One Health and Public Health Implications of Sterilization-Based Management in Free-Roaming Dogs and Cats

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Companion Animals".

Deadline for manuscript submissions: 10 July 2026 | Viewed by 965

Special Issue Editors


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Guest Editor
Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Paseo Blas Cabrera “Físico” s/n, 35016 Las Palmas de Gran Canaria, Spain
Interests: stray animal; trap–neuter–return; free-roaming cat management

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Guest Editor
Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran Canaria, Paseo Blas Cabrera “Físico” s/n, 35016 Las Palmas de Gran Canaria, Spain
Interests: toxicology; risk assessment; environmental health; One Health
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Special Issue Information

Dear Colleagues,

This Special Issue invites research and reviews on the sterilization-centered management of free-roaming dogs and cats (e.g., TNR/CNR) within a One Health and public-health framework. We welcome contributions that quantify population-level outcomes, animal welfare and sentinel health indicators, and public-health interfaces (zoonoses, AMR, environmental health).

Potential topics include the following:

  • Program coverage, continuity, and spatial design; evaluation frameworks linking effort to measurable outcomes (incidence, densities, welfare metrics);
  • Epidemiology at the human–animal–environment interface: zoonotic risks, AMR sentinels, and community health indicators;
  • Welfare assessment pre/post-intervention, perioperative safety, field anesthesia/analgesia, and pain/stress biomarkers;
  • Implementation science and governance: regulatory coherence, municipal practice, stakeholder engagement, and cost-effectiveness;
  • Methods and measurement: modelling, GIS, monitoring dashboards, molecular diagnostics, and data standards.

We especially encourage interdisciplinary research and policy-relevant studies from urban, peri-urban, rural, and insular settings. Purely lethal control studies without a One Health/public-health analytical component fall outside the scope of this Special Issue.

We look forward to receiving your contributions.

Dr. María del Mar Travieso-Aja
Prof. Dr. Octavio Pérez Luzardo
Guest Editors

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Keywords

  • One Health
  • one welfare
  • dogs
  • cats
  • human–animal interactions
  • free-roaming animal-related population management

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Published Papers (1 paper)

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Research

23 pages, 2002 KB  
Article
Low Zoonotic Pathogen Burden in Free-Roaming Cats Revealed by 18S rRNA Metabarcoding: A Baseline Study from an Insular Natura 2000 Site in Spain
by María del Mar Travieso-Aja, Luis Alberto Henríquez-Hernández, Elisa Hernández-Álvarez, Javier Quinteiro-Vázquez, Nieves E. González-Henríquez, Martina Cecchetti and Octavio P. Luzardo
Animals 2026, 16(3), 431; https://doi.org/10.3390/ani16030431 - 29 Jan 2026
Viewed by 679
Abstract
Free-roaming cats may contribute to zoonotic risk via parasites and other eukaryotic taxa, yet surveillance in protected island settings is limited and conventional coprology can miss low-intensity or degraded signals. We conducted a cross-sectional 18S rRNA metabarcoding survey to establish a baseline profile [...] Read more.
Free-roaming cats may contribute to zoonotic risk via parasites and other eukaryotic taxa, yet surveillance in protected island settings is limited and conventional coprology can miss low-intensity or degraded signals. We conducted a cross-sectional 18S rRNA metabarcoding survey to establish a baseline profile of potentially pathogenic eukaryotes in community cats from La Graciosa (Natura 2000, Canary Islands, Spain) prior to large-scale antiparasitic interventions. We analysed 152 faecal samples, including fresh samples collected during a high-throughput TNR campaign (n = 37) and dry environmental deposits (n = 115). Host amplification was reduced using a feline 18S blocking primer; libraries were sequenced with Oxford Nanopore technology; and taxonomy was assigned using SILVA-based classifiers with downstream filtering for veterinary/zoonotic relevance. After quality control, 72 eukaryotic taxa were retained and DNA from at least 24 potentially pathogenic taxa was detected. Dipylidium caninum was most frequent (74.3%; 113/152), and opportunistic fungi/yeasts were common (e.g., Pichia kudriavzevii 42.4%, Diutina catenulata 31.5%). Zoonotic protozoa showed low-to-moderate detection frequency (Acanthamoeba castellanii 13.3%, Toxoplasma gondii 7.9%, Balamuthia mandrillaris 4.6%). Overall richness did not differ between fresh and dry samples (p > 0.05), but fresh samples contained higher richness of potentially pathogenic taxa (p < 0.01). Full article
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