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Volume 10
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10.3390/fermentation10010058
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Article

A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2

by
Zdravka Koleva
1,
Radoslav Abrashev
2,
Maria Angelova
2,
Galina Stoyancheva
3,
Boryana Spassova
2,
Lyudmila Yovchevska
2,
Vladislava Dishliyska
2,
Jeny Miteva-Staleva
2 and
Ekaterina Krumova
2,*
1
Department of Natural Sciences, New Bulgarian University, 1618 Sofia, Bulgaria
2
Department of Mycology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 26, 1113 Sofia, Bulgaria
3
Department of General Microbiology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 26, 1113 Sofia, Bulgaria
*
Author to whom correspondence should be addressed.
Fermentation 2024, 10(1), 58; https://doi.org/10.3390/fermentation10010058
Submission received: 5 December 2023 / Revised: 11 January 2024 / Accepted: 13 January 2024 / Published: 15 January 2024
(This article belongs to the Special Issue New Research on Strains Improvement and Microbial Biosynthesis)
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Abstract

Catalase (CAT) is an enzyme involved in the first line of cellular antioxidant defense. It plays a key role in the protection of a wide range of Antarctic organisms against cold stress. Extracellular catalase is very rare and data on it are extremely scarce. The aim of the present study was to select an efficient producer of extracellular catalase from amongst Antarctic filamentous fungi. Sixty-two Antarctic filamentous fungal strains were investigated for their potential ability to synthesize intracellular and extracellular CAT. The Antarctic strain Penicillium rubens III11-2 was selected as the best producer of extracellular catalase. New information on the involvement of the extracellular antioxidant enzymes superoxide dismutase and CAT in the response of filamentous fungi against low-temperature stress was obtained. An efficient scheme for the purification of CAT from culture fluid was developed. An enzyme preparation with high specific activity (513 U/mg protein) was obtained with a yield of 19.97% and a purification rate of 98.4-fold. The purified enzyme exhibited maximal enzymatic activity in the temperature range of 5–40 °C and temperature stability between 0 and 30 °C, therefore being characterized as temperature sensitive. To our knowledge, this is the first purified extracellular cold active catalase preparation from Antarctic filamentous fungi.
Keywords: Antarctic; filamentous fungi; extracellular catalase; Penicillium rubens Antarctic; filamentous fungi; extracellular catalase; Penicillium rubens

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MDPI and ACS Style

Koleva, Z.; Abrashev, R.; Angelova, M.; Stoyancheva, G.; Spassova, B.; Yovchevska, L.; Dishliyska, V.; Miteva-Staleva, J.; Krumova, E. A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2. Fermentation 2024, 10, 58. https://doi.org/10.3390/fermentation10010058

AMA Style

Koleva Z, Abrashev R, Angelova M, Stoyancheva G, Spassova B, Yovchevska L, Dishliyska V, Miteva-Staleva J, Krumova E. A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2. Fermentation. 2024; 10(1):58. https://doi.org/10.3390/fermentation10010058

Chicago/Turabian Style

Koleva, Zdravka, Radoslav Abrashev, Maria Angelova, Galina Stoyancheva, Boryana Spassova, Lyudmila Yovchevska, Vladislava Dishliyska, Jeny Miteva-Staleva, and Ekaterina Krumova. 2024. "A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2" Fermentation 10, no. 1: 58. https://doi.org/10.3390/fermentation10010058

APA Style

Koleva, Z., Abrashev, R., Angelova, M., Stoyancheva, G., Spassova, B., Yovchevska, L., Dishliyska, V., Miteva-Staleva, J., & Krumova, E. (2024). A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2. Fermentation, 10(1), 58. https://doi.org/10.3390/fermentation10010058

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