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Methods and Protocols, Volume 6, Issue 1

2023 February - 20 articles

Cover Story: Reliability of serotonin (5-HT) electrochemical measurements suffers due to long adsorption times and electrode fouling, which occurs as 5-HT oxidation byproduct buildup on the electrode surface, gradually reducing the ability to detect new molecules. Presented here is a computational method to track molecular adsorption and detection processes, which lead to fouling by combining Langmuir adsorption kinetic equations and adsorption-limited electrochemical equations, which have not yet been combined for this purpose. This method was used to predict signals generated from electrochemical 5-HT detection under varying conditions, fouling over 50 cycles, and detection of cell-secreted 5-HT. Further, this method could be applied to many adsorption-limited electrochemical analytes and electrode types to improve modeling and optimization. View this paper
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Articles (20)

  • Article
  • Open Access
2,216 Views
10 Pages

On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging

  • Sergi Mallorca-Cebria,
  • Yolanda Moliner-Martinez,
  • Carmen Molins-Legua and
  • Pilar Campins-Falcó

14 February 2023

The potential of antioxidants in preventing several diseases has attracted great attention in recent years. Indeed, these products are part of a multi-billion industry. However, there is a lack of scientific information about safety, quality, doses,...

  • Protocol
  • Open Access
6 Citations
3,882 Views
15 Pages

Augmenting Mental Health Support for Patients Accessing Different Degrees of Formal Psychiatric Care through a Supportive Text Messaging Program: Protocol for a Randomized Controlled Trial

  • Raquel da Luz Dias,
  • Reham Shalaby,
  • Belinda Agyapong,
  • Gloria Obuobi-Donkor,
  • Medard K. Adu,
  • Ejemai Eboreime,
  • Samuel Obeng Nkrumah,
  • Sanjana Sridharan,
  • Patryk Simon and
  • Vincent Israel Opoku Agyapong
  • + 29 authors

13 February 2023

Patients feel more vulnerable when accessing community mental health programs for the first time or after being discharged from psychiatric inpatient units. Long wait times for follow-up appointments, shortage of mental health professionals, lack of...

  • Protocol
  • Open Access
12 Citations
6,314 Views
13 Pages

12 February 2023

Flow cytometry gives a unique opportunity to analyze thousands of individual cells for multiple parameters in a course of minutes. The most commonly used flow cytometry application in plant biology is estimation of nuclear DNA content. This becomes a...

  • Protocol
  • Open Access
11 Citations
4,809 Views
12 Pages

8 February 2023

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous organic compounds coming from natural or anthropogenic activities. Tree organs such as leaves and barks have been used to monitor urban air quality and have achieved remarkable ecological importa...

  • Protocol
  • Open Access
9 Citations
3,135 Views
13 Pages

Search and Identification of Amyloid Proteins

  • Tatyana A. Belashova,
  • Anna A. Valina,
  • Evgeniy I. Sysoev,
  • Maria E. Velizhanina,
  • Andrew A. Zelinsky and
  • Alexey P. Galkin

4 February 2023

Amyloids are fibrillar proteins with a cross-β structure. Pathological amyloids are associated with the development of a number of incurable diseases, while functional amyloids regulate vital processes. The detection of unknown amyloids in livin...

  • Protocol
  • Open Access
8 Citations
3,573 Views
9 Pages

A Production and Fractionation Protocol for Polyvinyl Chloride Microplastics

  • Siebe Lievens,
  • Evelynn Vervoort,
  • Giulia Poma,
  • Adrian Covaci and
  • Mik Van Der Borght

31 January 2023

Concerns about the presence of microplastics in the environment has increased in recent years, prompting more attention from scientists. Thorough exposure studies using artificially produced microplastics containing additives are required to assess t...

  • Protocol
  • Open Access
8 Citations
9,697 Views
8 Pages

Optimized Protocol for Isolation and Culture of Murine Neonatal Primary Lung Fibroblasts

  • Rocío Fuentes-Mateos,
  • Eugenio Santos and
  • Alberto Fernández-Medarde

24 January 2023

During all the stages of lung development, the lung mesoderm (or mesenchyme) is closely related to the endoderm, and their cross-regulation promotes, controls, and drives all lung developmental processes. Generation of 3D organoids in vitro, RNA assa...

  • Study Protocol
  • Open Access
3 Citations
2,472 Views
6 Pages

24 January 2023

Introduction: Pre-diabetes is an intermediate, asymptomatic state between normoglycaemia and the onset of type 2 diabetes mellitus (T2D). Recent reports indicate that there are sub-clinical changes observed in red blood cells during pre-diabetes. Thi...

  • Protocol
  • Open Access
2 Citations
4,510 Views
13 Pages

Protocol for a Randomized Control Trial for Tungiasis Treatment in Homa Bay County, Kenya: Dimeticone versus Sodium Carbonate

  • Kana Suzuki,
  • Yasuhiko Kamiya,
  • Chris Smith,
  • Satoshi Kaneko,
  • Asiko Ongaya and
  • Evans Amukoye

23 January 2023

Tungiasis, a World Health Organization neglected tropical disease, is caused by the female sand flea. Most clinical trials for tungiasis use expensive or impractical drugs, which are difficult for residents to use. However, in western Kenya, communit...

  • Article
  • Open Access
5 Citations
6,263 Views
13 Pages

23 January 2023

Hydrolysis of protein samples into amino acids facilitates the use of NMR spectroscopy for protein and peptide quantification. Different conditions have been tested for quantifying aromatic amino acids and proteins. The pH-dependent signal shifts in...

  • Protocol
  • Open Access
20 Citations
10,907 Views
17 Pages

Ion Selective Electrode (ISE) Method for Determination of Total Fluorine and Total Organic Fluorine in Packaging Substrates

  • Ma Cristine Concepcion D. Ignacio,
  • Greg W. Curtzwiler,
  • Mark R. Early,
  • Katie M. Updegraff and
  • Keith L. Vorst

18 January 2023

Various testing methods and techniques have been used to identify and quantify per- and polyfluoroalkyl substances (PFAS) in food packaging. A common indirect measurement of PFAS is total fluorine (TF) and total organic fluorine (TOF). These methods...

  • Protocol
  • Open Access
7 Citations
4,245 Views
16 Pages

Advanced Flow Cytometry Using the SYTO-13 Dye for the Assessment of Platelet Reactivity and Maturity in Whole Blood

  • Oliver Buchhave Pedersen,
  • Leonardo Pasalic,
  • Erik Lerkevang Grove,
  • Steen Dalby Kristensen,
  • Anne-Mette Hvas and
  • Peter H. Nissen

Newly produced immature platelets are larger, contain higher amounts of residual RNA, and are more reactive than mature platelets. Flow cytometry using the SYTO-13 dye is a method for the subdivision of immature platelets from mature platelets based...

  • Protocol
  • Open Access
2 Citations
3,983 Views
11 Pages

Efficacy and Long-Term Safety of Ibuprofen Gargle for Oral Lichen Planus: A Study Protocol of Randomized Crossover and Long-Term Extension Trials

  • Yumi Kitahiro,
  • Takeshi Ioroi,
  • Yasumasa Kakei,
  • Junya Yamashita,
  • Akira Kimoto,
  • Takumi Hasegawa,
  • Asami Morioka,
  • Kazuhiro Yamamoto,
  • Masaya Akashi and
  • Ikuko Yano

Oral lichen planus (OLP) is a type of chronic and refractory stomatitis characterized by abnormal keratinization, which is often painful. There is no consensus regarding treatment options for OLP, particularly in the presence of pain. The current stu...

  • Article
  • Open Access
4 Citations
3,738 Views
15 Pages

Serotonin (5-HT) is a neurotransmitter involved in many biophysiological processes in the brain and in the gastrointestinal tract. Electrochemical methods are commonly used to quantify 5-HT, but their reliability may suffer due to the time-dependent...

  • Protocol
  • Open Access
1 Citations
3,647 Views
12 Pages

COVID-19 Related Protocol Considerations and Modifications within a Rural, Community-Engaged Health Promotion Randomized Trial

  • Rebecca A. Seguin-Fowler,
  • Galen D. Eldridge,
  • Meredith Graham,
  • Sara C. Folta,
  • Karla L. Hanson and
  • Jay E. Maddock

Rural communities are at higher risk for physical inactivity, poor dietary behaviors, and related chronic diseases and obesity. These disparities are largely driven by built environment, socioeconomic, and social factors. A community-based cluster ra...

  • Protocol
  • Open Access
1 Citations
3,924 Views
9 Pages

Analytical Validation of SOD2 Genotyping

  • Marija Debeljak,
  • Stacy Riel,
  • Ming-Tseh Lin,
  • James R. Eshleman and
  • Channing J. Paller

31 December 2022

Manganese superoxide dismutase-2 (SOD2) plays a crucial role in cells’ protection against mitochondrial oxidative damage. A genetic polymorphism in the mitochondrial targeting sequence of the SOD2 gene has been implicated in various diseases, i...

  • Protocol
  • Open Access
3 Citations
5,350 Views
10 Pages

Isolation and Cultivation of Adipose-Derived Mesenchymal Stem Cells Originating from the Infrapatellar Fat Pad Differentiated with Blood Products: Method and Protocol

  • Markus Neubauer,
  • Karina Kramer,
  • Johannes Neugebauer,
  • Lukas Moser,
  • Anna Moser,
  • Dietmar Dammerer and
  • Stefan Nehrer

25 December 2022

Adipose-derived mesenchymal stem cells (ASCs) are a promising source for clinical application in regenerative orthopedics. ASCs derived from the infra-patellar fat pad (IFP)—a distinct adipose structure in the knee—show superior regenerat...

  • Protocol
  • Open Access
1 Citations
2,881 Views
9 Pages

24 December 2022

The gut microbiota has been immensely studied over the past years because of its involvement in the pathogenesis of numerous diseases. However, gut microbiota data in Africa are limited. Therefore, it is crucial to have studies that reflect various p...

  • Article
  • Open Access
7 Citations
4,506 Views
16 Pages

Engineering Ag43 Signal Peptides with Bacterial Display and Selection

  • Darius Wen-Shuo Koh,
  • Jian-Hua Tay and
  • Samuel Ken-En Gan

23 December 2022

Protein display, secretion, and export in prokaryotes are essential for utilizing microbial systems as engineered living materials, medicines, biocatalysts, and protein factories. To select for improved signal peptides for Escherichia coli protein di...

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Methods Protoc. - ISSN 2409-9279