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High-Throughput, Volume 9, Issue 2

2020 June - 8 articles

Cover Story: The increasing interest in metagenomics is enhancing our knowledge regarding the composition and role of the microbiota in human physiology and pathology. Indeed, microbes have been reported to play a role in several diseases, including infertility. In particular, the male seminal microbiota has been suggested as an important factor able to influence couple’s health and pregnancy outcomes, as well as offspring health. Nevertheless, few studies have been carried out to date to deeper investigate semen microbiome origins and functions, and its correlations with the partner’s reproductive tract microbiome. Here, we report the state of the art regarding the male reproductive system microbiome and its alterations in infertility. View this paper.
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Articles (8)

  • Article
  • Open Access
16 Citations
6,529 Views
12 Pages
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Intra-Laboratory Evaluation of Luminescence Based High-Throughput Serum Bactericidal Assay (L-SBA) to Determine Bactericidal Activity of Human Sera against Shigella

  • Omar Rossi,
  • Eleonora Molesti,
  • Allan Saul,
  • Carlo Giannelli,
  • Francesca Micoli and
  • Francesca Necchi
High-Throughput2020, 9(2), 14;https://doi.org/10.3390/ht9020014

8 June 2020

Despite the huge decrease in deaths caused by Shigella worldwide in recent decades, shigellosis still causes over 200,000 deaths every year. No vaccine is currently available, and the morbidity of the disease coupled with the rise of antimicrobial re...

Full Article
  • Communication
  • Open Access
2 Citations
4,880 Views
8 Pages
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Genetic Counseling and NGS Screening for Recessive LGMD2A Families

  • Claudia Strafella,
  • Valerio Caputo,
  • Giulia Campoli,
  • Rosaria Maria Galota,
  • Julia Mela,
  • Stefania Zampatti,
  • Giulietta Minozzi,
  • Cristina Sancricca,
  • Serenella Servidei and
  • Raffaella Cascella
  • + 1 author
High-Throughput2020, 9(2), 13;https://doi.org/10.3390/ht9020013

10 May 2020

Genetic counseling applied to limb–girdle muscular dystrophies (LGMDs) can be very challenging due to their clinical and genetic heterogeneity and the availability of different molecular assays. Genetic counseling should therefore be addressed...

Full Article
  • Review
  • Open Access
64 Citations
15,005 Views
15 Pages
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Microbiota and Human Reproduction: The Case of Female Infertility

  • Rossella Tomaiuolo,
  • Iolanda Veneruso,
  • Federica Cariati and
  • Valeria D’Argenio
High-Throughput2020, 9(2), 12;https://doi.org/10.3390/ht9020012

3 May 2020

During the last decade, the availability of next-generation sequencing-based approaches has revealed the presence of microbial communities in almost all the human body, including the reproductive tract. As for other body sites, this resident microbio...

Full Article
  • Feature Paper
  • Article
  • Open Access
5,404 Views
20 Pages
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Chimeric Virus Made from crTMV RNA and the Coat Protein of Potato Leafroll Virus is Targeted to the Nucleolus and Can Infect Nicotiana benthamiana Mechanically

  • Konstantin O. Butenko,
  • Inna A. Chaban,
  • Eugene V. Skurat,
  • Olga A. Kondakova and
  • Yuri F. Drygin
High-Throughput2020, 9(2), 11;https://doi.org/10.3390/ht9020011

26 April 2020

A genetically engineered chimeric virus crTMV-CP-PLRV composed of the crucifer-infecting tobacco mosaic virus (crTMV) RNA and the potato leafroll virus (PLRV) coat protein (CP) was obtained by agroinfiltration of Nicotiana benthamiana with the binary...

Full Article
  • Feature Paper
  • Review
  • Open Access
43 Citations
9,958 Views
9 Pages
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Microbiota and Human Reproduction: The Case of Male Infertility

  • Rossella Tomaiuolo,
  • Iolanda Veneruso,
  • Federica Cariati and
  • Valeria D’Argenio
High-Throughput2020, 9(2), 10;https://doi.org/10.3390/ht9020010

13 April 2020

The increasing interest in metagenomics is enhancing our knowledge regarding the composition and role of the microbiota in human physiology and pathology. Indeed, microbes have been reported to play a role in several diseases, including infertility....

Full Article
  • Article
  • Open Access
8 Citations
4,903 Views
12 Pages
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A Simple, Label-Free, and High-Throughput Method to Evaluate the Epigallocatechin-3-Gallate Impact in Plasma Molecular Profile

  • Rúben Araújo,
  • Luís Ramalhete,
  • Helder Da Paz,
  • Edna Ribeiro and
  • Cecília R.C. Calado
High-Throughput2020, 9(2), 9;https://doi.org/10.3390/ht9020009

9 April 2020

Epigallocatechin-3-gallate (EGCG), the major catechin present in green tea, presents diverse appealing biological activities, such as antioxidative, anti-inflammatory, antimicrobial, and antiviral activities, among others. The present work evaluated...

Full Article
  • Feature Paper
  • Review
  • Open Access
9 Citations
6,300 Views
16 Pages
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DMETTM Genotyping: Tools for Biomarkers Discovery in the Era of Precision Medicine

  • Giuseppe Agapito,
  • Marzia Settino,
  • Francesca Scionti,
  • Emanuela Altomare,
  • Pietro Hiram Guzzi,
  • Pierfrancesco Tassone,
  • Pierosandro Tagliaferri,
  • Mario Cannataro,
  • Mariamena Arbitrio and
  • Maria Teresa Di Martino
High-Throughput2020, 9(2), 8;https://doi.org/10.3390/ht9020008

29 March 2020

The knowledge of genetic variants in genes involved in drug metabolism may be translated into reduction of adverse drug reactions, increase of efficacy, healthcare outcomes improvement and economic benefits. Many high-throughput tools are available f...

Full Article
  • Feature Paper
  • Review
  • Open Access
49 Citations
11,065 Views
7 Pages
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Colonization Resistance in the Infant Gut: The Role of B. infantis in Reducing pH and Preventing Pathogen Growth

  • Rebbeca M. Duar,
  • David Kyle and
  • Giorgio Casaburi
High-Throughput2020, 9(2), 7;https://doi.org/10.3390/ht9020007

27 March 2020

Over the past century, there has been a steady increase in the stool pH of infants from industrialized countries. Analysis of historical data revealed a strong association between abundance of Bifidobacterium in the gut microbiome of breasted infants...

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