Evaluation of Cytotoxic Effects of Carnosic Acid Alone and Combination with Cisplatin in HepG2 Cells †
1
Graduate School of Health Sciences, Erciyes University, 38280 Kayseri, Turkey
2
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Erciyes University, 38280 Kayseri, Turkey
*
Author to whom correspondence should be addressed.
†
Presented at the 3rd International conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 18–20 December 2019.
Proceedings 2019, 40(1), 39; https://doi.org/10.3390/proceedings2019040039
Published: 31 December 2019
(This article belongs to the Proceedings of The 3rd International conference on Natural Products for Cancer Prevention and Therapy)
Abstract
:Natural products are important in prevention and treatment of cancer because of their antitumor effect and reducing side effects of chemotherapeutic drugs. The aim of this study was to investigate the potential cytotoxic effecs of carnosic acid and in combination with cisplatin in liver cancer cells. Cytotoxicity was assessed using MTT assay for 24/48 hours. The intracellular ROS levels were determined using the oxidation-sensitive fluorescent probes DCFH-DA. Changes in the mitochondrial membrane potential (MMP) were detected using JC-1 commercial kit. Concentrations were selected for carnosic acid and cisplatin according to IC50 values. As a results, % cell viability decreased with concentration/time dependent and combination treatments showed potentiated effect at 48 hours exposure. According to DCFH-DA assay, it was observed that carnosic acid and combinations with cisplatin reduced intracellular ROS levels in presence of H2O2. Carnosic acid and its combinations reduced MMP. Our results showed that carnosic acid has the potential to inhibit growth in HepG2 cells without increasing ROS production. In conclusion, carnosic acid alone and combination with cisplatin may be promising for the prevention and treatment of liver cancer.
Acknowledgments
This research was supported by Erciyes University Scientific Research Foundation (Project No: TYL-2018-8632.
© 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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MDPI and ACS Style
Akın, U.N.; Bakır, E.; Ökçesiz, A.; Eken, A. Evaluation of Cytotoxic Effects of Carnosic Acid Alone and Combination with Cisplatin in HepG2 Cells. Proceedings 2019, 40, 39. https://doi.org/10.3390/proceedings2019040039
AMA Style
Akın UN, Bakır E, Ökçesiz A, Eken A. Evaluation of Cytotoxic Effects of Carnosic Acid Alone and Combination with Cisplatin in HepG2 Cells. Proceedings. 2019; 40(1):39. https://doi.org/10.3390/proceedings2019040039
Chicago/Turabian StyleAkın, Uğur Nuri, Elçin Bakır, Aysun Ökçesiz, and Ayşe Eken. 2019. "Evaluation of Cytotoxic Effects of Carnosic Acid Alone and Combination with Cisplatin in HepG2 Cells" Proceedings 40, no. 1: 39. https://doi.org/10.3390/proceedings2019040039
