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Methods and Protocols, Volume 6, Issue 1

February 2023 - 20 articles

Cover Story: Reliability of serotonin (5-HT) electrochemical measurements suffers due to long adsorption times and electrode fouling, which occurs as 5-HT oxidation byproduct buildup on the electrode surface, gradually reducing the ability to detect new molecules. Presented here is a computational method to track molecular adsorption and detection processes, which lead to fouling by combining Langmuir adsorption kinetic equations and adsorption-limited electrochemical equations, which have not yet been combined for this purpose. This method was used to predict signals generated from electrochemical 5-HT detection under varying conditions, fouling over 50 cycles, and detection of cell-secreted 5-HT. Further, this method could be applied to many adsorption-limited electrochemical analytes and electrode types to improve modeling and optimization. View this paper
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Articles (20)

  • Article
  • Open Access
2,141 Views
10 Pages

On-Site Multisample Determination of Chlorogenic Acid in Green Coffee by Chemiluminiscent Imaging

  • Sergi Mallorca-Cebria,
  • Yolanda Moliner-Martinez,
  • Carmen Molins-Legua and
  • Pilar Campins-Falcó

14 February 2023

The potential of antioxidants in preventing several diseases has attracted great attention in recent years. Indeed, these products are part of a multi-billion industry. However, there is a lack of scientific information about safety, quality, doses,...

  • Protocol
  • Open Access
5 Citations
3,663 Views
15 Pages

Augmenting Mental Health Support for Patients Accessing Different Degrees of Formal Psychiatric Care through a Supportive Text Messaging Program: Protocol for a Randomized Controlled Trial

  • Raquel da Luz Dias,
  • Reham Shalaby,
  • Belinda Agyapong,
  • Gloria Obuobi-Donkor,
  • Medard K. Adu,
  • Ejemai Eboreime,
  • Samuel Obeng Nkrumah,
  • Sanjana Sridharan,
  • Patryk Simon and
  • Bryanne Taylor
  • + 29 authors

13 February 2023

Patients feel more vulnerable when accessing community mental health programs for the first time or after being discharged from psychiatric inpatient units. Long wait times for follow-up appointments, shortage of mental health professionals, lack of...

  • Protocol
  • Open Access
12 Citations
5,877 Views
13 Pages

12 February 2023

Flow cytometry gives a unique opportunity to analyze thousands of individual cells for multiple parameters in a course of minutes. The most commonly used flow cytometry application in plant biology is estimation of nuclear DNA content. This becomes a...

  • Protocol
  • Open Access
10 Citations
4,591 Views
12 Pages

8 February 2023

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous organic compounds coming from natural or anthropogenic activities. Tree organs such as leaves and barks have been used to monitor urban air quality and have achieved remarkable ecological importa...

  • Protocol
  • Open Access
9 Citations
3,014 Views
13 Pages

Search and Identification of Amyloid Proteins

  • Tatyana A. Belashova,
  • Anna A. Valina,
  • Evgeniy I. Sysoev,
  • Maria E. Velizhanina,
  • Andrew A. Zelinsky and
  • Alexey P. Galkin

4 February 2023

Amyloids are fibrillar proteins with a cross-β structure. Pathological amyloids are associated with the development of a number of incurable diseases, while functional amyloids regulate vital processes. The detection of unknown amyloids in livin...

  • Protocol
  • Open Access
7 Citations
3,434 Views
9 Pages

A Production and Fractionation Protocol for Polyvinyl Chloride Microplastics

  • Siebe Lievens,
  • Evelynn Vervoort,
  • Giulia Poma,
  • Adrian Covaci and
  • Mik Van Der Borght

31 January 2023

Concerns about the presence of microplastics in the environment has increased in recent years, prompting more attention from scientists. Thorough exposure studies using artificially produced microplastics containing additives are required to assess t...

  • Protocol
  • Open Access
5 Citations
9,261 Views
8 Pages

Optimized Protocol for Isolation and Culture of Murine Neonatal Primary Lung Fibroblasts

  • Rocío Fuentes-Mateos,
  • Eugenio Santos and
  • Alberto Fernández-Medarde

24 January 2023

During all the stages of lung development, the lung mesoderm (or mesenchyme) is closely related to the endoderm, and their cross-regulation promotes, controls, and drives all lung developmental processes. Generation of 3D organoids in vitro, RNA assa...

  • Study Protocol
  • Open Access
3 Citations
2,402 Views
6 Pages

24 January 2023

Introduction: Pre-diabetes is an intermediate, asymptomatic state between normoglycaemia and the onset of type 2 diabetes mellitus (T2D). Recent reports indicate that there are sub-clinical changes observed in red blood cells during pre-diabetes. Thi...

  • Protocol
  • Open Access
2 Citations
4,254 Views
13 Pages

Protocol for a Randomized Control Trial for Tungiasis Treatment in Homa Bay County, Kenya: Dimeticone versus Sodium Carbonate

  • Kana Suzuki,
  • Yasuhiko Kamiya,
  • Chris Smith,
  • Satoshi Kaneko,
  • Asiko Ongaya and
  • Evans Amukoye

23 January 2023

Tungiasis, a World Health Organization neglected tropical disease, is caused by the female sand flea. Most clinical trials for tungiasis use expensive or impractical drugs, which are difficult for residents to use. However, in western Kenya, communit...

  • Article
  • Open Access
5 Citations
5,918 Views
13 Pages

23 January 2023

Hydrolysis of protein samples into amino acids facilitates the use of NMR spectroscopy for protein and peptide quantification. Different conditions have been tested for quantifying aromatic amino acids and proteins. The pH-dependent signal shifts in...

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Methods Protoc. - ISSN 2409-9279