field of biological wastewater treatment.

Dr. Omprakash Sarkar
Dr. Naresh Kumar Amradi
Dr. J. Shanthi Sravan
Guest Editor

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Keywords

  • wastewater treatment
  • nutrient recovery
  • bioenergy generation
  • biobased products
  • anaerobic digestion
  • volatile fatty acids
  • bioammonium
  • bioelectricity

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Published Papers (2 papers)

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Research

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29 pages, 3215 KB  
Article
Techno-Economic Assessment of Integrated Wastewater Technologies for Sustainable Treatment of Highly Loaded Landfill Leachate Using GPS-XTM
by Abdulmohsen Abdulkarim Mohammed Alkunaydiri, Nuhu Dalhat Mu’azu and Ahmad Hussaini Jagaba
Bioengineering 2026, 13(3), 359; https://doi.org/10.3390/bioengineering13030359 - 19 Mar 2026
Viewed by 1031
Abstract
Landfill leachate is considered one of the most recalcitrant wastewaters due to its high organic strength, elevated ammonia concentrations, and complex chemical composition. This study evaluates integrated technologies for treating highly loaded landfill leachate from the Wadi Al-Asla landfill, Jeddah Saudi Arabia, using [...] Read more.
Landfill leachate is considered one of the most recalcitrant wastewaters due to its high organic strength, elevated ammonia concentrations, and complex chemical composition. This study evaluates integrated technologies for treating highly loaded landfill leachate from the Wadi Al-Asla landfill, Jeddah Saudi Arabia, using GPS-XTM modeling combined with regulatory compliance and techno-economic assessment (TEA). The characterized mature leachate exhibited extremely high average concentrations of COD (17,050 mg L−1), BOD5 (10,058 mg L−1), ammonia-N (989 mg L−1), and total nitrogen (1223 mg L−1), indicating severe pollution levels requiring integrated treatment technologies. Five (5) different scenarios involving integrated biological, physicochemical, and membrane-based processes were modelled, simulated and evaluated against local discharge standards complaince. Conventional and municipality-proposed upgrade configurations achieved ~80–83% COD removal, producing effluent COD > 2900 mg L−1 and 1790–1801 mg L−1 BOD5, indicating persistent non-compliance for organic pollutants. Nitrogen removal improved substantially (93.7–95.7% ammonia-N and 91–93% total nitrogen removal), yet residual ammonia-N (44–63 mg L−1) and total nitrogen (92–108 mg L−1) remained above regulatory limits. Advanced hybrid systems achieved complete TSS removal and strong phosphorus control (TP ≤ 0.42 mg L−1), while three(3) compartmental aerobic–anoxic membrane bioreactor coupled with reverse osmosis (MBR + RO) achieved near-complete nitrogen removal and reduced 90% COD removal. The lifecyle economic assessment indicated OPEX ranging from USD 1.1 to 5.6 m−3 of treated leachate with the aerobic–anoxic MBR + RO configuration yieding footprint advantage, lower CAPEX and moderate OPEX By combining process modeling, regulatory compliance evaluation, and economic assessment, this study provides a practical screening framework for selecting sustainable treatment strategies for high-strength landfill leachate and wastewater matices. Full article
(This article belongs to the Special Issue Biological Wastewater Treatment and Resource Recovery, 2nd Edition)
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Review

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24 pages, 1403 KB  
Review
Environmental Impacts and Strategies for Bioremediation of Dye-Containing Wastewater
by Mukesh Kumar, Anshuman Mishra, Suresh Kumar Patel, Jyoti Kushwaha, Sunita Singh, Vinay Mishra, Deepak Singh, Vijay Singh, Balendu Shekher Giri, Reeta Rani Singhania and Dhananjay Singh
Bioengineering 2025, 12(10), 1043; https://doi.org/10.3390/bioengineering12101043 - 28 Sep 2025
Cited by 14 | Viewed by 4719
Abstract
Rapid industrialization, along with the development of textile and other associated industries, has led to the discharge of dyes, heavy metals, and other carcinogenic and environmentally harmful substances into water bodies. The volume of wastewater containing dyes is increasing day by day. Raised [...] Read more.
Rapid industrialization, along with the development of textile and other associated industries, has led to the discharge of dyes, heavy metals, and other carcinogenic and environmentally harmful substances into water bodies. The volume of wastewater containing dyes is increasing day by day. Raised levels of dyes, along with other contaminants, in wastewater are becoming a global concern, as these affect human health as well as aquatic flora and fauna. Bioremediation is one of the effective, sustainable, eco-friendly and cost-effective approaches for the treatment of wastewater containing dyes. This paper presents a state-of-the-art review of bioremediation techniques used for the removal of dyes from textile wastewater. The usage of various strains, e.g., bacteria, algae, yeast, enzymes, fungi, etc., is discussed in detail. Bioremediation of dyes using bioreactors and microbial fuel cells is also explored in this study. Full article
(This article belongs to the Special Issue Biological Wastewater Treatment and Resource Recovery, 2nd Edition)
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