Phenolic Profiling and Antioxidant Capacity of Natural Products

A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Natural and Synthetic Antioxidants".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 519

Special Issue Editor


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Guest Editor
Department of Physiology and Pharmacology, Physiology Unit, Veterinary Faculty, University of Zaragoza, 50009 Zaragoza, Spain
Interests: intestinal absorption of nutrients; sepsis state (LPS, cytokines, and inflammation); plant extracts and olive oil (squalene) for therapeutic purposes; encapsulation of plant extracts to improve their bioavailability; antioxidant properties of plant extracts; antioxidant markers; studies (modulators, pathways, and targets) of apoptosis, necroptosis, and autophagy; cell culture; cancer and cardiovascular diseases; gold complexes; colorectal cancer
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Special Issue Information

Dear Colleagues,

Since ancient times, natural products have been considered an important source of bioactive compounds with therapeutic potential; therefore, topics related to food and nutrition are receiving significant interest in biomedical research.

Among these bioactive compounds are those with phenolic profiles that are present in different plant species; these represent the largest group of non-energy compounds in plant-based foods. Various epidemiological studies have shown correlations between high consumption of cereals, fruits, and vegetables and a lower risk of diseases associated with oxidative stress, such as cancer, inflammation, osteoarthritis, cardiovascular disease, and diabetes. These protective effects could be related to the antioxidant properties of phenolic compounds. Therefore, some of these phytochemicals have been used for medicinal purposes as dietary supplements, traditional herbal medicinal products, and even as isolated drugs. The antioxidant effects of phenolic compounds can be exerted directly by scavenging free radicals or indirectly by modulating gene expression and the activities of enzymes involved in redox homeostasis.

Prof. Dr. María Jesús Rodríguez-Yoldi
Guest Editor

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Keywords

  • plant extracts
  • antioxidant capacity
  • phenolic profiling
  • stress oxidative diseases

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Published Papers (1 paper)

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Research

21 pages, 2406 KB  
Article
Lactarius deliciosus Extract from Green Microwave-Assisted Eutectic Solvent Extraction as a Therapeutic Candidate Against Colon Cancer
by Seyed Hesamoddin Bidooki, Beatriz Rodríguez-Martínez, Javier Quero, Luis Vicente Herrera-Marcos, Mónica Paesa, Marina Delgado-Machuca, Oscar F. Beas-Guzmán, Jesús Osada, Pedro Ferreira-Santos and María Jesús Rodríguez-Yoldi
Antioxidants 2025, 14(12), 1452; https://doi.org/10.3390/antiox14121452 - 2 Dec 2025
Viewed by 405
Abstract
Lactarius deliciosus is a widely distributed edible mushroom valued as a functional food due to its rich content of nutrients, phenolic compounds, and flavonoids, which contribute to its strong antioxidant and antimicrobial properties. The present study aimed to optimize a green microwave-assisted extraction [...] Read more.
Lactarius deliciosus is a widely distributed edible mushroom valued as a functional food due to its rich content of nutrients, phenolic compounds, and flavonoids, which contribute to its strong antioxidant and antimicrobial properties. The present study aimed to optimize a green microwave-assisted extraction method for maximal recovery of bioactive phenolic compounds from Lactarius deliciosus extract (LDE) and to evaluate its antioxidant, antiproliferative, antimetastatic, and anti-inflammatory effects on human colon carcinoma (Caco-2) cells. The study demonstrated that solvent polarity and composition critically influence the recovery of antioxidant biomolecules, identifying water and NaDES 1 (glycerol/glycine/water) as the most efficient and sustainable solvents for microwave-assisted extraction at 225 °C. The LDE showed high levels of phenolic compounds—particularly 4-hydroxybenzoic and vanillic acids—indicating potent antioxidant potential and possible anticancer efficacy. The results revealed that the LDE significantly reduced colony formation and cell adhesion in a dose-dependent manner, leading to nearly complete inhibition of clonogenic survival at the IC50 concentration and a marked increase in cell death among non-adherent colon cancer cells. In addition, LDE inhibited the proliferation of Caco-2 cells by inducing G0/G1 cell cycle arrest and apoptosis, associated with altered mitochondrial potential and increased caspase-3 activity. The LDE modified the redox balance of the cell by decreasing the ROS levels and exerts anti-inflammatory effects through significant downregulation of NOS2 expression, without adversely affecting the intestinal barrier. The study concludes that LDE bioactive compounds show strong promise as anticancer and functional ingredients, demonstrating antioxidant, antiproliferative, antimetastatic, and anti-inflammatory effects. Full article
(This article belongs to the Special Issue Phenolic Profiling and Antioxidant Capacity of Natural Products)
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