Canine Stem Cell Conditioned Media Accelerates Epithelial Migration in the Canine Tympanic Membrane
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collection of Canine Adipose Tissue-Derived Mesenchymal Stem Cells (cAD-MSCs)
2.2. Collection of Canine Keratinocyte-Conditioned Media (cKCM)
2.3. Quantification of Basic Fibroblast Growth Factor (bFGF), Interleukin-6 (IL-6), and Vascular Endothelial Growth Factor-A (VEGF-A) in SCCM
2.4. Laboratory Animals
2.5. Evaluation of the Ear Canals
2.6. Ink Spotting, Repeated Re-Evaluation, and cAD-MSCCM Application
2.7. Malleus Width Measurement for Calibration
2.8. Evaluation of the TM EM Rate
2.9. Statistical Analysis
3. Results
3.1. Levels of bFGF, IL-6, and VEGF-A in cAD-MSCCM and cKCM
3.2. Malleus Width Measurement and Calibration
3.3. Evaluation of the TM EM Rate and Effect of cAD-MSCCM
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Width of the Malleus (μm) | ||
---|---|---|
Left Ear (LE) | Right Ear (RE) | |
Beagle 1 | 1129.4 | 957.3 |
Beagle 2 | 1146.5 | 958.0 |
Beagle 3 | 1217.9 | 1011.3 |
Beagle 4 | 763.5 | 993.5 |
Total | 1064.0 ± 204.2 | 980.0 ± 26.8 |
1022 ± 142.2 |
TM EM Rate per Day (μm/d) | ||
---|---|---|
Side of Ear | Treatment Group | Control Group |
LE | 208.7 ± 62.1 | 128.8 ± 26.0 |
RE | 216.3 ± 79.9 | 136.3 ± 37.8 |
Total | 212.5 ± 66.4 | 133.1 ± 30.9 |
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Suh, H.; Kim, S.; Oh, T.; Bae, S. Canine Stem Cell Conditioned Media Accelerates Epithelial Migration in the Canine Tympanic Membrane. Vet. Sci. 2022, 9, 69. https://doi.org/10.3390/vetsci9020069
Suh H, Kim S, Oh T, Bae S. Canine Stem Cell Conditioned Media Accelerates Epithelial Migration in the Canine Tympanic Membrane. Veterinary Sciences. 2022; 9(2):69. https://doi.org/10.3390/vetsci9020069
Chicago/Turabian StyleSuh, Hyerin, Suhyun Kim, Taeho Oh, and Seulgi Bae. 2022. "Canine Stem Cell Conditioned Media Accelerates Epithelial Migration in the Canine Tympanic Membrane" Veterinary Sciences 9, no. 2: 69. https://doi.org/10.3390/vetsci9020069