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Bioengineering
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Stem Cell for Tissue Engineering
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Stem Cell for Tissue Engineering

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Regenerative Engineering".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 5235

Special Issue Editors

Dr. Andrea Dell’Amore

E-Mail Website
Guest Editor
Thoracic Surgery Division, Department of Cardiac, Thoracic, Vascular Sciences and Public Health, Padova University Hospital, 35128 Padova, Italy
Interests: heart and lung transplantation; pulmonary artery hypertension; pulmonary thromboendoarterctomy; ECMO; cardiac assistance devices; tracheal surgery; robotic surgery; minimally invasive cardiothoracic surgery
Dr. Marco Mammana

E-Mail Website
Guest Editor
Thoracic Surgery Division, Department of Cardiac, Thoracic, Vascular Sciences and Public Health, University of Padova, 35128 Padova, Italy
Interests: lung transplantation; marginal donors; thymic tumor; vascular involvement; surgery; tumor recurrence

Special Issue Information

Dear Colleagues,

Research on the application of stem cells in clinical medicine is now a reality in many countries and the results of therapies based on the use of stem cells are bringing incredible results. In particular, the use of stem cells in tissue engineering represents new innovations in biomedical research, including regenerative medicine, organ transplants, and the treatment of genetic diseases.

This Special Issue aims to show all the possible applications of these techniques in the different fields of medicine and reports the progress and practical clinical applications of the use of stem cells in clinical practice. Particular attention will be paid to the search for the regeneration of organs for the purpose of transplantation, and the creation of tissues that can be used in the repair and regeneration of irreparably damaged tissues, without regeneration. Research on the rehabilitation of biological scaffolds or decellularized tissues for the creation of organs, such as the heart, which can be considered a valid alternative to artificial hearts or particularly complex structures to replace such as the trachea, heart valves, or osseocartilaginous tissues, is of particular interest.

Dr. Andrea Dell’Amore
Dr. Marco Mammana
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • stem cells
  • organ trasplantation
  • bioengineering
  • biological scaffolds
  • organ regeneration
  • decellularized matrix
  • ex vivo model
  • mesenchymal cells
  • cellular colture
  • tissue regeneration

Published Papers (3 papers)

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Research

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20 pages, 5549 KiB  
Article
Effects of Oral Cavity Stem Cell Sources and Serum-Free Cell Culture on Hydrogel Encapsulation of Mesenchymal Stem Cells for Bone Regeneration: An In Vitro Investigation
by Premjit Arpornmaeklong, Supakorn Boonyuen, Komsan Apinyauppatham and Prisana Pripatnanont
Bioengineering 2024, 11(1), 59; https://doi.org/10.3390/bioengineering11010059 - 8 Jan 2024
Viewed by 1275
Abstract
Introduction: To develop a stem cell delivery model and improve the safety of stem cell transplantation for bone regeneration, this study aimed to determine the effects of stem cell sources, serum-free cell culture, and hydrogel cell encapsulation on the growth and osteogenic differentiation [...] Read more.
Introduction: To develop a stem cell delivery model and improve the safety of stem cell transplantation for bone regeneration, this study aimed to determine the effects of stem cell sources, serum-free cell culture, and hydrogel cell encapsulation on the growth and osteogenic differentiation of mesenchymal stem cells (MSCs) from the oral cavity. Methods: The study groups were categorized according to stem cell sources into buccal fat pad adipose (hBFP-ADSCs) (Groups 1, 4, and 7), periodontal ligament (hPDLSCs) (Groups 2, 5, and 8), and dental pulp-derived stem cells (hDPSCs) (Groups 3, 6, and 9). MSCs from each source were isolated and expanded in three types of sera: fetal bovine serum (FBS) (Groups 1–3), human serum (HS) (Groups 4–6), and synthetic serum (SS) (StemPro™ MSC SFM) (Groups 7–9) for monolayer (m) and hydrogel cell encapsulation cultures (e). Following this, the morphology, expression of MSC cell surface antigens, growth, and osteogenic differentiation potential of the MSCs, and the expression of adhesion molecules were analyzed and compared. Results: SS decreased variations in the morphology and expression levels of cell surface antigens of MSCs from three cell sources (Groups 7m–9m). The levels of osteoblastic differentiation of the hPDLSCs and hBFP-ADSCs were increased in SS (Groups 8m and 7m) and the cell encapsulation model (Groups 1e, 4e, 7e–9e), but the promoting effects of SS were decreased in a cell encapsulation model (Groups 7e–9e). The expression levels of the alpha v beta 3 (ITG-αVβ3) and beta 1 (ITG-β1) integrins in the encapsulated cells in FBS (Group 1e) were higher than those in the SS (Group 7e). Conclusions: Human PDLSCs and BFP-ADSCs were the optimum stem cell source for stem cell encapsulation by using nanohydroxyapatite–calcium carbonate microcapsule–chitosan/collagen hydrogel in serum-free conditions. Full article
(This article belongs to the Special Issue Stem Cell for Tissue Engineering)
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13 pages, 5265 KiB  
Article
Erectile Dysfunction Treatment Using Stem Cell Delivery Patch in a Cavernous Nerve Injury Rat Model
by Hyong Woo Moon, In Gul Kim, Mee Young Kim, Ae Ryang Jung, Kwideok Park and Ji Youl Lee
Bioengineering 2023, 10(6), 635; https://doi.org/10.3390/bioengineering10060635 - 24 May 2023
Cited by 1 | Viewed by 2290
Abstract
Erectile dysfunction (ED) is a common and feared complication of radical prostatectomy (RP) for prostate cancer. Recently, tissue engineering for post-prostatectomy ED has been attempted in which controlled interactions between cells, growth factors, and the extracellular matrix (ECM) are important for the structural [...] Read more.
Erectile dysfunction (ED) is a common and feared complication of radical prostatectomy (RP) for prostate cancer. Recently, tissue engineering for post-prostatectomy ED has been attempted in which controlled interactions between cells, growth factors, and the extracellular matrix (ECM) are important for the structural integrity if nerve regeneration. In this study, we evaluated the effects of a biomechanical ECM patch on the morphology and behavior of human bone marrow-derived mesenchymal stem cells (hBMSCs) in a bilateral cavernous nerve injury (BCNI) rat model. The ECM patch, made of decellularized human fibroblast-derived ECM (hFDM) and a biocompatible polyvinyl alcohol (PVA) hydrogel, was tested with human bone marrow-derived mesenchymal stem cells (hBMSCs) on a bilateral cavernous nerve injury (BCNI) rat model. In vitro analysis showed that the hFDM/PVA + hBMSCs patches significantly increased neural development markers. In vivo experiments demonstrated that the rats treated with the hFDM/PVA patch had higher ICP/MAP ratios, higher ratios of smooth muscle to collagen, increased nNOS content, higher levels of eNOS protein expression, and higher cGMP levels compared to the BCNI group. These results indicate that the hFDM/PVA patch is effective in promoting angiogenesis, smooth muscle regeneration, and nitrergic nerve regeneration, which could contribute to improved erectile function in post-prostatectomy ED. Full article
(This article belongs to the Special Issue Stem Cell for Tissue Engineering)
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Review

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16 pages, 1552 KiB  
Review
Tracheal Tissue Engineering: Principles and State of the Art
by Marco Mammana, Alessandro Bonis, Vincenzo Verzeletti, Andrea Dell’Amore and Federico Rea
Bioengineering 2024, 11(2), 198; https://doi.org/10.3390/bioengineering11020198 - 19 Feb 2024
Viewed by 1296
Abstract
Patients affected by long-segment tracheal defects or stenoses represent an unsolved surgical issue, since they cannot be treated with the conventional surgery of tracheal resection and consequent anastomosis. Hence, different strategies for tracheal replacement have been proposed (synthetic materials, aortic allografts, transplantation, autologous [...] Read more.
Patients affected by long-segment tracheal defects or stenoses represent an unsolved surgical issue, since they cannot be treated with the conventional surgery of tracheal resection and consequent anastomosis. Hence, different strategies for tracheal replacement have been proposed (synthetic materials, aortic allografts, transplantation, autologous tissue composites, and tissue engineering), each with advantages and drawbacks. Tracheal tissue engineering, on the other hand, aims at recreating a fully functional tracheal substitute, without the need for the patient to receive lifelong immunosuppression or endotracheal stents. Tissue engineering approaches involve the use of a scaffold, stem cells, and humoral signals. This paper reviews the main aspects of tracheal TE, starting from the choice of the scaffold to the type of stem cells that can be used to seed the scaffold, the methods for their culture and expansion, the issue of graft revascularization at the moment of in vivo implantation, and experimental models of tracheal research. Moreover, a critical insight on the state of the art of tracheal tissue engineering is also presented. Full article
(This article belongs to the Special Issue Stem Cell for Tissue Engineering)
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