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Plant Biotechnology and Application

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Agricultural Science and Technology".

Deadline for manuscript submissions: 10 March 2027 | Viewed by 1041

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Guest Editor
Faculty of Biotechnology, Collegium Medicum, University of Rzeszów, Pigonia 1 St., 35-310 Rzeszów, Poland
Interests: improving agricultural crop production technology; impact of crop technology on plant quality and food safety; exogenous use of phytoprotectants; biochemical and physiological responses of plants to abiotic stresses in crop production
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant biotechnology is playing an increasingly pivotal role in shaping modern agriculture and environmental sciences. The rapid development of molecular biology, genomics, metabolomics, and bioinformatics has provided deeper insights into the mechanisms regulating plant metabolism, stress responses, and adaptive capacity. The integration of these disciplines enables not only the exploration of the fundamental processes underlying plant function but also the design of targeted biotechnological interventions aimed at improving yield, quality, and resilience to environmental stressors.

This Special Issue, “Plant Biotechnology and Application”, aims to present the latest achievements in both fundamental and applied plant biotechnology. It focuses on innovations that bridge experimental science with field and industrial applications, creating a link between molecular discoveries and real-world challenges in sustainable agriculture.

The scope of this issue includes, but is not limited to, the following:

  • Molecular, physiological, and biochemical mechanisms of plant responses to abiotic and biotic stress;
  • Genetic engineering, CRISPR/Cas-based genome editing, and advanced strategies in molecular breeding;
  • Metabolic engineering of primary and secondary metabolites to enhance the nutritional, functional, and industrial value of crops;
  • Plant–microbe interactions and microbial biotechnology for sustainable crop production;
  • Omics-based and bioinformatics approaches for gene discovery and systems biology;
  • Biotechnological strategies applied in phytoremediation, bioenergy production, and circular bioeconomy.

This Special Issue welcomes original research articles, reviews, and short communications presenting innovative biotechnological solutions and their practical implementation in plant science, agriculture, environmental protection, and industry. It seeks to provide an interdisciplinary forum for the exchange of knowledge and experience, promoting the development of modern, sustainable biotechnological innovations for the future of plant production.

Dr. Dagmara Migut
Guest Editor

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant biotechnology
  • genetic engineering
  • stress physiology
  • metabolic engineering
  • sustainable agriculture

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Published Papers (1 paper)

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Research

10 pages, 3876 KB  
Article
Simple Improvement of In Vitro Shoot Elongation and Rooting of Sida cordifolia L. from Nodal Segments
by Juraj Habdák, Šarlota Kaňuková and Ján Kraic
Appl. Sci. 2026, 16(7), 3423; https://doi.org/10.3390/app16073423 - 1 Apr 2026
Cited by 1 | Viewed by 624
Abstract
Efficient in vitro propagation of Sida cordifolia L. from nodal explants is often limited by poor shoot and root elongation. This study aimed to improve elongation of shoots and roots from two-node explants through simple modifications of the Murashige and Skoog (MS) culture [...] Read more.
Efficient in vitro propagation of Sida cordifolia L. from nodal explants is often limited by poor shoot and root elongation. This study aimed to improve elongation of shoots and roots from two-node explants through simple modifications of the Murashige and Skoog (MS) culture medium. Explants were cultured on full- and half-strength MS media. Half-strength MS (1/2MS) significantly increased shoot (3.9-fold) and root (4.2-fold) elongation compared to full-strength MS. Therefore, subsequent experiments were conducted using 1/2MS. Further modifications included supplementation of 1/2MS medium with KH2PO4, plant growth regulators (TDZ, BAP, 2iP, GA3), and pretreatment of nodal segments with Norit buffer before cultivation. KH2PO4 did not further affect shoot elongation but significantly enhanced root elongation, with the greatest effect at 170 mg L−1. Pretreatment of nodal segments in Norit buffer had almost the same effect as KH2PO4, both on shoots and roots. In contrast, cytokinins and GA3 inhibited both shoot and root elongation compared to hormone-free 1/2MS medium. The results indicate that 1/2MS medium alone is most suitable for improving shoot and root elongation in vitro in S. cordifolia. Supplementation of the 1/2MS medium with 170 mg L−1 KH2PO4 further enhances root elongation. The use of cytokinins and GA3 should be avoided. Full article
(This article belongs to the Special Issue Plant Biotechnology and Application)
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