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Abstract

The Cytotoxic and Apoptotic Effects of Usnic Acid on Prostate Cancer versus Normal Cells †

1
Medical Biology Department, Faculty of Medicine, Uludag University, Bursa 16059, Turkey
2
Medical Biology Department, Faculty of Medicine, Sakarya University, Sakarya 54050, Turkey
*
Author to whom correspondence should be addressed.
Presented at the 2nd International Conference on Natural Products for Cancer Prevention and Therapy, Kayseri, Turkey, 8–11 November, 2017.
Proceedings 2017, 1(10), 1027; https://doi.org/10.3390/proceedings1101027
Published: 15 November 2017

Abstract

:
Prostate cancer (PC) is the most common epithelium-derived solid tissue cancer in males in developed countries. Lichen seconder metabolites have an important potential as new generation chemotherapeutics. Therefore, we evaluated the cytotoxic and apoptotic effects of a lichen seconder metabolite, usnic acid (UA), on androgen receptor-positive PC cells (LNCaP) and also prostate normal cell line (RWPE-1) using WST-1 proliferation assay, acridine orange/ethidium bromide (AO/EB) staining, and annexin V analysis. The viability of LNCaP cells was significantly reduced (p < 0.05) after exposure to increasing concentrations of UA (6.25–150 µM). The LNCaP cell viability decreased to 34.14%, whereas RWPE-1 viability ratio was 109.79% at 150 µM UA for 48 h. UA induced especially early apoptosis in LNCaP cells and total apoptotic cell ratio was 69.01% at 150 µM for 48 h. Apoptotic morphology was also detected with AO/EB in LNCaP cells but not in RWPE-1 cells. Consequently, UA displayed more cytotoxic and apoptotic effects on PC cells than prostate normal cells. Thus, we suggest that UA might be one of the important lichen metabolites in further investigations for prostate cancer therapy.

Conflicts of Interest

The authors declare no conflict of interest.
Figure 1. Morphological changes in LNCaP (A) and RWPE-1 (B) cells after 150 µM UA treatment for 48 h.
Figure 1. Morphological changes in LNCaP (A) and RWPE-1 (B) cells after 150 µM UA treatment for 48 h.
Proceedings 01 01027 g001
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MDPI and ACS Style

Eryılmaz, I.E.; Eskiler, G.G.; Yurdacan, B.; Egeli, Ü.; Çeçener, G.; Tunca, B. The Cytotoxic and Apoptotic Effects of Usnic Acid on Prostate Cancer versus Normal Cells. Proceedings 2017, 1, 1027. https://doi.org/10.3390/proceedings1101027

AMA Style

Eryılmaz IE, Eskiler GG, Yurdacan B, Egeli Ü, Çeçener G, Tunca B. The Cytotoxic and Apoptotic Effects of Usnic Acid on Prostate Cancer versus Normal Cells. Proceedings. 2017; 1(10):1027. https://doi.org/10.3390/proceedings1101027

Chicago/Turabian Style

Eryılmaz, Işıl Ezgi, Gamze Güney Eskiler, Beste Yurdacan, Ünal Egeli, Gülşah Çeçener, and Berrin Tunca. 2017. "The Cytotoxic and Apoptotic Effects of Usnic Acid on Prostate Cancer versus Normal Cells" Proceedings 1, no. 10: 1027. https://doi.org/10.3390/proceedings1101027

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