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Hydrogen Peroxide Signaling in Physiology and Pathology

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A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "ROS, RNS and RSS".

Deadline for manuscript submissions: closed (20 October 2022) | Viewed by 11865

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Special Issue Editors

Dr. Christine Rampon

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Guest Editor

Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, PSL Research University, 75231 Paris, France
Interests: redox signaling; regeneration; development; morphogenesis

Prof. Dr. Sophie Vriz

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Guest Editor

Special Issue Information

Dear Colleagues,

Reactive oxygen species (ROS) were originally described as toxic by-products of aerobic cellular energy metabolism associated with the development of several diseases, such as cancer, neurodegenerative diseases, and diabetes. In these situations, the accumulation of ROS in cells, referred to as oxidative stress, is a toxic event that damages a number of biomolecules. However, recent findings have shown that ROS can also contribute to bona fide physiological processes, leading to a new paradigm in reversible posttranslational modifications involved in signal transduction, defined as oxidative eustress. Amongst ROS, hydrogen peroxide (H₂O₂) best fits the properties of a signalling molecule and is recognized as the major ROS in the oxidative regulation of physiological activity. H₂O₂ is mainly produced by NAPDH oxidases and the mitochondrial electron transport chain. This generation is controlled by growth factors, chemokines and physical stress, among other factors. A Special Issue of Antioxidants will be devoted to these topics.

We invite you to submit your latest research findings or a review article to this Special Issue. We welcome submissions concerning the analysis of H₂O₂ dynamics from the subcellular to organism level, as well as the identification of molecular targets or H₂O₂-regulated processes in animals or plants in physiological or pathological contexts. We believe that this Special Issue will help to highlight the most recent advances in all the aspects of H₂O₂ signalling.

We look forward to your contribution.

Dr. Christine Rampon
Prof. Dr. Sophie Vriz
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antioxidants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

Hydrogen peroxide
Redox signaling
Physiology
Pathology
Redox signaling
Oxidative eustress
Oxidative distress
H₂O₂ dynamics
H₂O₂ patterns

Published Papers (6 papers)

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Editorial

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2 pages, 189 KiB

Open AccessEditorial

Hydrogen Peroxide Signaling in Physiology and Pathology

by Christine Rampon and Sophie Vriz

Antioxidants 2023, 12(3), 661; https://doi.org/10.3390/antiox12030661 - 07 Mar 2023

Cited by 1 | Viewed by 906

Abstract

(This article belongs to the Special Issue Hydrogen Peroxide Signaling in Physiology and Pathology)

Research

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14 pages, 2806 KiB

Open AccessArticle

Oxidative Stress-Induced Overactivation of Frog Eggs Triggers Calcium-Dependent Non-Apoptotic Cell Death

by Alexander A. Tokmakov, Yudai Morichika, Ryuga Teranishi and Ken-Ichi Sato

Antioxidants 2022, 11(12), 2433; https://doi.org/10.3390/antiox11122433 - 09 Dec 2022

Cited by 1 | Viewed by 1261

Abstract

Excessive activation of frog eggs (overactivation) is a pathological process that renders eggs unfertilizable. Its physiological inducers are unknown. Previously, oxidative stress was shown to cause time- and dose-dependent overactivation of Xenopus laevis frog eggs. Here, we demonstrate that the oxidative stress-induced egg overactivation is a calcium-dependent phenomenon which can be attenuated in the presence of the selective calcium chelator BAPTA. Degradation of cyclin B2, which is known to be initiated by calcium transient in fertilized or parthenogenetically activated eggs, can also be observed in the overactivated eggs. Decline in mitochondrial membrane potential, ATP depletion and termination of protein synthesis manifest in the eggs within one hour of triggering overactivation. These intracellular events occur in the absence of caspase activation. Furthermore, plasma membrane integrity is compromised in the overactivated eggs, as evidenced by ATP leakage and egg swelling. In sum, our data demonstrate that oxidative stress-induced overactivation of frog eggs causes fast and dramatic disruption of cellular homeostasis, resulting in robust and expedited cell death by a calcium-dependent non-apoptotic mechanism. Full article

(This article belongs to the Special Issue Hydrogen Peroxide Signaling in Physiology and Pathology)

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12 pages, 2495 KiB

Open AccessArticle

H₂O₂ and Ca²⁺ Signaling Crosstalk Counteracts ABA to Induce Seed Germination

by Mengjie Cheng, Yanliang Guo, Qing Liu, Sanwa Nan, Yuxing Xue, Chunhua Wei, Yong Zhang, Feishi Luan, Xian Zhang and Hao Li

Antioxidants 2022, 11(8), 1594; https://doi.org/10.3390/antiox11081594 - 17 Aug 2022

Cited by 4 | Viewed by 2090

Abstract

Seed germination is a critical stage and the first step in the plant’s life cycle. H₂O₂ and Ca²⁺ act as important signal molecules in regulating plant growth and development and in providing defense against numerous stresses; however, their crosstalk in modulating seed germination remains largely unaddressed. In the current study, we report that H₂O₂ and Ca²⁺ counteracted abscisic acid (ABA) to induce seed germination in melon and Arabidopsis by modulating ABA and gibberellic acid (GA₃) balance. H₂O₂ treatment induced a Ca²⁺ influx in melon seeds accompanied by the upregulation of cyclic nucleotide-gated ion channel(CNGC) 20, which encodes a plasma membrane Ca²⁺-permeable channel. However, the inhibition of cytoplasmic free Ca²⁺ elevation in the melon seeds and Arabidopsis mutant atcngc20 compromised H₂O₂-induced germination under ABA stress. CaCl₂ induced H₂O₂ accumulation accompanied by the upregulation of respiratory burst oxidase homologue(RBOH) D and RBOHF in melon seeds with ABA pretreatment. However, inhibition of H₂O₂ accumulation in the melon seeds and Arabidopsis mutant atrbohd and atrbohf abolished CaCl₂-induced germination under ABA stress. The current study reveals a novel mechanism in which H₂O₂ and Ca²⁺ signaling crosstalk offsets ABA to induce seed germination. H₂O₂ induces Ca²⁺ influx, which in turn increases H₂O₂ accumulation, thus forming a reciprocal positive-regulatory loop to maintain a balance between ABA and GA₃ and promote seed germination under ABA stress. Full article

(This article belongs to the Special Issue Hydrogen Peroxide Signaling in Physiology and Pathology)

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10 pages, 2469 KiB

Open AccessArticle

In Vivo Assessment of Antioxidant Potential of Human Milk Treated by Holder Pasteurization or High Hydrostatic Pressure Processing: A Preliminary Study on Intestinal and Hepatic Markers in Adult Mice

by Eve Wemelle, Lucie Marousez, Jean Lesage, Marie De Lamballerie, Claude Knauf and Lionel Carneiro

Antioxidants 2022, 11(6), 1091; https://doi.org/10.3390/antiox11061091 - 31 May 2022

Cited by 3 | Viewed by 1608

Abstract

Preterm infants are highly susceptible to oxidative stress due to an imbalance between endogenous oxidant and antioxidant systems. In addition, these newborns are frequently fed with donor milk (DM) treated by Holder pasteurization (HoP) at 62.5 °C for 30 min, which is known to alter numerous heat-sensitive factors, including some antioxidants. High hydrostatic pressure (HHP) processing was recently proposed as an innovative method for the treatment of DM. The present study aimed to measure the redox balance of HoP- and HHP-DM and to study, in vivo, the effects of HoP- and HHP-DM on the gut and liver. H₂O₂, vitamin A and vitamin E (α- and γ-tocopherols) concentrations, as well as the total antioxidant capacity (TAC), were measured in raw-, HoP- and HHP-DM. The gene expression level of antioxidant systems and inflammatory response were quantified in the ileum and liver of adult mice after 7 days of oral administration of HoP- or HHP-DM. HoP reduced the γ-tocopherol level, whereas HHP treatment preserved all vitamins close to the raw milk level. The milk H₂O₂ content was reduced by HHP but not by HoP. The total antioxidant capacity of DM was reduced after HHP processing measured by PAOT-Liquid^® technology but was unaffected after measurement by ORAC assay. In mice, HHP-DM administration induced a stimulation of antioxidant defenses and reduced some inflammatory markers in both the ileum and liver compared to HoP-DM treatment. Our preliminary study suggests that the HHP processing of DM may better protect preterm infants from gut and liver pathologies compared to HoP, which is currently used in most human milk banks. Full article

(This article belongs to the Special Issue Hydrogen Peroxide Signaling in Physiology and Pathology)

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17 pages, 3952 KiB

Open AccessArticle

Reciprocal Regulation of Shh Trafficking and H₂O₂ Levels via a Noncanonical BOC-Rac1 Pathway

by Marion Thauvin, Irène Amblard, Christine Rampon, Aurélien Mourton, Isabelle Queguiner, Chenge Li, Arnaud Gautier, Alain Joliot, Michel Volovitch and Sophie Vriz

Antioxidants 2022, 11(4), 718; https://doi.org/10.3390/antiox11040718 - 05 Apr 2022

Cited by 4 | Viewed by 2271

Abstract

Among molecules that bridge environment, cell metabolism, and cell signaling, hydrogen peroxide (H₂O₂) recently appeared as an emerging but central player. Its level depends on cell metabolism and environment and was recently shown to play key roles during embryogenesis, contrasting with its long-established role in disease progression. We decided to explore whether the secreted morphogen Sonic hedgehog (Shh), known to be essential in a variety of biological processes ranging from embryonic development to adult tissue homeostasis and cancers, was part of these interactions. Here, we report that H₂O₂ levels control key steps of Shh delivery in cell culture: increased levels reduce primary secretion, stimulate endocytosis and accelerate delivery to recipient cells; in addition, physiological in vivo modulation of H₂O₂ levels changes Shh distribution and tissue patterning. Moreover, a feedback loop exists in which Shh trafficking controls H₂O₂ synthesis via a non-canonical BOC-Rac1 pathway, leading to cytoneme growth. Our findings reveal that Shh directly impacts its own distribution, thus providing a molecular explanation for the robustness of morphogenesis to both environmental insults and individual variability. Full article

(This article belongs to the Special Issue Hydrogen Peroxide Signaling in Physiology and Pathology)

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20 pages, 3050 KiB

Open AccessArticle

Transcriptomic Analysis of E. coli after Exposure to a Sublethal Concentration of Hydrogen Peroxide Revealed a Coordinated Up-Regulation of the Cysteine Biosynthesis Pathway

by Myriam Roth, Vincent Jaquet, Sylvain Lemeille, Eve-Julie Bonetti, Yves Cambet, Patrice François and Karl-Heinz Krause

Antioxidants 2022, 11(4), 655; https://doi.org/10.3390/antiox11040655 - 28 Mar 2022

Cited by 10 | Viewed by 2865

Abstract

Hydrogen peroxide (H₂O₂) is a key defense component of host-microbe interaction. However, H₂O₂ concentrations generated by immune cells or epithelia are usually insufficient for bacterial killing and rather modulate bacterial responses. Here, we investigated the impact of sublethal H₂O₂ concentration on gene expression of E. coli BW25113 after 10 and 60 min of exposure. RNA-seq analysis revealed that approximately 12% of bacterial genes were strongly dysregulated 10 min following exposure to 2.5 mM H₂O₂. H₂O₂ exposure led to the activation of a specific antioxidant response and a general stress response. The latter was characterized by a transient down-regulation of genes involved in general metabolism, such as nucleic acid biosynthesis and translation, with a striking and coordinated down-regulation of genes involved in ribosome formation, and a sustained up-regulation of the SOS response. We confirmed the rapid transient and specific response mediated by the transcription factor OxyR leading to up-regulation of antioxidant systems, including the catalase-encoding gene (katG), that rapidly degrade extracellular H₂O₂ and promote bacterial survival. We documented a strong and transient up-regulation of genes involved in sulfur metabolism and cysteine biosynthesis, which are under the control of the transcription factor CysB. This strong specific transcriptional response to H₂O₂ exposure had no apparent impact on bacterial survival, but possibly replenishes the stores of oxidized cysteine and glutathione. In summary, our results demonstrate that different stress response mechanisms are activated by H₂O₂ exposure and highlight the cysteine synthesis as an antioxidant response in E. coli. Full article

(This article belongs to the Special Issue Hydrogen Peroxide Signaling in Physiology and Pathology)

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