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19 pages, 1900 KiB

Open AccessArticle

Effect of Intramuscularly Administered Oxytetracycline or Enrofloxacin on Vancomycin-Resistant Enterococci, Extended Spectrum Beta-Lactamase- and Carbapenemase-Producing Enterobacteriaceae in Pigs

by Elena González-Fandos, Alba Martínez-Laorden, Ana Abad-Fau, Eloisa Sevilla, Rosa Bolea, María Jesús Serrano, Olga Mitjana, Cristina Bonastre, Alicia Laborda, María Victoria Falceto and Rafael Pagán

Animals 2022, 12(5), 622; https://doi.org/10.3390/ani12050622 - 1 Mar 2022

Cited by 5 | Viewed by 4556

Abstract

Nowadays, there is a great concern about the prevalence of multidrug resistant Enterococcus spp. and Enterobacteriaceae in food-producing animals. The aim of this work was to evaluate the effect of oxytetracycline or enrofloxacin treatment on vancomycin-resistant enterococci (VRE), extended spectrum β-lactamase (ESBL) and [...] Read more.

Nowadays, there is a great concern about the prevalence of multidrug resistant Enterococcus spp. and Enterobacteriaceae in food-producing animals. The aim of this work was to evaluate the effect of oxytetracycline or enrofloxacin treatment on vancomycin-resistant enterococci (VRE), extended spectrum β-lactamase (ESBL) and carbapenemase-producing Enterobacteriaceae in pigs. A total of 26 piglets were received and distributed in three groups. Group 1 was treated with enrofloxacin (N = 12), group 2 with oxytetracycline (N = 10) and group 3 did not receive any treatment (control group) (N = 4). A higher number of vancomycin-resistant E. faecium were recovered compared to E. faecalis. In the pigs treated with enrofloxacin, vancomycin resistant E. faecium was found in a higher percentage of animals than in the control group. ESBL-producing E. coli was not detected in rectal samples from control animals. However, it was detected in 17–20% of animals treated with oxytetracycline on days 6 to 17 and in 17–50% of the animals treated with enrofloxacin. Carbapenemase-producing E. coli was isolated in animals treated with oxytetracycline, but not in animals treated with enrofloxacin or in the control group. This study highlights that the use of oxytetracycline or enrofloxacin in food-producing animals could select ESBL and carbapenemase-producing E. coli. Further studies shall be needed to validate the results obtained, considering a more robust and extended experimental design. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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16 pages, 1714 KiB

Open AccessArticle

Diversity of Non-O157 Shiga Toxin-Producing Escherichia coli Isolated from Cattle from Central and Southern Chile

by Leonela Díaz, Sebastian Gutierrez, Andrea I Moreno-Switt, Luis Pablo Hervé, Christopher Hamilton-West, Nora Lía Padola, Paola Navarrete, Angélica Reyes-Jara, Jianghong Meng, Narjol González-Escalona and Magaly Toro

Animals 2021, 11(8), 2388; https://doi.org/10.3390/ani11082388 - 13 Aug 2021

Cited by 5 | Viewed by 4098

Abstract

Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and [...] Read more.

Cattle are the main reservoir of Shiga toxin-producing Escherichia coli (STEC), one of the world’s most important foodborne pathogens. The pathogen causes severe human diseases and outbreaks. This study aimed to identify and characterize non-O157 STEC isolated from cattle feces from central and southern Chile. We analyzed 446 cattle fecal samples and isolated non-O157 STEC from 12.6% (56/446); a total of 93 different isolates were recovered. Most isolates displayed β-glucuronidase activity (96.8%; 90/93) and fermented sorbitol (86.0%; 80/93), whereas only 39.8% (37/93) were resistant to tellurite. A subgroup of 30 representative non-O157 STEC isolates was selected for whole-genome sequencing and bioinformatics analysis. In silico analysis showed that they grouped into 16 different sequence types and 17 serotypes; the serotypes most frequently identified were O116:H21 and O168:H8 (13% each). A single isolate of serotype O26:H11 was recovered. One isolate was resistant to tetracycline and carried resistance genes tet(A) and tet(R); no other isolate displayed antimicrobial resistance or carried antimicrobial resistance genes. The intimin gene (eae) was identified in 13.3% (4/30) of the genomes and 90% (27/30) carried the stx2 gene. A phylogenetic reconstruction demonstrated that the isolates clustered based on serotypes, independent of geographical origin. These results indicate that cattle in Chile carry a wide diversity of STEC potentially pathogenic for humans based on the presence of critical virulence genes. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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17 pages, 3471 KiB

Open AccessArticle

Genomic Epidemiology of Shiga Toxin-Producing Escherichia coli Isolated from the Livestock-Food-Human Interface in South America

by Nicolás Galarce, Fernando Sánchez, Beatriz Escobar, Lisette Lapierre, Javiera Cornejo, Raúl Alegría-Morán, Víctor Neira, Víctor Martínez, Timothy Johnson, Danny Fuentes-Castillo, Elder Sano and Nilton Lincopan

Animals 2021, 11(7), 1845; https://doi.org/10.3390/ani11071845 - 22 Jun 2021

Cited by 17 | Viewed by 4410

Abstract

Shiga toxin-producing Escherichia coli (STEC) are zoonotic pathogens responsible for causing food-borne diseases in humans. While South America has the highest incidence of human STEC infections, information about the genomic characteristics of the circulating strains is scarce. The aim of this study was [...] Read more.

Shiga toxin-producing Escherichia coli (STEC) are zoonotic pathogens responsible for causing food-borne diseases in humans. While South America has the highest incidence of human STEC infections, information about the genomic characteristics of the circulating strains is scarce. The aim of this study was to analyze genomic data of STEC strains isolated in South America from cattle, beef, and humans; predicting the antibiotic resistome, serotypes, sequence types (STs), clonal complexes (CCs) and phylogenomic backgrounds. A total of 130 whole genome sequences of STEC strains were analyzed, where 39.2% were isolated from cattle, 36.9% from beef, and 23.8% from humans. The ST11 was the most predicted (20.8%) and included O-:H7 (10.8%) and O157:H7 (10%) serotypes. The successful expansion of non-O157 clones such as ST16/CC29-O111:H8 and ST21/CC29-O26:H11 is highlighted, suggesting multilateral trade and travel. Virulome analyses showed that the predominant stx subtype was stx2a (54.6%); most strains carried ehaA (96.2%), iha (91.5%) and lpfA (77.7%) genes. We present genomic data that can be used to support the surveillance of STEC strains circulating at the livestock-food-human interface in South America, in order to control the spread of critical clones “from farm to table”. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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15 pages, 1316 KiB

Open AccessArticle

Screening the Presence of Non-Typhoidal Salmonella in Different Animal Systems and the Assessment of Antimicrobial Resistance

by Dácil Rivera, Kasim Allel, Fernando Dueñas, Rodolfo Tardone, Paula Soza, Christopher Hamilton-West and Andrea I. Moreno-Switt

Animals 2021, 11(6), 1532; https://doi.org/10.3390/ani11061532 - 24 May 2021

Cited by 7 | Viewed by 5469

Abstract

Salmonella is a major bacterial foodborne pathogen that causes the majority of worldwide food-related outbreaks and hospitalizations. Salmonellosis outbreaks can be caused by multidrug-resistant (MDR) strains, emphasizing the importance of maintaining public health and safer food production. Nevertheless, the drivers of MDR Salmonella [...] Read more.

Salmonella is a major bacterial foodborne pathogen that causes the majority of worldwide food-related outbreaks and hospitalizations. Salmonellosis outbreaks can be caused by multidrug-resistant (MDR) strains, emphasizing the importance of maintaining public health and safer food production. Nevertheless, the drivers of MDR Salmonella serovars have remained poorly understood. In this study, we compare the resistance profiles of Salmonella strains isolated from 4047 samples from domestic and wild animals in Chile. A total of 106 Salmonella strains (2.61%) are isolated, and their serogroups are characterized and tested for susceptibility to 16 different antimicrobials. The association between antimicrobial resistance (AMR) and a subset of independent variables is evaluated using multivariate logistic models. Our results show that 47 antimicrobial-resistant strains were found (44.3% of the total strains). Of the 47, 28 correspond to single-drug resistance (SDR = 26.4%) and 19 are MDR (17.9%). S. Enteritidis is highly persistent in animal production systems; however, we report that serogroup D strains are 18 times less likely to be resistant to at least one antimicrobial agent than the most common serogroup (serogroup B). The antimicrobials presenting the greatest contributions to AMR are ampicillin, streptomycin and tetracycline. Additionally, equines and industrial swine are more likely to acquire Salmonella strains with AMR. This study reports antimicrobial-susceptible and resistant Salmonella in Chile by expanding the extant literature on the potential variables affecting antimicrobial-resistant Salmonella. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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12 pages, 1107 KiB

Open AccessArticle

Detection of Antimicrobial Residues in Poultry Litter: Monitoring a Risk through a Selective and Sensitive HPLC–MS/MS Method

by Karina Yévenes, Ekaterina Pokrant, Lina Trincado, Lisette Lapierre, Nicolás Galarce, Betty San Martín, Aldo Maddaleno, Héctor Hidalgo and Javiera Cornejo

Animals 2021, 11(5), 1399; https://doi.org/10.3390/ani11051399 - 14 May 2021

Cited by 16 | Viewed by 4199

Abstract

Tetracyclines, sulphonamides, and quinolones are families of antimicrobials (AMs) widely used in the poultry industry and can excrete up to 90% of AMs administrated, which accumulate in poultry litter. Worryingly, poultry litter is widely used as an agriculture fertilizer, contributing to the spread [...] Read more.

Tetracyclines, sulphonamides, and quinolones are families of antimicrobials (AMs) widely used in the poultry industry and can excrete up to 90% of AMs administrated, which accumulate in poultry litter. Worryingly, poultry litter is widely used as an agriculture fertilizer, contributing to the spread AMs residues in the environment. The aim of this research was to develop a method that could simultaneously identify and quantify three AMs families in poultry litter by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). Samples of AMs free poultry litter were used to validate the method according to 657/2002/EC and VICH GL49. Results indicate that limit of detection (LOD) ranged from 8.95 to 20.86 μg kg⁻¹, while limits of quantitation (LOQ) values were between 26.85 and 62.58 µg kg⁻¹ of tetracycline, 4-epi-tetracycline, oxytetracycline, 4-epi-oxytetracycline, enrofloxacin, ciprofloxacin, flumequine, sulfachloropyridazine, and sulfadiazine. Recoveries obtained ranged from 93 to 108%. The analysis of field samples obtained from seven commercial poultry flocks confirmed the adequacy of the method since it detected means concentrations ranging from 20 to 10,364 μg kg⁻¹. This provides us an accurate and reliable tool to monitor AMs residues in poultry litter and control its use as agricultural fertilizer. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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13 pages, 2173 KiB

Open AccessArticle

Evaluation of Antibiotic Dissemination into the Environment and Untreated Animals, by Analysis of Oxytetracycline in Poultry Droppings and Litter

by Ekaterina Pokrant, Karina Yévenes, Lina Trincado, Gigliola Terraza, Nicolás Galarce, Aldo Maddaleno, Betty San Martín, Lisette Lapierre and Javiera Cornejo

Animals 2021, 11(3), 853; https://doi.org/10.3390/ani11030853 - 17 Mar 2021

Cited by 10 | Viewed by 11270

Abstract

Oxytetracycline (OTC) is widely used in broiler chickens. During and after treatment a fraction of OTC is excreted in its original form and as its epimer, 4-epi-OTC in droppings. To address the transfer of OTC into the environment, we evaluated the dissemination of [...] Read more.

Oxytetracycline (OTC) is widely used in broiler chickens. During and after treatment a fraction of OTC is excreted in its original form and as its epimer, 4-epi-OTC in droppings. To address the transfer of OTC into the environment, we evaluated the dissemination of OTC and 4-epi-OTC from treated birds to the environment and sentinels, through the simultaneous analysis of broiler droppings and litter. Male broiler chickens were bred in controlled conditions. One group was treated by orogastric tube with 80 mg kg⁻¹ of OTC and two groups received no treatment (sentinels). OTC+4-epi-OTC were analyzed and detected by a HPLC-MS/MS post the end of treatment. The highest concentrations of OTC+4-epi-OTC were detected in the droppings of treated birds 14-days following the end of treatment (2244.66 µg kg⁻¹), and one day following the end of treatment in the litter (22,741.68 µg kg⁻¹). Traces of OTC+4-epi-OTC were detected in the sentinels’ droppings and litter (<12.2 µg kg⁻¹). OTC+4-epi-OTC can be transferred from treated birds to the environment and to other untreated birds. The presence and persistence of OTC+4-epi-OTC in litter could contribute to the selection of resistant bacteria in the environment, increasing the potential hazard to public and animal health. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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11 pages, 895 KiB

Open AccessArticle

Effects of Decabrominated Diphenyl Ether Exposure on Growth, Meat Characteristics and Blood Profiles in Broilers

by Zehui Liu, Hulong Lei, Renyong Tang, Junhua Yang, Xiulan Guo, Renmao Huang, Qinxiong Rao, Lin Cheng and Zhihui Zhao

Animals 2021, 11(2), 565; https://doi.org/10.3390/ani11020565 - 22 Feb 2021

Cited by 4 | Viewed by 2463

Abstract

Decabrominated diphenyl ether (BDE-209) is widely used as a flame retardant and is detected at high levels in the environment. Its toxicities have been reported and have attracted attention. In the present study, broilers were used to determine the response in growth performance, [...] Read more.

Decabrominated diphenyl ether (BDE-209) is widely used as a flame retardant and is detected at high levels in the environment. Its toxicities have been reported and have attracted attention. In the present study, broilers were used to determine the response in growth performance, carcass traits, meat quality, blood profiles and antioxidant system to BDE-209 exposure at doses of 0, 0.02, 0.4 and 4 mg/kg. The results showed that BDE-209 exposure at levels of 0.02 or 0.4 mg/kg increased feed intake and decreased feed efficiency. BDE-209 altered the blood profiles, such as reducing the numbers of white blood cells, lymphocytes and neutrophilic granulocytes. As compared with the control, BDE-209 exposure significantly increased abdominal fat percentages of broilers at 64.9–159.5% and adversely affected the selected biochemical indicators, including alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine (CRE), which indicated its toxicity to liver and kidney functions. Moreover, BDE-209 exposure significantly increased plasma malondialdehyde (MDA) concentrations and decreased the activities of glutathione peroxidase (GSH-px) and superoxide dismutase (SOD), which implied aggravating oxidant stress and decline of antioxidant capacity in broilers. In conclusion, our data demonstrated that the environmental pollutant BDE-209 adversely influenced growth performance, increased the deposition of abdominal fat, impaired antioxidant capacity and the immune system and had potential toxicity to the liver and kidney of broilers. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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Review

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8 pages, 2573 KiB

Open AccessReview

COVID-19 in Farm Animals: Host Susceptibility and Prevention Strategies

by Sachin Subedi, Sulove Koirala and Lilong Chai

Animals 2021, 11(3), 640; https://doi.org/10.3390/ani11030640 - 28 Feb 2021

Cited by 7 | Viewed by 3940

Abstract

COVID-19 is caused by the virus SARS-CoV-2 that belongings to the family of Coronaviridae, which has affected multiple species and demonstrated zoonotic potential. The COVID-19 infections have been reported on farm animals (e.g., minks) and pets, which were discussed and summarized in this [...] Read more.

COVID-19 is caused by the virus SARS-CoV-2 that belongings to the family of Coronaviridae, which has affected multiple species and demonstrated zoonotic potential. The COVID-19 infections have been reported on farm animals (e.g., minks) and pets, which were discussed and summarized in this study. Although the damage of COVID-19 has not been reported as serious as highly pathogenic avian influenza (HPAI) for poultry and African Swine Fever (ASF) for pigs on commercial farms so far, the transmission mechanism of COVID-19 among group animals/farms and its long-term impacts are still not clear. Prior to the marketing of efficient vaccines for livestock and animals, on-farm biosecurity measures (e.g., conventional disinfection strategies and innovated technologies) need to be considered or innovated in preventing the direct contact spread or the airborne transmission of COVID-19. Full article

(This article belongs to the Special Issue The Impact of Emerging Hazards in Animal Production)

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