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Article

Dexamethasone Creates a Suppressive Microenvironment and Promotes Aspergillus fumigatus Invasion in a Human 3D Epithelial/Immune Respiratory Model

Institute of Hygiene and Medical Microbiology, Medical University of Innsbruck, 6020 Innsbruck, Austria
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Author to whom correspondence should be addressed.
Academic Editor: Joseph M. Bliss
J. Fungi 2021, 7(3), 221; https://doi.org/10.3390/jof7030221
Received: 4 March 2021 / Revised: 12 March 2021 / Accepted: 15 March 2021 / Published: 18 March 2021
(This article belongs to the Special Issue Host Defense against Fungi)
Lung immunity and susceptibility to infections is subject to interactions between the epithelial layer and immune cells residing in the pulmonary space. Aspergillus (A.) fumigatus, the most prevalent pathogenic fungus, affects both upper and lower respiratory tracts of immunocompromised hosts. Several reports implicate corticosteroids as a major risk factor due to their anti-inflammatory and immunosuppressive effects, which are exacerbated by long-term treatment regimens. Here we demonstrate for the first time the influence of dexamethasone when it comes to germination and hyphae formation of A. fumigatus in the presence of macrophages within a highly differentiated air–liquid interphase (ALI) epithelial/immune lung model. We illustrate suppressed mucus production within the highly differentiated 3D respiratory model as well as significantly decreased cilia beat frequencies by dexamethasone treatment. This goes along with corticosteroid-mediated macrophage M2 polarization within the epithelial/immune microenvironment. Therefore, we here showed that corticosteroids promote enhanced fungal growth and invasion A. fumigatus by creating a suppressive environment affecting both epithelial as well as immune cells. View Full-Text
Keywords: dexamethasone; Aspergillus fumigatus; 3D lung model; macrophages dexamethasone; Aspergillus fumigatus; 3D lung model; macrophages
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MDPI and ACS Style

Luvanda, M.K.; Posch, W.; Noureen, A.; Lafon, E.; Zaderer, V.; Lass-Flörl, C.; Wilflingseder, D. Dexamethasone Creates a Suppressive Microenvironment and Promotes Aspergillus fumigatus Invasion in a Human 3D Epithelial/Immune Respiratory Model. J. Fungi 2021, 7, 221. https://doi.org/10.3390/jof7030221

AMA Style

Luvanda MK, Posch W, Noureen A, Lafon E, Zaderer V, Lass-Flörl C, Wilflingseder D. Dexamethasone Creates a Suppressive Microenvironment and Promotes Aspergillus fumigatus Invasion in a Human 3D Epithelial/Immune Respiratory Model. Journal of Fungi. 2021; 7(3):221. https://doi.org/10.3390/jof7030221

Chicago/Turabian Style

Luvanda, Maureen K., Wilfried Posch, Asma Noureen, Eliott Lafon, Viktoria Zaderer, Cornelia Lass-Flörl, and Doris Wilflingseder. 2021. "Dexamethasone Creates a Suppressive Microenvironment and Promotes Aspergillus fumigatus Invasion in a Human 3D Epithelial/Immune Respiratory Model" Journal of Fungi 7, no. 3: 221. https://doi.org/10.3390/jof7030221

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