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Article

Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors

1
Departamento de Genética, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba CEP 13418-900, Brazil
2
Christopher S. Bond Life Sciences Center and Interdisciplinary Plant Group, University of Missouri, Columbia, MO 65211, USA
3
Division of Plant Sciences, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, MO 65211, USA
*
Authors to whom correspondence should be addressed.
J. Fungi 2020, 6(4), 339; https://doi.org/10.3390/jof6040339
Received: 30 October 2020 / Revised: 28 November 2020 / Accepted: 30 November 2020 / Published: 4 December 2020
(This article belongs to the Special Issue Smut Fungi)
Sporisorium scitamineum is a biotrophic fungus causing sugarcane smut disease. In this study, we set up a pipeline and used genomic and dual transcriptomic data previously obtained by our group to identify candidate effectors of S. scitamineum and their expression profiles in infected smut-resistant and susceptible sugarcane plants. The expression profile of different genes after infection in contrasting sugarcane genotypes assessed by RT-qPCR depended on the plant genotypes and disease progression. Three candidate effector genes expressed earlier only in resistant plants, four expressed in both genotypes, and three later in susceptible plants. Ten genes were cloned and transiently expressed in N. benthamiana leaves to determine their subcellular location, while four localized in more than one compartment. Two candidates, g3890 having a nucleoplasmic and mitochondrial location and g5159 targeting the plant cell wall, were selected to obtain their possible corresponding host targets using co-immunoprecipitation (CoIP) experiments and mass spectrometry. Various potential interactors were identified, including subunits of the protein phosphatase 2A and an endochitinase. We investigated the presence of orthologs in sugarcane and using transcriptome data present their expression profiles. Orthologs of sugarcane shared around 70% similarity. Identifying a set of putative fungal effectors and their plant targets provides a valuable resource for functional characterization of the molecular events leading to smut resistance in sugarcane plants and uncovers further opportunities for investigation. View Full-Text
Keywords: effector proteins; subcellular location; fungal effector; sugarcane smut; plant immunity; agroinfiltration; Nicotiana benthamiana effector proteins; subcellular location; fungal effector; sugarcane smut; plant immunity; agroinfiltration; Nicotiana benthamiana
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MDPI and ACS Style

Teixeira-Silva, N.S.; Schaker, P.D.C.; Rody, H.V.S.; Maia, T.; Garner, C.M.; Gassmann, W.; Monteiro-Vitorello, C.B. Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors. J. Fungi 2020, 6, 339. https://doi.org/10.3390/jof6040339

AMA Style

Teixeira-Silva NS, Schaker PDC, Rody HVS, Maia T, Garner CM, Gassmann W, Monteiro-Vitorello CB. Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors. Journal of Fungi. 2020; 6(4):339. https://doi.org/10.3390/jof6040339

Chicago/Turabian Style

Teixeira-Silva, Natália Sousa, Patrícia Dayane Carvalho Schaker, Hugo Vianna Silva Rody, Thiago Maia, Christopher M. Garner, Walter Gassmann, and Claudia Barros Monteiro-Vitorello. 2020. "Leaping into the Unknown World of Sporisorium scitamineum Candidate Effectors" Journal of Fungi 6, no. 4: 339. https://doi.org/10.3390/jof6040339

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