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Article

Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness under Acetic Acid Stress Involving Pdr18

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iBB—Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal
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Associate Laboratory i4HB—Institute for Health and Bioeconomy at Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal
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Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisbon, Portugal
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BioISI—Biosystems and Integrative Sciences Institute, Faculdade de Ciências, Universidade de Lisboa, Campo Grande 16, 1749-016 Lisbon, Portugal
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Departament of Physics, Faculdade de Ciências, Universidade de Lisboa, Campo Grande 16, 1749-016 Lisbon, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Laurent Dufossé
J. Fungi 2022, 8(2), 103; https://doi.org/10.3390/jof8020103
Received: 29 December 2021 / Revised: 16 January 2022 / Accepted: 18 January 2022 / Published: 21 January 2022
(This article belongs to the Special Issue Yeast Biorefineries)
Acetic acid is a major inhibitory compound in several industrial bioprocesses, in particular in lignocellulosic yeast biorefineries. Cell envelope remodeling, involving cell wall and plasma membrane composition, structure and function, is among the mechanisms behind yeast adaptation and tolerance to stress. Pdr18 is a plasma membrane ABC transporter of the pleiotropic drug resistance family and a reported determinant of acetic acid tolerance mediating ergosterol transport. This study provides evidence for the impact of Pdr18 expression in yeast cell wall during adaptation to acetic acid stress. The time-course of acetic-acid-induced transcriptional activation of cell wall biosynthetic genes (FKS1, BGL2, CHS3, GAS1) and of increased cell wall stiffness and cell wall polysaccharide content in cells with the PDR18 deleted, compared to parental cells, is reported. Despite the robust and more intense adaptive response of the pdr18Δ population, the stress-induced increase of cell wall resistance to lyticase activity was below parental strain levels, and the duration of the period required for intracellular pH recovery from acidification and growth resumption was higher in the less tolerant pdr18Δ population. The ergosterol content, critical for plasma membrane stabilization, suffered a drastic reduction in the first hour of cultivation under acetic acid stress, especially in pdr18Δ cells. Results revealed a crosstalk between plasma membrane ergosterol content and cell wall biophysical properties, suggesting a coordinated response to counteract the deleterious effects of acetic acid. View Full-Text
Keywords: Saccharomyces cerevisiae; adaptation and tolerance to acetic acid; cell envelope; plasma membrane; cell wall stiffness; cell wall remodeling; lipid homeostasis; ergosterol; ABC transporters; Pdr18; intracellular pH Saccharomyces cerevisiae; adaptation and tolerance to acetic acid; cell envelope; plasma membrane; cell wall stiffness; cell wall remodeling; lipid homeostasis; ergosterol; ABC transporters; Pdr18; intracellular pH
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MDPI and ACS Style

Ribeiro, R.A.; Godinho, C.P.; Vitorino, M.V.; Robalo, T.T.; Fernandes, F.; Rodrigues, M.S.; Sá-Correia, I. Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness under Acetic Acid Stress Involving Pdr18. J. Fungi 2022, 8, 103. https://doi.org/10.3390/jof8020103

AMA Style

Ribeiro RA, Godinho CP, Vitorino MV, Robalo TT, Fernandes F, Rodrigues MS, Sá-Correia I. Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness under Acetic Acid Stress Involving Pdr18. Journal of Fungi. 2022; 8(2):103. https://doi.org/10.3390/jof8020103

Chicago/Turabian Style

Ribeiro, Ricardo A., Cláudia P. Godinho, Miguel V. Vitorino, Tiago T. Robalo, Fábio Fernandes, Mário S. Rodrigues, and Isabel Sá-Correia. 2022. "Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness under Acetic Acid Stress Involving Pdr18" Journal of Fungi 8, no. 2: 103. https://doi.org/10.3390/jof8020103

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