A plant biostimulant (PBs) is any substance or microorganism applied to plants and intended to enhance nutrition efficiency, abiotic stress tolerance, and/or crop quality traits, regardless of its nutrient content. The first generation of organic plant biostimulants were complex mixtures obtained by extracting the existing organic fertilizer/soil improvers. The second generation of plant biostimulants are products based on the selected active ingredient. One of these active ingredients is chitosan. One of the problems is that chitosan is not water-soluble and cannot be applied easily to plants. Chemical covalent grafting of hydroxycinnamic acids could solve this problem and improve plant biostimulant activity. We investigated the grafting approach by flow chemistry and compared the results of the flow system with the batch reaction. We made batch and in-flow grafting reactions based on the radical grafting method. After the reaction, the conjugate was dialyzed for 3 days and lyophilized. Solubility was studied in water. The lyophilized grafted conjugates were characterized with FTIR, UV-Vis, TEM, and DLS and analyzed for their antioxidant activities. We compared the reaction performed in batch with the ones performed in flow chemistry. We determined the qualitative grafting of ferulic acid of dialyzed and lyophilized powder solubilized in water. The presence of absorbance at 287 and 310 nm corresponding to ferulic acid was observed on the hybrid [1]. The antioxidant activities were maintained for the flow chemistry reactions when compared to batch. FTIR showed an appearing peak corresponding to amide II groups, confirming the grafting. TEM images and DLS revealed the formation of particles with dimensions of around a few hundred nm.
Author Contributions
Conceptualization, F.O., D.C.-A. and I.S.H.; methodology, I.S.H., D.C.-A. and L.D., investigation, I.S.H., L.D., I.B. and O.D.; project administration, F.O.; supervision, F.O.; validation I.S.H., L.D. and I.B., visualization, I.S.H., writing—original draft preparation, I.S.H.; writing—review and editing, I.S.H. and F.O.; funding acquisition, F.O. All authors have read and agreed to the published version of the manuscript.
Funding
The research leading to these results has received funding from the NO Grants 2014–2021, under Project RO-NO-2019-540 STIM 4+, contract no. 14/2020.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Not applicable.
Acknowledgments
The authors thank Bogdan Trică for experimental support.
Conflicts of Interest
The authors declare no conflict of interest.
Reference
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