Distribution of Coronavirus Receptors in the Swine Respiratory and Intestinal Tract
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. The Study
2.2. Sample Collection
2.3. Quantitative PCR
2.4. Immunohistochemistry
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trachea | Bronchiole | Lung | Duodenum | Jejunum | Ileum | Cecum | Colon | Rectum | ||
---|---|---|---|---|---|---|---|---|---|---|
Immunohistochemistry | Viruses Showing Affinity to These Receptors | |||||||||
APN/CD13 | + (2/2) | + (2/2) | + (2/2) | + (3/3) | + (3/3) | + (3/3) | + (3/3) | + (3/3) | + (3/3) | FCoV, CCoV, TGEV, PRCV, PEDV, PDCoV |
DPP4/CD26 | + (2/2) | + (2/2) | + (2/2) | + (3/3) | + (3/3) | + (3/3) | + (2/3) | + (2/3) | + (2/3) | MERS-CoV |
ACE2 | + ** (2/2) | + (2/2) | + (2/2) | + * (3/3) | + * (3/3) | + * (3/3) | + * (2/3) | − (3/3) | + (2/3) | HCoV-NL63, SARS-CoV, SARS-CoV2 |
CEACAM1/CD66a | + (2/2) | + (2/2) | + (2/2) | + # (3/3) | + # (3/3) | + # (3/3) | + # (2/3) | + # (2/3) | + # (2/3) | MHV |
Gene expression (NCBI reference) | Primer sequences | |||||||||
APN/CD13 (NM_214277.1) | + (2/2) | Not performed | + (2/2) | + (3/3) | + (3/3) | + (3/3) | + (3/3) | + (3/3) | + (3/3) | For Primer 5′-CACGACACAGATGCAGTCTACAGA-3′ Rev Primer 5′-TGTTGAACGTGGCCTTCATG-3′ |
DPP4/CD26 (NM_214257.1) | + (2/2) | Not performed | + (2/2) | + (3/3) | + (3/3) | + (3/3) | + (1/3) | + (2/3) | + (2/3) | For Primer 5′-ACCAGGACTCTCAGCCCAAA-3′ Rev Primer 5′-ACAAGTAGTGATCCCCTATTAACACAGA-3′ |
ACE2 (NM_001123070.1) | + (2/2) | Not performed | + (2/2) | + (2/3) | + (2/3) | + (3/3) | − (3/3) | + (1/3) | − (3/3) | For Primer 5′-GGGTGGTGATGGGATTGGTA-3′ Rev Primer 5′-TTGCTTTTTCTTCCTTCGATCTCT-3′ |
CEACAM1/CD66a (XM_021094420.1) | + (2/2) | Not performed | + (2/2) | + (2/3) | − (3/3) | + (2/3) | + (1/3) | + (3/3) | + (3/3) | For Primer 5′-TGCTCGCAGAGAGGATAAAACTG-3′ Rev Primer 5′-GGCCTCGCACTGATAATTCC-3′ |
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Nelli, R.K.; Roth, J.A.; Gimenez-Lirola, L.G. Distribution of Coronavirus Receptors in the Swine Respiratory and Intestinal Tract. Vet. Sci. 2022, 9, 500. https://doi.org/10.3390/vetsci9090500
Nelli RK, Roth JA, Gimenez-Lirola LG. Distribution of Coronavirus Receptors in the Swine Respiratory and Intestinal Tract. Veterinary Sciences. 2022; 9(9):500. https://doi.org/10.3390/vetsci9090500
Chicago/Turabian StyleNelli, Rahul Kumar, James Allen Roth, and Luis Gabriel Gimenez-Lirola. 2022. "Distribution of Coronavirus Receptors in the Swine Respiratory and Intestinal Tract" Veterinary Sciences 9, no. 9: 500. https://doi.org/10.3390/vetsci9090500