A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2
Abstract
:1. Introduction
2. Materials and Methods
2.1. Microorganisms and Identification
Molecular Methods for Identification of Isolates
2.2. Screening for Catalase Activity
2.2.1. Cultivation on Solid Media
2.2.2. Submerged Cultivation
2.3. Analytical Methods—Determination of Enzyme Activities, Sugars, Protein, Dry Weight
2.4. Isolation and Purification of Temperature-Sensitive Catalase
2.5. Determination of Temperature Optimum and Temperature Stability of the Purified Enzyme
2.5.1. Determination of Temperature Optimum
2.5.2. Determination of Temperature Stability
2.6. Statistical Evaluation of the Results
3. Results
3.1. Investigation on the Potential of Antarctic Filamentous Fungi for the Catalase Synthesis
3.2. Identification of Extracellular Catalase-Producing Strains
3.3. Selection of an Extracellular Catalase-Producing Strain
3.4. Investigation of the Role of Extracellular Catalase under Normoxic Conditions and under Low-Temperature Stress
3.5. Purification and Characterization of CA CAT
3.5.1. Preparation of Purified Enzyme
3.5.2. Properties of the Purified Enzyme Preparation
4. Discussion
5. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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N | Strain | Extracell. CAT (U/mg) | Intracell. CAT (U/mg) | N | Strain | Extracell. CAT (U/mg) | Intracell. CAT (U/mg) | N | Strain | Extracell. CAT (U/mg) | Intracell. CAT (U/mg) |
---|---|---|---|---|---|---|---|---|---|---|---|
1. | P27 | 2.5 | 22.9 | 22. | I 9 | 3.09 | 5.72 | 43. | I 13 | 4.25 | 8.7 |
2. | P22 | 3.6 | 16.5 | 23. | III 21 | 3.20 | 8.16 | 44. | I 112 | 2.27 | 4.7 |
3. | III 63 | 2.3 | 14.7 | 24. | III 71 | 1.33 | 19.54 | 45. | I 111 | 3.5 | 33.9 |
4. | P21 | 0.6 | 11.3 | 25. | III 81 | 6.96 | 31.04 | 46. | I 141 | 7.3 | 15.4 |
5. | P29 | 3.9 | 28.9 | 26. | III 83 | 1.70 | 26.72 | 47. | 1 11 | 1.7 | 6.8 |
6. | M12 | 0 | 16.4 | 27. | I 141 | 3.76 | 4.89 | 48. | I BH | 1.6 | 15.0 |
7. | P44 | 1.5 | 9.4 | 28. | III 22 | 0 | 6.32 | 49. | I 15 | 2.2 | 31.1 |
8. | P31 | 0 | 35.7 | 29. | III 63 | 10.27 | 19.97 | 50. | I 82 | 2.7 | 33.2 |
9. | P33 | trace | 5.9 | 30. | III 221 | 0 | trace | 51. | I 9 | 3.9 | 39.2 |
10. | M5 | 0.9 | 10.4 | 31. | III 67 | 2.04 | 17.55 | 52. | I 8 | 0.8 | 18.9 |
11. | II 62 | 2.3 | 10.0 | 32. | II 51 | 1.42 | 12.39 | 53. | II 43 | 1.81 | 17.23 |
12. | II 65 | 3.0 | 15.3 | 33. | III 112 | 8.19 | 40.21 | 54. | I 73 | 1.77 | 4.38 |
13. | II 66 | 1.1 | 14.9 | 34. | K1 | 2.36 | 1.61 | 55. | I 16 | 3.5 | 33.1 |
14. | II 51 | 1.9 | 12.2 | 35. | K7 | 1.79 | trace | 56. | I 10 | 1.6 | 21.0 |
15. | T35 | 1.6 | 15.8 | 36. | I 141 | 3 | 5.52 | 57. | I 19 | 1.3 | 22.0 |
16. | 119 | 1.3 | 13.4 | 37. | 15 | 1.93 | 19.97 | 58. | I 72 | 3.4 | 36.9 |
17. | I 13 | 3.74 | 6.8 | 38. | 16 | 0.86 | 23.94 | 59. | I 92 | 2.5 | 6.02 |
18. | I 21 | 0 | 9.5 | 39. | 19 | 1.34 | 9.75 | 60. | I 21 | 4.8 | 7.1 |
19. | I 16 | 3.3 | 5.7 | 40. | 22 | 0 | 10.18 | 61. | 1 71 | 2.7 | 27.4 |
20. | I 112 | 3.12 | 3.8 | 41. | I S | 2.43 | 5.29 | 62. | I 112 | 2.6 | 17.5 |
21. | I 101 | 0.89 | 11.9 | 42. | Б32 | 1.64 | 12.09 |
№ | Strain Abbreviation | Strain Name | Macroscopic Picture | Taxonomy | NCBI GenBank Database Accession Numbers |
---|---|---|---|---|---|
1. | P29 | Penicillium griseofulvum | Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Penicillium | MT722118 | |
2. | P22 | Penicillium sp. | Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Penicillium | MT730073 | |
3. | I1-6 | Penicillium rubens | Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Penicillium | MT758185 | |
4. | I9 | Aspergillus fumigatus | Class: Eurotiomycetes Order: Eurotiales Family: Aspergillaceae Genus: Aspergillus | MT758189 | |
5. | I11-2 | Penicillium rubens | Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Penicillium | MT722143 | |
6. | I13 | Penicillium commune | Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Penicillium | MT730061 | |
7. | I14-1 | Pseudogymnoascus pannorum | Class: Leotiomycetes Order: Helotiales Family: Myxotrichaceae Genus: Geomyces | MT722119 | |
8. | III2-1 | Epicoccum nigrum | Class: Dothideomycetes Order: Pleosporales Family: Didymellaceae Genus: Epicoccum | OR844311 | |
9. | III6-3 | Aspergillus glaucus | Class: Eurotiomycetes Order: Eurotiales Family: Aspergillaceae Genus: Aspergillus | JN206683.1 | |
10. | III8-1 | Penicillium commune | Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Penicillium | MT722126 | |
11. | III11-2 | Penicillium rubens | Class: Eurotiomycetes Order: Eurotiales Family: Trichocomaceae Genus: Penicillium | OR844310 |
Sample | Volume (mL) | Protein (mg) | Specific CAT Activity (U/mg) | Total CAT Activity (U) | Yield (%) | Degree of Purification (Fold) |
---|---|---|---|---|---|---|
Output | 315 | 1.466 | 5.22 | 2410.50 | 100 | 1 |
Ultrafiltration | 24 | 2.465 | 6.02 | 356.14 | 14.77 | 1.15 |
(NH4)2SO4 Saturation | 20 | 1.528 | 18.31 | 559.55 | 23.21 | 3.51 |
Column Q-Sepharose | 7.5 | 0.125 | 513.72 | 481.60 | 19.97 | 98.4 |
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Koleva, Z.; Abrashev, R.; Angelova, M.; Stoyancheva, G.; Spassova, B.; Yovchevska, L.; Dishliyska, V.; Miteva-Staleva, J.; Krumova, E. A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2. Fermentation 2024, 10, 58. https://doi.org/10.3390/fermentation10010058
Koleva Z, Abrashev R, Angelova M, Stoyancheva G, Spassova B, Yovchevska L, Dishliyska V, Miteva-Staleva J, Krumova E. A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2. Fermentation. 2024; 10(1):58. https://doi.org/10.3390/fermentation10010058
Chicago/Turabian StyleKoleva, Zdravka, Radoslav Abrashev, Maria Angelova, Galina Stoyancheva, Boryana Spassova, Lyudmila Yovchevska, Vladislava Dishliyska, Jeny Miteva-Staleva, and Ekaterina Krumova. 2024. "A Novel Extracellular Catalase Produced by the Antarctic Filamentous Fungus Penicillium Rubens III11-2" Fermentation 10, no. 1: 58. https://doi.org/10.3390/fermentation10010058