Quantitative Assessment of Grapevine Wood Colonization by the Dieback Fungus Eutypa lata
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Fungal Material and Inoculation
2.3. Re-Isolation Method
2.4. Fungal Biomass Measurement by Quantitative PCR
2.5. Experimental Design
2.6. Data Analysis and Reproducible Research
3. Results
3.1. Wood Colonization in Relation to Aggressiveness and Distance from Inoculation Site (Experiment 1)
3.2. Comparison of Grapevine Cultivars for Their Tolerance to Wood Colonization by E. lata (Experiment 2)
3.3. qPCR as a Tool to Evaluate Wood Colonization by E. lata (Experiment 3)
3.4. Comparison of E. lata Aggressiveness by qPCR (Experiment 4)
4. Discussion
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Stroma | Location | Year of Isolation | High-Aggressive Isolate | Low-Aggressive Isolate |
---|---|---|---|---|
AM78 | Aigues-Mortes | 1992 | AM78-4 | AM78-1 |
BX1 | Bordeaux | 1990 | BX1-10 | BX1-5 |
CM96 | Chambolle-Musigny | 1993 | CM96-7 | CM96-6 |
VL11 | Villeneuve-lès-Maguelone | 1992 | VL11-12 | VL11-3 |
Primers | Sequences (5’ -> 3’) | Length (nt) | Target | PCR Product Size | Organism | Source | |
---|---|---|---|---|---|---|---|
Act3_37_F | TCCTTGCCTTGCGTCATCTAT | Fwd | 21 | Actin | 72 bp | V. vinifera | Gatto et al. [36] |
Act3_38_R | CACCAATCACTCTCCTGCTACAA | Rev | 23 | ||||
EL_b-tub_33_F | CTGGCAATGCTAACTCGCCGCT | Fwd | 22 | β-tubulin | 90 bp | E. lata | This study |
EL_b-tub_34_R | CGAGGAACATACTTGTTGCCGGAC | Rev | 24 |
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Moisy, C.; Berger, G.; Flutre, T.; Le Cunff, L.; Péros, J.-P. Quantitative Assessment of Grapevine Wood Colonization by the Dieback Fungus Eutypa lata. J. Fungi 2017, 3, 21. https://doi.org/10.3390/jof3020021
Moisy C, Berger G, Flutre T, Le Cunff L, Péros J-P. Quantitative Assessment of Grapevine Wood Colonization by the Dieback Fungus Eutypa lata. Journal of Fungi. 2017; 3(2):21. https://doi.org/10.3390/jof3020021
Chicago/Turabian StyleMoisy, Cédric, Gilles Berger, Timothée Flutre, Loïc Le Cunff, and Jean-Pierre Péros. 2017. "Quantitative Assessment of Grapevine Wood Colonization by the Dieback Fungus Eutypa lata" Journal of Fungi 3, no. 2: 21. https://doi.org/10.3390/jof3020021
APA StyleMoisy, C., Berger, G., Flutre, T., Le Cunff, L., & Péros, J. -P. (2017). Quantitative Assessment of Grapevine Wood Colonization by the Dieback Fungus Eutypa lata. Journal of Fungi, 3(2), 21. https://doi.org/10.3390/jof3020021