Next Article in Journal
Series of Chloranilate-Bridged Dinuclear Lanthanide Complexes: Kramers Systems Showing Field-Induced Slow Magnetic Relaxation
Next Article in Special Issue
Disturbance of the Conformation of DNA Hairpin Containing the 5′-GT-3′ Binding Site Caused by Zn(II)bleomycin-A5 Studied through NMR Spectroscopy
Previous Article in Journal
Bilayer Thin Films That Combine Luminescent and Spin Crossover Properties for an Efficient and Reversible Fluorescence Switching
Open AccessCommunication

Sensitive Water Ligand Observed via Gradient Spectroscopy with 19F Detection for Analysis of Fluorinated Compounds Bound to Proteins

1
Division of Agriculture and Agricultural Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
2
Department of Chemistry, College of Science and Technology, Meisei University, Hino, Tokyo 191-8506, Japan
*
Author to whom correspondence should be addressed.
Magnetochemistry 2019, 5(2), 29; https://doi.org/10.3390/magnetochemistry5020029
Received: 22 March 2019 / Revised: 9 April 2019 / Accepted: 24 April 2019 / Published: 1 May 2019
(This article belongs to the Special Issue Nuclear Magnetic Resonance Spectroscopy in Biomedical Application)
The water ligand observed via a gradient spectroscopy type experiment with 19F detection was applied to selectively detect fluorinated compounds with affinity to the target proteins. The 19F signals of bound and unbound compounds were observed as opposite phases, which was advantageous to distinguish the binding state. The proposed NMR method was optimized based on the 19F{1H} saturation transfer difference pulse sequence, and various inversion pulses for the water resonance were evaluated with the aim of high sensitivity. View Full-Text
Keywords: 19F NMR; NMR-based screening; fluorinated compound; water ligand observed via gradient spectroscopy (WaterLOGSY) 19F NMR; NMR-based screening; fluorinated compound; water ligand observed via gradient spectroscopy (WaterLOGSY)
Show Figures

Graphical abstract

MDPI and ACS Style

Furihata, K.; Tashiro, M. Sensitive Water Ligand Observed via Gradient Spectroscopy with 19F Detection for Analysis of Fluorinated Compounds Bound to Proteins. Magnetochemistry 2019, 5, 29.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop